PICKLE acts throughout the plant to repress expression of embryonic traits and may play a role in Giberellin-Dependent responses

J. Henderson, H.C. Li, A.P. Mordhorst, J. Romero-Severson, J.C. Cheng, J. Robey, Z.R. Sung, S.C. de Vries, J. Ogas

Research output: Contribution to journalArticleAcademicpeer-review

109 Citations (Scopus)

Abstract

A seed marks the transition between two developmental states; a plant is an embryo during seed formation, whereas it is a seedling after emergence from the seed. Two factors have been identified in Arabidopsis that play a role in establishment of repression of the embryonic state: PKL (PICKLE), which codes for a putative CHD3 chromatin remodeling factor, and gibberellin (GA), a plant growth regulator. Previous observations have also suggested that PKL mediates some aspects of GA responsiveness in the adult plant. To investigate possible mechanisms by which PKL and GA might act to repress the embryonic state, we further characterized the ability of PKL and GA to repress embryonic traits and reexamined the role of PKL in mediating GA-dependent responses. We found that PKL acts throughout the seedling to repress expression of embryonic traits. Although the ability of pkl seedlings to express embryonic traits is strongly induced by inhibiting GA biosynthesis, it is only marginally responsive to abscisic acid and SPY (SPINDLY), factors that have previously been demonstrated to inhibit GA-dependent responses during germination. We also observed that pkl plants exhibit the phenotypic hallmarks of a mutation in a positive regulator of a GA response pathway including reduced GA responsiveness and increased synthesis of bioactive GAs. These observations indicate that PKL may mediate a subset of GA-dependent responses during shoot development.
Original languageEnglish
Pages (from-to)995-1005
JournalPlant Physiology
Volume134
Issue number3
DOIs
Publication statusPublished - 2004

Fingerprint

Seeds
Seedlings
seedlings
seeds
mature plants
Gibberellins
plant growth substances
gibberellins
embryo (plant)
Abscisic Acid
Plant Growth Regulators
Chromatin Assembly and Disassembly
chromatin
abscisic acid
Germination
Arabidopsis
biosynthesis
germination
mutation
synthesis

Keywords

  • arabidopsis seed-germination
  • thaliana l heynh
  • histone deacetylase
  • somatic embryogenesis
  • signal-transduction
  • leafy cotyledon1
  • gene-expression
  • complex
  • mutants
  • locus

Cite this

Henderson, J., Li, H. C., Mordhorst, A. P., Romero-Severson, J., Cheng, J. C., Robey, J., ... Ogas, J. (2004). PICKLE acts throughout the plant to repress expression of embryonic traits and may play a role in Giberellin-Dependent responses. Plant Physiology, 134(3), 995-1005. https://doi.org/10.1104/pp.103.030148
Henderson, J. ; Li, H.C. ; Mordhorst, A.P. ; Romero-Severson, J. ; Cheng, J.C. ; Robey, J. ; Sung, Z.R. ; de Vries, S.C. ; Ogas, J. / PICKLE acts throughout the plant to repress expression of embryonic traits and may play a role in Giberellin-Dependent responses. In: Plant Physiology. 2004 ; Vol. 134, No. 3. pp. 995-1005.
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title = "PICKLE acts throughout the plant to repress expression of embryonic traits and may play a role in Giberellin-Dependent responses",
abstract = "A seed marks the transition between two developmental states; a plant is an embryo during seed formation, whereas it is a seedling after emergence from the seed. Two factors have been identified in Arabidopsis that play a role in establishment of repression of the embryonic state: PKL (PICKLE), which codes for a putative CHD3 chromatin remodeling factor, and gibberellin (GA), a plant growth regulator. Previous observations have also suggested that PKL mediates some aspects of GA responsiveness in the adult plant. To investigate possible mechanisms by which PKL and GA might act to repress the embryonic state, we further characterized the ability of PKL and GA to repress embryonic traits and reexamined the role of PKL in mediating GA-dependent responses. We found that PKL acts throughout the seedling to repress expression of embryonic traits. Although the ability of pkl seedlings to express embryonic traits is strongly induced by inhibiting GA biosynthesis, it is only marginally responsive to abscisic acid and SPY (SPINDLY), factors that have previously been demonstrated to inhibit GA-dependent responses during germination. We also observed that pkl plants exhibit the phenotypic hallmarks of a mutation in a positive regulator of a GA response pathway including reduced GA responsiveness and increased synthesis of bioactive GAs. These observations indicate that PKL may mediate a subset of GA-dependent responses during shoot development.",
keywords = "arabidopsis seed-germination, thaliana l heynh, histone deacetylase, somatic embryogenesis, signal-transduction, leafy cotyledon1, gene-expression, complex, mutants, locus",
author = "J. Henderson and H.C. Li and A.P. Mordhorst and J. Romero-Severson and J.C. Cheng and J. Robey and Z.R. Sung and {de Vries}, S.C. and J. Ogas",
year = "2004",
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language = "English",
volume = "134",
pages = "995--1005",
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Henderson, J, Li, HC, Mordhorst, AP, Romero-Severson, J, Cheng, JC, Robey, J, Sung, ZR, de Vries, SC & Ogas, J 2004, 'PICKLE acts throughout the plant to repress expression of embryonic traits and may play a role in Giberellin-Dependent responses', Plant Physiology, vol. 134, no. 3, pp. 995-1005. https://doi.org/10.1104/pp.103.030148

PICKLE acts throughout the plant to repress expression of embryonic traits and may play a role in Giberellin-Dependent responses. / Henderson, J.; Li, H.C.; Mordhorst, A.P.; Romero-Severson, J.; Cheng, J.C.; Robey, J.; Sung, Z.R.; de Vries, S.C.; Ogas, J.

In: Plant Physiology, Vol. 134, No. 3, 2004, p. 995-1005.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - PICKLE acts throughout the plant to repress expression of embryonic traits and may play a role in Giberellin-Dependent responses

AU - Henderson, J.

AU - Li, H.C.

AU - Mordhorst, A.P.

AU - Romero-Severson, J.

AU - Cheng, J.C.

AU - Robey, J.

AU - Sung, Z.R.

AU - de Vries, S.C.

AU - Ogas, J.

PY - 2004

Y1 - 2004

N2 - A seed marks the transition between two developmental states; a plant is an embryo during seed formation, whereas it is a seedling after emergence from the seed. Two factors have been identified in Arabidopsis that play a role in establishment of repression of the embryonic state: PKL (PICKLE), which codes for a putative CHD3 chromatin remodeling factor, and gibberellin (GA), a plant growth regulator. Previous observations have also suggested that PKL mediates some aspects of GA responsiveness in the adult plant. To investigate possible mechanisms by which PKL and GA might act to repress the embryonic state, we further characterized the ability of PKL and GA to repress embryonic traits and reexamined the role of PKL in mediating GA-dependent responses. We found that PKL acts throughout the seedling to repress expression of embryonic traits. Although the ability of pkl seedlings to express embryonic traits is strongly induced by inhibiting GA biosynthesis, it is only marginally responsive to abscisic acid and SPY (SPINDLY), factors that have previously been demonstrated to inhibit GA-dependent responses during germination. We also observed that pkl plants exhibit the phenotypic hallmarks of a mutation in a positive regulator of a GA response pathway including reduced GA responsiveness and increased synthesis of bioactive GAs. These observations indicate that PKL may mediate a subset of GA-dependent responses during shoot development.

AB - A seed marks the transition between two developmental states; a plant is an embryo during seed formation, whereas it is a seedling after emergence from the seed. Two factors have been identified in Arabidopsis that play a role in establishment of repression of the embryonic state: PKL (PICKLE), which codes for a putative CHD3 chromatin remodeling factor, and gibberellin (GA), a plant growth regulator. Previous observations have also suggested that PKL mediates some aspects of GA responsiveness in the adult plant. To investigate possible mechanisms by which PKL and GA might act to repress the embryonic state, we further characterized the ability of PKL and GA to repress embryonic traits and reexamined the role of PKL in mediating GA-dependent responses. We found that PKL acts throughout the seedling to repress expression of embryonic traits. Although the ability of pkl seedlings to express embryonic traits is strongly induced by inhibiting GA biosynthesis, it is only marginally responsive to abscisic acid and SPY (SPINDLY), factors that have previously been demonstrated to inhibit GA-dependent responses during germination. We also observed that pkl plants exhibit the phenotypic hallmarks of a mutation in a positive regulator of a GA response pathway including reduced GA responsiveness and increased synthesis of bioactive GAs. These observations indicate that PKL may mediate a subset of GA-dependent responses during shoot development.

KW - arabidopsis seed-germination

KW - thaliana l heynh

KW - histone deacetylase

KW - somatic embryogenesis

KW - signal-transduction

KW - leafy cotyledon1

KW - gene-expression

KW - complex

KW - mutants

KW - locus

U2 - 10.1104/pp.103.030148

DO - 10.1104/pp.103.030148

M3 - Article

VL - 134

SP - 995

EP - 1005

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -