Phytophthora infestans has a plethora of phospholipase D enzymes including a subclass that has extracellular activity

H.J.G. Meijer, H.H. Hassen, F. Govers

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

In eukaryotes phospholipase D (PLD) is involved in many cellular processes. Currently little is known about PLDs in oomycetes. Here we report that the oomycete plant pathogen Phytophthora infestans has a large repertoire of PLDs divided over six subfamilies: PXPH-PLD, PXTM-PLD, TM-PLD, PLD-likes, and type A and B sPLD-likes. Since the latter have signal peptides we developed a method using metabolically labelled phospholipids to monitor if P. infestans secretes PLD. In extracellular medium of ten P. infestans strains PLD activity was detected as demonstrated by the production of phosphatidic acid and the PLD specific marker phosphatidylalcohol
Original languageEnglish
Article numbere17767
Number of pages12
JournalPLoS ONE
Volume6
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • phosphatidic-acid
  • cytoskeleton
  • activation

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