Physicochemical properties of pectins from okra (Abelmoschus esculentus (L.) Moench)

N. Sengkhamparn, L.M.C. Sagis, R.J. de Vries, H.A. Schols, T. Sajjaanantakul, A.G.J. Voragen

Research output: Contribution to journalArticleAcademicpeer-review

89 Citations (Scopus)

Abstract

Okra pectin obtained by hot buffer extraction (HBSS) consists of an unusual pectic rhamnogalacturonan I structure in which acetyl groups and alpha galactose residues are substituted on rhamnose residues within the backbone. The okra Chelating agent Soluble Solids (CHSS) pectin consists of slightly different structures since relatively more homogalacturonan is present within the macromolecule and the rhamnogalacturonan I segments carry slightly longer side chains. The rheological properties of both okra pectins were examined under various conditions in order to understand the unusual slimy behaviour of okra pectins. The viscosity of the okra HBSS pectin was 5–8 times higher than the viscosity of the okra CHSS pectin. The okra HBSS pectin showed an elastic behaviour (G' > G¿) over a wide range of frequencies (10-1–10 Hz), at a strain of 10%, while okra CHSS and saponified okra HBSS/CHSS pectin showed predominantly viscous responses (G' <G¿) over the same frequency range. The results suggest that the structural variation within the okra pectins greatly affect their rheological behaviour and it is suggested that acetylation of the pectin plays an important role through hydrophobic associations. Dynamic light scattering was used to study the association behaviour of both okra pectins at low concentration (0.001–0.1% w/w). Results showed that the saponified okra pectins did not exhibit a tendency to aggregate in the concentration range studied, whereas both non saponified samples showed a substantial degree of association. These results suggest that the unusual slimy behaviour of the non saponified samples may be related to the tendency of these pectins to associate, driven by hydrophobic interactions.
LanguageEnglish
Pages35-41
JournalFood Hydrocolloids
Volume24
DOIs
Publication statusPublished - 2010

Fingerprint

Abelmoschus
Pectins
Abelmoschus esculentus
okra
pectins
physicochemical properties
Chelation
Chelating Agents
chelating agents
total soluble solids
Association reactions
Viscosity
Acetylation
Rhamnose
Dynamic light scattering
Macromolecules
pectin
Galactose
viscosity
Buffers

Keywords

  • rheological properties
  • flow
  • polysaccharides

Cite this

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title = "Physicochemical properties of pectins from okra (Abelmoschus esculentus (L.) Moench)",
abstract = "Okra pectin obtained by hot buffer extraction (HBSS) consists of an unusual pectic rhamnogalacturonan I structure in which acetyl groups and alpha galactose residues are substituted on rhamnose residues within the backbone. The okra Chelating agent Soluble Solids (CHSS) pectin consists of slightly different structures since relatively more homogalacturonan is present within the macromolecule and the rhamnogalacturonan I segments carry slightly longer side chains. The rheological properties of both okra pectins were examined under various conditions in order to understand the unusual slimy behaviour of okra pectins. The viscosity of the okra HBSS pectin was 5–8 times higher than the viscosity of the okra CHSS pectin. The okra HBSS pectin showed an elastic behaviour (G' > G¿) over a wide range of frequencies (10-1–10 Hz), at a strain of 10{\%}, while okra CHSS and saponified okra HBSS/CHSS pectin showed predominantly viscous responses (G' <G¿) over the same frequency range. The results suggest that the structural variation within the okra pectins greatly affect their rheological behaviour and it is suggested that acetylation of the pectin plays an important role through hydrophobic associations. Dynamic light scattering was used to study the association behaviour of both okra pectins at low concentration (0.001–0.1{\%} w/w). Results showed that the saponified okra pectins did not exhibit a tendency to aggregate in the concentration range studied, whereas both non saponified samples showed a substantial degree of association. These results suggest that the unusual slimy behaviour of the non saponified samples may be related to the tendency of these pectins to associate, driven by hydrophobic interactions.",
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author = "N. Sengkhamparn and L.M.C. Sagis and {de Vries}, R.J. and H.A. Schols and T. Sajjaanantakul and A.G.J. Voragen",
year = "2010",
doi = "10.1016/j.foodhyd.2009.07.007",
language = "English",
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Physicochemical properties of pectins from okra (Abelmoschus esculentus (L.) Moench). / Sengkhamparn, N.; Sagis, L.M.C.; de Vries, R.J.; Schols, H.A.; Sajjaanantakul, T.; Voragen, A.G.J.

In: Food Hydrocolloids, Vol. 24, 2010, p. 35-41.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Physicochemical properties of pectins from okra (Abelmoschus esculentus (L.) Moench)

AU - Sengkhamparn, N.

AU - Sagis, L.M.C.

AU - de Vries, R.J.

AU - Schols, H.A.

AU - Sajjaanantakul, T.

AU - Voragen, A.G.J.

PY - 2010

Y1 - 2010

N2 - Okra pectin obtained by hot buffer extraction (HBSS) consists of an unusual pectic rhamnogalacturonan I structure in which acetyl groups and alpha galactose residues are substituted on rhamnose residues within the backbone. The okra Chelating agent Soluble Solids (CHSS) pectin consists of slightly different structures since relatively more homogalacturonan is present within the macromolecule and the rhamnogalacturonan I segments carry slightly longer side chains. The rheological properties of both okra pectins were examined under various conditions in order to understand the unusual slimy behaviour of okra pectins. The viscosity of the okra HBSS pectin was 5–8 times higher than the viscosity of the okra CHSS pectin. The okra HBSS pectin showed an elastic behaviour (G' > G¿) over a wide range of frequencies (10-1–10 Hz), at a strain of 10%, while okra CHSS and saponified okra HBSS/CHSS pectin showed predominantly viscous responses (G' <G¿) over the same frequency range. The results suggest that the structural variation within the okra pectins greatly affect their rheological behaviour and it is suggested that acetylation of the pectin plays an important role through hydrophobic associations. Dynamic light scattering was used to study the association behaviour of both okra pectins at low concentration (0.001–0.1% w/w). Results showed that the saponified okra pectins did not exhibit a tendency to aggregate in the concentration range studied, whereas both non saponified samples showed a substantial degree of association. These results suggest that the unusual slimy behaviour of the non saponified samples may be related to the tendency of these pectins to associate, driven by hydrophobic interactions.

AB - Okra pectin obtained by hot buffer extraction (HBSS) consists of an unusual pectic rhamnogalacturonan I structure in which acetyl groups and alpha galactose residues are substituted on rhamnose residues within the backbone. The okra Chelating agent Soluble Solids (CHSS) pectin consists of slightly different structures since relatively more homogalacturonan is present within the macromolecule and the rhamnogalacturonan I segments carry slightly longer side chains. The rheological properties of both okra pectins were examined under various conditions in order to understand the unusual slimy behaviour of okra pectins. The viscosity of the okra HBSS pectin was 5–8 times higher than the viscosity of the okra CHSS pectin. The okra HBSS pectin showed an elastic behaviour (G' > G¿) over a wide range of frequencies (10-1–10 Hz), at a strain of 10%, while okra CHSS and saponified okra HBSS/CHSS pectin showed predominantly viscous responses (G' <G¿) over the same frequency range. The results suggest that the structural variation within the okra pectins greatly affect their rheological behaviour and it is suggested that acetylation of the pectin plays an important role through hydrophobic associations. Dynamic light scattering was used to study the association behaviour of both okra pectins at low concentration (0.001–0.1% w/w). Results showed that the saponified okra pectins did not exhibit a tendency to aggregate in the concentration range studied, whereas both non saponified samples showed a substantial degree of association. These results suggest that the unusual slimy behaviour of the non saponified samples may be related to the tendency of these pectins to associate, driven by hydrophobic interactions.

KW - rheological properties

KW - flow

KW - polysaccharides

U2 - 10.1016/j.foodhyd.2009.07.007

DO - 10.1016/j.foodhyd.2009.07.007

M3 - Article

VL - 24

SP - 35

EP - 41

JO - Food Hydrocolloids

T2 - Food Hydrocolloids

JF - Food Hydrocolloids

SN - 0268-005X

ER -