Physically triggered morphology changes in a novel Acremonium isolate cultivated in precisely engineered microfabricated environments

Laura Catón, Andrey Yurkov, Marcel Giesbers, Jan Dijksterhuis, Colin J. Ingham

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Fungi are strongly affected by their physical environment. Microfabrication offers the possibility of creating new culture environments and ecosystems with defined characteristics. Here, we report the isolation of a novel member of the fungal genus Acremonium using a microengineered cultivation chip. This isolate was unusual in that it organizes into macroscopic structures when initially cultivated within microwells with a porous aluminum oxide (PAO) base. These "templated mycelial bundles" (TMB) were formed from masses of parallel hyphae with side branching suppressed. TMB were highly hydrated, facilitating the passive movement of solutes along the bundle. By using a range of culture chips, it was deduced that the critical factors in triggering the TMB were growth in microwells from 50 to 300 μm in diameter with a PAO base. Cultivation experiments, using spores and pigments as tracking agents, indicate that bulk growth of the TMB occurs at the base. TMB morphology is highly coherent and is maintained after growing out of the microwells. TMB can explore their environment by developing unbundled lateral hyphae; TMB only followed if nutrients were available. Because of the ease of fabricating numerous microstructures, we suggest this is a productive approach for exploring morphology and growth in multicellular microorganisms and microbial communities.
LanguageEnglish
Article number1269
JournalFrontiers in Microbiology
Volume8
Issue numberJUL
DOIs
Publication statusPublished - 2017

Fingerprint

Acremonium
Hyphae
Aluminum Oxide
Growth
Microtechnology
Spores
Ecosystem
Fungi
Food

Keywords

  • Cultivation chips
  • Fungi
  • Growth on surfaces
  • Mycelial organization
  • Simulated environments

Cite this

@article{d6fb60fe433c44e197ed4729779f6a37,
title = "Physically triggered morphology changes in a novel Acremonium isolate cultivated in precisely engineered microfabricated environments",
abstract = "Fungi are strongly affected by their physical environment. Microfabrication offers the possibility of creating new culture environments and ecosystems with defined characteristics. Here, we report the isolation of a novel member of the fungal genus Acremonium using a microengineered cultivation chip. This isolate was unusual in that it organizes into macroscopic structures when initially cultivated within microwells with a porous aluminum oxide (PAO) base. These {"}templated mycelial bundles{"} (TMB) were formed from masses of parallel hyphae with side branching suppressed. TMB were highly hydrated, facilitating the passive movement of solutes along the bundle. By using a range of culture chips, it was deduced that the critical factors in triggering the TMB were growth in microwells from 50 to 300 μm in diameter with a PAO base. Cultivation experiments, using spores and pigments as tracking agents, indicate that bulk growth of the TMB occurs at the base. TMB morphology is highly coherent and is maintained after growing out of the microwells. TMB can explore their environment by developing unbundled lateral hyphae; TMB only followed if nutrients were available. Because of the ease of fabricating numerous microstructures, we suggest this is a productive approach for exploring morphology and growth in multicellular microorganisms and microbial communities.",
keywords = "Cultivation chips, Fungi, Growth on surfaces, Mycelial organization, Simulated environments",
author = "Laura Cat{\'o}n and Andrey Yurkov and Marcel Giesbers and Jan Dijksterhuis and Ingham, {Colin J.}",
year = "2017",
doi = "10.3389/fmicb.2017.01269",
language = "English",
volume = "8",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers",
number = "JUL",

}

Physically triggered morphology changes in a novel Acremonium isolate cultivated in precisely engineered microfabricated environments. / Catón, Laura; Yurkov, Andrey; Giesbers, Marcel; Dijksterhuis, Jan; Ingham, Colin J.

In: Frontiers in Microbiology, Vol. 8, No. JUL, 1269, 2017.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Physically triggered morphology changes in a novel Acremonium isolate cultivated in precisely engineered microfabricated environments

AU - Catón, Laura

AU - Yurkov, Andrey

AU - Giesbers, Marcel

AU - Dijksterhuis, Jan

AU - Ingham, Colin J.

PY - 2017

Y1 - 2017

N2 - Fungi are strongly affected by their physical environment. Microfabrication offers the possibility of creating new culture environments and ecosystems with defined characteristics. Here, we report the isolation of a novel member of the fungal genus Acremonium using a microengineered cultivation chip. This isolate was unusual in that it organizes into macroscopic structures when initially cultivated within microwells with a porous aluminum oxide (PAO) base. These "templated mycelial bundles" (TMB) were formed from masses of parallel hyphae with side branching suppressed. TMB were highly hydrated, facilitating the passive movement of solutes along the bundle. By using a range of culture chips, it was deduced that the critical factors in triggering the TMB were growth in microwells from 50 to 300 μm in diameter with a PAO base. Cultivation experiments, using spores and pigments as tracking agents, indicate that bulk growth of the TMB occurs at the base. TMB morphology is highly coherent and is maintained after growing out of the microwells. TMB can explore their environment by developing unbundled lateral hyphae; TMB only followed if nutrients were available. Because of the ease of fabricating numerous microstructures, we suggest this is a productive approach for exploring morphology and growth in multicellular microorganisms and microbial communities.

AB - Fungi are strongly affected by their physical environment. Microfabrication offers the possibility of creating new culture environments and ecosystems with defined characteristics. Here, we report the isolation of a novel member of the fungal genus Acremonium using a microengineered cultivation chip. This isolate was unusual in that it organizes into macroscopic structures when initially cultivated within microwells with a porous aluminum oxide (PAO) base. These "templated mycelial bundles" (TMB) were formed from masses of parallel hyphae with side branching suppressed. TMB were highly hydrated, facilitating the passive movement of solutes along the bundle. By using a range of culture chips, it was deduced that the critical factors in triggering the TMB were growth in microwells from 50 to 300 μm in diameter with a PAO base. Cultivation experiments, using spores and pigments as tracking agents, indicate that bulk growth of the TMB occurs at the base. TMB morphology is highly coherent and is maintained after growing out of the microwells. TMB can explore their environment by developing unbundled lateral hyphae; TMB only followed if nutrients were available. Because of the ease of fabricating numerous microstructures, we suggest this is a productive approach for exploring morphology and growth in multicellular microorganisms and microbial communities.

KW - Cultivation chips

KW - Fungi

KW - Growth on surfaces

KW - Mycelial organization

KW - Simulated environments

U2 - 10.3389/fmicb.2017.01269

DO - 10.3389/fmicb.2017.01269

M3 - Article

VL - 8

JO - Frontiers in Microbiology

T2 - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - JUL

M1 - 1269

ER -