Phylogeographic distribution of human and hare Francisella tularenses susp. holarctica strains in the Netherlands and its pathology in European brown hares (Lepus europaeus)

M.G.J. Koene, Jolianne M. Rijks, Miriam Maas, R.C. Ruuls, M.Y. Engelsma, P.W. van Tulden, Marja Kik, Jooske IJzer, Daan Notermans, Maaike de Vries, Ewout Fanoy, Roan Pijnacker, Marcel A.H. Spierenburg, Herjan Bavelaar, Hanneke Berkhout, Sanjay Sankatsing, Rob Diepersloot, Kerstin Myrtennas, Malin Granberg, Mats Forsman & 2 others H.I.J. Roest, Andrea Gröne

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Sequence-based typing of Francisella tularensis has led to insights in the evolutionary developments of tularemia. In Europe, two major basal clades of F. tularensis subsp. holarctica exist, with a distinct geographical distribution. Basal clade B.6 is primarily found in Western Europe, while basal clade B.12 occurs predominantly in the central and eastern parts of Europe. There are indications that tularemia is geographically expanding and that strains from the two clades might differ in pathogenicity, with basal clade B.6 strains being potentially more virulent than basal clade B.12. This study provides information on genotypes detected in the Netherlands during 2011–2017. Data are presented for seven autochthonous human cases and for 29 European brown hares (Lepus europaeus) with laboratory confirmed tularemia. Associated disease patterns are described for 25 European brown hares which underwent post-mortem examination. The basal clades B.6 and B.12 are present both in humans and in European brown hares in the Netherlands, with a patchy geographical distribution. For both genotypes the main pathological findings in hares associated with tularemia were severe (sub)acute necrotizing hepatitis and splenitis as well as necrotizing lesions and hemorrhages in several other organs. Pneumonia was significantly more common in the B.6 than in the B.12 cases. In conclusion, the two major basal clades present in different parts in Europe are both present in the Netherlands. In hares found dead, both genotypes were associated with severe acute disease affecting multiple organs. Hepatitis and splenitis were common pathological findings in hares infected with either genotype, but pneumonia occurred significantly more frequently in hares infected with the B.6 genotype compared to hares infected with the B.12 genotype.
LanguageEnglish
Article number11
Number of pages11
JournalFrontiers in Cellular and Infection Microbiology
Volume9
DOIs
Publication statusPublished - 11 Feb 2019

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Francisella
Hares
Netherlands
Pathology
Tularemia
Genotype
Francisella tularensis
Hepatitis
Pneumonia
Eastern Europe
Acute Disease
Virulence
Autopsy

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Koene, M.G.J. ; Rijks, Jolianne M. ; Maas, Miriam ; Ruuls, R.C. ; Engelsma, M.Y. ; van Tulden, P.W. ; Kik, Marja ; IJzer, Jooske ; Notermans, Daan ; de Vries, Maaike ; Fanoy, Ewout ; Pijnacker, Roan ; Spierenburg, Marcel A.H. ; Bavelaar, Herjan ; Berkhout, Hanneke ; Sankatsing, Sanjay ; Diepersloot, Rob ; Myrtennas, Kerstin ; Granberg, Malin ; Forsman, Mats ; Roest, H.I.J. ; Gröne, Andrea. / Phylogeographic distribution of human and hare Francisella tularenses susp. holarctica strains in the Netherlands and its pathology in European brown hares (Lepus europaeus). In: Frontiers in Cellular and Infection Microbiology. 2019 ; Vol. 9.
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title = "Phylogeographic distribution of human and hare Francisella tularenses susp. holarctica strains in the Netherlands and its pathology in European brown hares (Lepus europaeus)",
abstract = "Sequence-based typing of Francisella tularensis has led to insights in the evolutionary developments of tularemia. In Europe, two major basal clades of F. tularensis subsp. holarctica exist, with a distinct geographical distribution. Basal clade B.6 is primarily found in Western Europe, while basal clade B.12 occurs predominantly in the central and eastern parts of Europe. There are indications that tularemia is geographically expanding and that strains from the two clades might differ in pathogenicity, with basal clade B.6 strains being potentially more virulent than basal clade B.12. This study provides information on genotypes detected in the Netherlands during 2011–2017. Data are presented for seven autochthonous human cases and for 29 European brown hares (Lepus europaeus) with laboratory confirmed tularemia. Associated disease patterns are described for 25 European brown hares which underwent post-mortem examination. The basal clades B.6 and B.12 are present both in humans and in European brown hares in the Netherlands, with a patchy geographical distribution. For both genotypes the main pathological findings in hares associated with tularemia were severe (sub)acute necrotizing hepatitis and splenitis as well as necrotizing lesions and hemorrhages in several other organs. Pneumonia was significantly more common in the B.6 than in the B.12 cases. In conclusion, the two major basal clades present in different parts in Europe are both present in the Netherlands. In hares found dead, both genotypes were associated with severe acute disease affecting multiple organs. Hepatitis and splenitis were common pathological findings in hares infected with either genotype, but pneumonia occurred significantly more frequently in hares infected with the B.6 genotype compared to hares infected with the B.12 genotype.",
author = "M.G.J. Koene and Rijks, {Jolianne M.} and Miriam Maas and R.C. Ruuls and M.Y. Engelsma and {van Tulden}, P.W. and Marja Kik and Jooske IJzer and Daan Notermans and {de Vries}, Maaike and Ewout Fanoy and Roan Pijnacker and Spierenburg, {Marcel A.H.} and Herjan Bavelaar and Hanneke Berkhout and Sanjay Sankatsing and Rob Diepersloot and Kerstin Myrtennas and Malin Granberg and Mats Forsman and H.I.J. Roest and Andrea Gr{\"o}ne",
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Koene, MGJ, Rijks, JM, Maas, M, Ruuls, RC, Engelsma, MY, van Tulden, PW, Kik, M, IJzer, J, Notermans, D, de Vries, M, Fanoy, E, Pijnacker, R, Spierenburg, MAH, Bavelaar, H, Berkhout, H, Sankatsing, S, Diepersloot, R, Myrtennas, K, Granberg, M, Forsman, M, Roest, HIJ & Gröne, A 2019, 'Phylogeographic distribution of human and hare Francisella tularenses susp. holarctica strains in the Netherlands and its pathology in European brown hares (Lepus europaeus)', Frontiers in Cellular and Infection Microbiology, vol. 9, 11. https://doi.org/10.3389/fcimb.2019.00011

Phylogeographic distribution of human and hare Francisella tularenses susp. holarctica strains in the Netherlands and its pathology in European brown hares (Lepus europaeus). / Koene, M.G.J.; Rijks, Jolianne M.; Maas, Miriam; Ruuls, R.C.; Engelsma, M.Y.; van Tulden, P.W.; Kik, Marja; IJzer, Jooske; Notermans, Daan; de Vries, Maaike; Fanoy, Ewout; Pijnacker, Roan; Spierenburg, Marcel A.H.; Bavelaar, Herjan; Berkhout, Hanneke; Sankatsing, Sanjay; Diepersloot, Rob; Myrtennas, Kerstin; Granberg, Malin; Forsman, Mats; Roest, H.I.J.; Gröne, Andrea.

In: Frontiers in Cellular and Infection Microbiology, Vol. 9, 11, 11.02.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Phylogeographic distribution of human and hare Francisella tularenses susp. holarctica strains in the Netherlands and its pathology in European brown hares (Lepus europaeus)

AU - Koene, M.G.J.

AU - Rijks, Jolianne M.

AU - Maas, Miriam

AU - Ruuls, R.C.

AU - Engelsma, M.Y.

AU - van Tulden, P.W.

AU - Kik, Marja

AU - IJzer, Jooske

AU - Notermans, Daan

AU - de Vries, Maaike

AU - Fanoy, Ewout

AU - Pijnacker, Roan

AU - Spierenburg, Marcel A.H.

AU - Bavelaar, Herjan

AU - Berkhout, Hanneke

AU - Sankatsing, Sanjay

AU - Diepersloot, Rob

AU - Myrtennas, Kerstin

AU - Granberg, Malin

AU - Forsman, Mats

AU - Roest, H.I.J.

AU - Gröne, Andrea

N1 - Manuscript ID: 429749

PY - 2019/2/11

Y1 - 2019/2/11

N2 - Sequence-based typing of Francisella tularensis has led to insights in the evolutionary developments of tularemia. In Europe, two major basal clades of F. tularensis subsp. holarctica exist, with a distinct geographical distribution. Basal clade B.6 is primarily found in Western Europe, while basal clade B.12 occurs predominantly in the central and eastern parts of Europe. There are indications that tularemia is geographically expanding and that strains from the two clades might differ in pathogenicity, with basal clade B.6 strains being potentially more virulent than basal clade B.12. This study provides information on genotypes detected in the Netherlands during 2011–2017. Data are presented for seven autochthonous human cases and for 29 European brown hares (Lepus europaeus) with laboratory confirmed tularemia. Associated disease patterns are described for 25 European brown hares which underwent post-mortem examination. The basal clades B.6 and B.12 are present both in humans and in European brown hares in the Netherlands, with a patchy geographical distribution. For both genotypes the main pathological findings in hares associated with tularemia were severe (sub)acute necrotizing hepatitis and splenitis as well as necrotizing lesions and hemorrhages in several other organs. Pneumonia was significantly more common in the B.6 than in the B.12 cases. In conclusion, the two major basal clades present in different parts in Europe are both present in the Netherlands. In hares found dead, both genotypes were associated with severe acute disease affecting multiple organs. Hepatitis and splenitis were common pathological findings in hares infected with either genotype, but pneumonia occurred significantly more frequently in hares infected with the B.6 genotype compared to hares infected with the B.12 genotype.

AB - Sequence-based typing of Francisella tularensis has led to insights in the evolutionary developments of tularemia. In Europe, two major basal clades of F. tularensis subsp. holarctica exist, with a distinct geographical distribution. Basal clade B.6 is primarily found in Western Europe, while basal clade B.12 occurs predominantly in the central and eastern parts of Europe. There are indications that tularemia is geographically expanding and that strains from the two clades might differ in pathogenicity, with basal clade B.6 strains being potentially more virulent than basal clade B.12. This study provides information on genotypes detected in the Netherlands during 2011–2017. Data are presented for seven autochthonous human cases and for 29 European brown hares (Lepus europaeus) with laboratory confirmed tularemia. Associated disease patterns are described for 25 European brown hares which underwent post-mortem examination. The basal clades B.6 and B.12 are present both in humans and in European brown hares in the Netherlands, with a patchy geographical distribution. For both genotypes the main pathological findings in hares associated with tularemia were severe (sub)acute necrotizing hepatitis and splenitis as well as necrotizing lesions and hemorrhages in several other organs. Pneumonia was significantly more common in the B.6 than in the B.12 cases. In conclusion, the two major basal clades present in different parts in Europe are both present in the Netherlands. In hares found dead, both genotypes were associated with severe acute disease affecting multiple organs. Hepatitis and splenitis were common pathological findings in hares infected with either genotype, but pneumonia occurred significantly more frequently in hares infected with the B.6 genotype compared to hares infected with the B.12 genotype.

U2 - 10.3389/fcimb.2019.00011

DO - 10.3389/fcimb.2019.00011

M3 - Article

VL - 9

JO - Frontiers in Cellular and Infection Microbiology

T2 - Frontiers in Cellular and Infection Microbiology

JF - Frontiers in Cellular and Infection Microbiology

SN - 2235-2988

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ER -