Abstract
We constructed several multilocus DNA sequence datasets to assess the phylogenetic
diversity of insecticolous fusaria, especially focusing on those housed at the Agricultural
Research Service Collection of Entomopathogenic Fungi (ARSEF), and to aid molecular
identifications of unknowns via the FUSARIUM-ID and Fusarium MLST online databases and
analysis packages. Analyses of a 190-taxon, two-locus dataset, which included 159 isolates from
insects, indicated that: (i) insect-associated fusaria were nested within 10 species complexes
spanning the phylogenetic breadth of Fusarium, (ii) novel, putatively unnamed insecticolous
species were nested within 8/10 species complexes and (iii) Latin binomials could be applied
with confidence to only 18/58 phylogenetically distinct fusaria associated with pest insects.
Phylogenetic analyses of an 82-taxon, three-locus dataset nearly fully resolved evolutionary
relationships among the 10 clades containing insecticolous fusaria. Multilocus typing of isolates
within four species complexes identified surprisingly high genetic diversity in that 63/65 of the
fusaria typed represented newly discovered haplotypes. The DNA sequence data, together with
corrected ABI sequence chromatograms and alignments, have been uploaded to the following
websites dedicated to identifying fusaria: FUSARIUM-ID (http://isolate.fusariumdb.org) at
| Original language | English |
|---|---|
| Pages (from-to) | 427-445 |
| Journal | Mycologia |
| Volume | 104 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 |
Keywords
- fujikuroi species complex
- rna-polymerase-ii
- gene genealogies
- contact-lens
- fungi
- evolution
- solani
- recognition
- model
- rpb2