Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana

Stefan P.W. De Vries, Moses Vurayai, Mark Holmes, Srishti Gupta, Michael Bateman, David Goldfarb, Duncan J. Maskell, Maitshwarelo Ignatius Matsheka, Andrew J. Grant*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Campylobacter spp. are a leading cause of bacterial enteritis worldwide, including countries in Africa, and have been identified by the World Health Organisation (WHO) as one of the high priority antimicrobial resistant pathogens. However, at present there is little knowledge on the prevalence, molecular epidemiology or antimicrobial susceptibility of Campylobacter spp. isolates in Botswana, both in patients and in the zoonotic context. Some data indicate that ~14% of diarrhoeal disease cases in a paediatric setting can be ascribed to Campylobacter spp., urging the need for the magnitude of Campylobacter-associated diarrhoea to be established. In this survey, we have characterised the genomic diversity of Campylobacter spp. circulating in Botswana isolated from cases of diarrhoeal disease in humans (n = 20) and from those that colonised commercial broiler (n = 35) and free-range (n = 35) chickens. Phylogeny showed that the Campylobacter spp. isolated from the different poultry and human sources were highly related, suggesting that zoonotic transmission has likely occurred. We found that for Campylobacter spp. isolated from humans, broilers and free-range chickens, 52% was positive for tetO, 47% for gyrA-T86I, 72% for blaOXA-61, with 27% carrying all three resistance determinants. No 23S mutations conferring macrolide resistance were detected in this survey. In summary, our study provides insight into Campylobacter spp. in poultry reservoirs and in diarrhoeal patients, and the relevance for treatment regimens in Botswana.
Original languageEnglish
Article numbere0194481
JournalPLoS ONE
Volume13
Issue number3
DOIs
Publication statusPublished - 1 Mar 2018

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Botswana
Poultry
Campylobacter
antibiotic resistance
Chickens
chickens
Pediatrics
phylogeny
Macrolides
Pathogens
Health
Zoonoses
poultry
bacterial enteritis
anti-infective agents
broiler chickens
macrolides
molecular epidemiology
Molecular Epidemiology
Enteritis

Cite this

De Vries, S. P. W., Vurayai, M., Holmes, M., Gupta, S., Bateman, M., Goldfarb, D., ... Grant, A. J. (2018). Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana. PLoS ONE, 13(3), [e0194481]. https://doi.org/10.1371/journal.pone.0194481
De Vries, Stefan P.W. ; Vurayai, Moses ; Holmes, Mark ; Gupta, Srishti ; Bateman, Michael ; Goldfarb, David ; Maskell, Duncan J. ; Matsheka, Maitshwarelo Ignatius ; Grant, Andrew J. / Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana. In: PLoS ONE. 2018 ; Vol. 13, No. 3.
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title = "Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana",
abstract = "Campylobacter spp. are a leading cause of bacterial enteritis worldwide, including countries in Africa, and have been identified by the World Health Organisation (WHO) as one of the high priority antimicrobial resistant pathogens. However, at present there is little knowledge on the prevalence, molecular epidemiology or antimicrobial susceptibility of Campylobacter spp. isolates in Botswana, both in patients and in the zoonotic context. Some data indicate that ~14{\%} of diarrhoeal disease cases in a paediatric setting can be ascribed to Campylobacter spp., urging the need for the magnitude of Campylobacter-associated diarrhoea to be established. In this survey, we have characterised the genomic diversity of Campylobacter spp. circulating in Botswana isolated from cases of diarrhoeal disease in humans (n = 20) and from those that colonised commercial broiler (n = 35) and free-range (n = 35) chickens. Phylogeny showed that the Campylobacter spp. isolated from the different poultry and human sources were highly related, suggesting that zoonotic transmission has likely occurred. We found that for Campylobacter spp. isolated from humans, broilers and free-range chickens, 52{\%} was positive for tetO, 47{\%} for gyrA-T86I, 72{\%} for blaOXA-61, with 27{\%} carrying all three resistance determinants. No 23S mutations conferring macrolide resistance were detected in this survey. In summary, our study provides insight into Campylobacter spp. in poultry reservoirs and in diarrhoeal patients, and the relevance for treatment regimens in Botswana.",
author = "{De Vries}, {Stefan P.W.} and Moses Vurayai and Mark Holmes and Srishti Gupta and Michael Bateman and David Goldfarb and Maskell, {Duncan J.} and Matsheka, {Maitshwarelo Ignatius} and Grant, {Andrew J.}",
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De Vries, SPW, Vurayai, M, Holmes, M, Gupta, S, Bateman, M, Goldfarb, D, Maskell, DJ, Matsheka, MI & Grant, AJ 2018, 'Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana', PLoS ONE, vol. 13, no. 3, e0194481. https://doi.org/10.1371/journal.pone.0194481

Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana. / De Vries, Stefan P.W.; Vurayai, Moses; Holmes, Mark; Gupta, Srishti; Bateman, Michael; Goldfarb, David; Maskell, Duncan J.; Matsheka, Maitshwarelo Ignatius; Grant, Andrew J.

In: PLoS ONE, Vol. 13, No. 3, e0194481, 01.03.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana

AU - De Vries, Stefan P.W.

AU - Vurayai, Moses

AU - Holmes, Mark

AU - Gupta, Srishti

AU - Bateman, Michael

AU - Goldfarb, David

AU - Maskell, Duncan J.

AU - Matsheka, Maitshwarelo Ignatius

AU - Grant, Andrew J.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Campylobacter spp. are a leading cause of bacterial enteritis worldwide, including countries in Africa, and have been identified by the World Health Organisation (WHO) as one of the high priority antimicrobial resistant pathogens. However, at present there is little knowledge on the prevalence, molecular epidemiology or antimicrobial susceptibility of Campylobacter spp. isolates in Botswana, both in patients and in the zoonotic context. Some data indicate that ~14% of diarrhoeal disease cases in a paediatric setting can be ascribed to Campylobacter spp., urging the need for the magnitude of Campylobacter-associated diarrhoea to be established. In this survey, we have characterised the genomic diversity of Campylobacter spp. circulating in Botswana isolated from cases of diarrhoeal disease in humans (n = 20) and from those that colonised commercial broiler (n = 35) and free-range (n = 35) chickens. Phylogeny showed that the Campylobacter spp. isolated from the different poultry and human sources were highly related, suggesting that zoonotic transmission has likely occurred. We found that for Campylobacter spp. isolated from humans, broilers and free-range chickens, 52% was positive for tetO, 47% for gyrA-T86I, 72% for blaOXA-61, with 27% carrying all three resistance determinants. No 23S mutations conferring macrolide resistance were detected in this survey. In summary, our study provides insight into Campylobacter spp. in poultry reservoirs and in diarrhoeal patients, and the relevance for treatment regimens in Botswana.

AB - Campylobacter spp. are a leading cause of bacterial enteritis worldwide, including countries in Africa, and have been identified by the World Health Organisation (WHO) as one of the high priority antimicrobial resistant pathogens. However, at present there is little knowledge on the prevalence, molecular epidemiology or antimicrobial susceptibility of Campylobacter spp. isolates in Botswana, both in patients and in the zoonotic context. Some data indicate that ~14% of diarrhoeal disease cases in a paediatric setting can be ascribed to Campylobacter spp., urging the need for the magnitude of Campylobacter-associated diarrhoea to be established. In this survey, we have characterised the genomic diversity of Campylobacter spp. circulating in Botswana isolated from cases of diarrhoeal disease in humans (n = 20) and from those that colonised commercial broiler (n = 35) and free-range (n = 35) chickens. Phylogeny showed that the Campylobacter spp. isolated from the different poultry and human sources were highly related, suggesting that zoonotic transmission has likely occurred. We found that for Campylobacter spp. isolated from humans, broilers and free-range chickens, 52% was positive for tetO, 47% for gyrA-T86I, 72% for blaOXA-61, with 27% carrying all three resistance determinants. No 23S mutations conferring macrolide resistance were detected in this survey. In summary, our study provides insight into Campylobacter spp. in poultry reservoirs and in diarrhoeal patients, and the relevance for treatment regimens in Botswana.

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DO - 10.1371/journal.pone.0194481

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