Photosynthetic acclimation in relation to nitrogen allocation in cucumber leaves in response to changes in irradiance

G. Trouwborst, S.W. Hogewoning, J. Harbinson, W. van Ieperen

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

Leaves deep in canopies can suddenly be exposed to increased irradiances following e.g. gap formation in forests or pruning in crops. Studies on the acclimation of photosynthesis to increased irradiance have mainly focused on the changes in photosynthetic capacity (Amax), although actual irradiance often remains below saturating level. We investigated the effect of changes in irradiance on the photosynthesis irradiance response and on nitrogen allocation in fully grown leaves of Cucumis sativus. Leaves that fully developed under low (50 µmol m-2 s-1) or moderate (200 µmol m-2 s-1) irradiance were subsequently exposed to, respectively, moderate (LM-leaves) or low (ML-leaves) irradiance or kept at constant irradiance level (LL- and MM-leaves). Acclimation of photosynthesis occurred within 7 days with final Amax highest in MM-leaves, lowest in LL-leaves and intermediate in ML- and LM-leaves, whereas full acclimation of thylakoid processes underlying photosystem II (PSII) efficiency and non-photochemical quenching occurred in ML- and LM-leaves. Dark respiration correlated with irradiance level, but not with Amax. Light-limited quantum efficiency was similar in all leaves. The increase in photosynthesis at moderate irradiance in LM-leaves was primarily driven by nitrogen import, and nitrogen remained allocated in a similar ratio to Rubisco and bioenergetics, while allocation to light harvesting relatively decreased. A contrary response of nitrogen was associated with the decrease in photosynthesis in ML-leaves. Net assimilation of LM-leaves under moderate irradiance remained lower than in MM-leaves, revealing the importance of photosynthetic acclimation during the leaf developmental phase for crop productivity in scenarios with realistic, moderate fluctuations in irradiance that leaves can be exposed to
LanguageEnglish
Pages157-169
JournalPhysiologia Plantarum
Volume142
Issue number2
DOIs
Publication statusPublished - 2011

Fingerprint

Cucumis sativus
Acclimatization
Photosynthesis
cucumbers
acclimation
Nitrogen
nitrogen
leaves
Ribulose-Bisphosphate Carboxylase
Light
Thylakoids
Photosystem II Protein Complex
photosynthesis
Energy Metabolism
Respiration
light intensity

Keywords

  • mesophyll diffusion conductance
  • chlorophyll-a fluorescence
  • season canopy gap
  • light-acclimation
  • leaf anatomy
  • temperature response
  • c-3 photosynthesis
  • electron-transport
  • biochemical-model
  • growth irradiance

Cite this

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title = "Photosynthetic acclimation in relation to nitrogen allocation in cucumber leaves in response to changes in irradiance",
abstract = "Leaves deep in canopies can suddenly be exposed to increased irradiances following e.g. gap formation in forests or pruning in crops. Studies on the acclimation of photosynthesis to increased irradiance have mainly focused on the changes in photosynthetic capacity (Amax), although actual irradiance often remains below saturating level. We investigated the effect of changes in irradiance on the photosynthesis irradiance response and on nitrogen allocation in fully grown leaves of Cucumis sativus. Leaves that fully developed under low (50 µmol m-2 s-1) or moderate (200 µmol m-2 s-1) irradiance were subsequently exposed to, respectively, moderate (LM-leaves) or low (ML-leaves) irradiance or kept at constant irradiance level (LL- and MM-leaves). Acclimation of photosynthesis occurred within 7 days with final Amax highest in MM-leaves, lowest in LL-leaves and intermediate in ML- and LM-leaves, whereas full acclimation of thylakoid processes underlying photosystem II (PSII) efficiency and non-photochemical quenching occurred in ML- and LM-leaves. Dark respiration correlated with irradiance level, but not with Amax. Light-limited quantum efficiency was similar in all leaves. The increase in photosynthesis at moderate irradiance in LM-leaves was primarily driven by nitrogen import, and nitrogen remained allocated in a similar ratio to Rubisco and bioenergetics, while allocation to light harvesting relatively decreased. A contrary response of nitrogen was associated with the decrease in photosynthesis in ML-leaves. Net assimilation of LM-leaves under moderate irradiance remained lower than in MM-leaves, revealing the importance of photosynthetic acclimation during the leaf developmental phase for crop productivity in scenarios with realistic, moderate fluctuations in irradiance that leaves can be exposed to",
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Photosynthetic acclimation in relation to nitrogen allocation in cucumber leaves in response to changes in irradiance. / Trouwborst, G.; Hogewoning, S.W.; Harbinson, J.; van Ieperen, W.

In: Physiologia Plantarum, Vol. 142, No. 2, 2011, p. 157-169.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Photosynthetic acclimation in relation to nitrogen allocation in cucumber leaves in response to changes in irradiance

AU - Trouwborst, G.

AU - Hogewoning, S.W.

AU - Harbinson, J.

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KW - mesophyll diffusion conductance

KW - chlorophyll-a fluorescence

KW - season canopy gap

KW - light-acclimation

KW - leaf anatomy

KW - temperature response

KW - c-3 photosynthesis

KW - electron-transport

KW - biochemical-model

KW - growth irradiance

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DO - 10.1111/j.1399-3054.2011.01456.x

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T2 - Physiologia Plantarum

JF - Physiologia Plantarum

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