Photoacoustic measurement of lutein in biological matrix

D.D. Bicanic, S. Luterotti, M. Becucci, V. Fogliano, P. Versloot

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Photoacoustic (PA) spectroscopy was applied for the first time to quantify lutein in a complex biological matrix. Standard addition of lutein to a biological low-lutein matrix was used for the calibration. The PA signal was found linearly proportional (R > 0.98) to lutein concentration up to 0.3% (w/w). The dynamic range of concentrations extends to 1% (w/w) lutein. For a given experimental set-up the responsivity of PA detector within the range of linearity was estimated to 1.1 mV/1% lutein. Precision of repeated analyses is good with average RSD values of 4 and 5% for blanks and spiked samples, respectively. The analytical parameters indicate that the PA method is fast and sensitive enough for quantification of lutein in supplementary drugs and in the lutein-rich foods.
Original languageEnglish
Pages (from-to)825-828
JournalJournal de Physique IV France
Volume125
DOIs
Publication statusPublished - 2005

Keywords

  • performance liquid-chromatography
  • thermal lens detection
  • trans-beta-carotene
  • ultrasensitive determination
  • vegetable-oils
  • hplc-tls

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