Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins.

M. Blaauw, J.C. Knol, A. Kortholt, J. Roelofs, A. Ruchira, M. Postma, A.J.W.G. Visser, P.J.M. van Haastert

Research output: Contribution to journalArticleAcademicpeer-review

43 Citations (Scopus)


Retinal phosducin is known to sequester transducin Gbetagamma, thereby modulating transducin activity. Phos ducin is a member of a family of phosducin-like proteins (PhLP) found in eukaryotes. Phylogeny of 33 phosducin-like proteins from metazoa, plants and lower eukaryotes identified three distinct groups named phosducin-I-III. We discovered three phlp genes in Dictyostelium, each encoding a phosducin-like protein of a different group. Disruption of the phlp1 gene strongly impaired G-protein signalling, apparently due to mislocalization of Gbetagamma in phlp1-null cells. GFP-Gbeta and GFP-Ggamma are membrane associated in wild-type cells, but cytosolic in phlp1-null cells. Phlp2 disruption is lethal due to a synchronous collapse of the cells after 16-17 cell divisions. Phlp3 disruptants show no abnormal phenotype. These results establish a role for phosducin-like proteins in facilitating folding, localization or function of proteins, in addition to modulating G-protein signalling.
Original languageEnglish
Pages (from-to)5047-5057
JournalThe EMBO Journal
Issue number19
Publication statusPublished - 2003


  • g-beta-gamma
  • heterotrimeric g-proteins
  • subunit function
  • regulator
  • complex
  • chemotaxis
  • transducin
  • cells
  • phlp
  • gradients

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