TY - JOUR
T1 - Phenotyping flag leaf nitrogen content in rice using a three-band spectral index
AU - Wang, Jian Jun
AU - Li, Zhikang
AU - Jin, Xiuliang
AU - Liang, Guohua
AU - Struik, Paul C.
AU - Gu, Junfei
AU - Zhou, Yong
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Accurate, rapid and non-destructive measurements of rice flag leaf nitrogen content (LNC) are crucial for geneticists and breeders. To help design a less expensive, non-destructive, real-time LNC sensor, we developed a Three-Band Difference Ratio (TBDR) spectral index, TBDR (R 755 , R 513 , R 508 ). This spectral index could accurately and rapidly estimate rice LNC in a population of chromosome segment substitution lines with small variation in LNC. The model estimating LNC was validated using a leave-one-out cross-validation technique; the achieved root mean square error was 0.13% and the relative error was 4.74%. In comparison with SPAD-502plus chlorophyll meter readings and commonly used spectral indices, including GreenSeeker- and Crop Circle-based indices, TBDR (R 755 , R 513 , R 508 ) produced higher accuracy in LNC estimation.
AB - Accurate, rapid and non-destructive measurements of rice flag leaf nitrogen content (LNC) are crucial for geneticists and breeders. To help design a less expensive, non-destructive, real-time LNC sensor, we developed a Three-Band Difference Ratio (TBDR) spectral index, TBDR (R 755 , R 513 , R 508 ). This spectral index could accurately and rapidly estimate rice LNC in a population of chromosome segment substitution lines with small variation in LNC. The model estimating LNC was validated using a leave-one-out cross-validation technique; the achieved root mean square error was 0.13% and the relative error was 4.74%. In comparison with SPAD-502plus chlorophyll meter readings and commonly used spectral indices, including GreenSeeker- and Crop Circle-based indices, TBDR (R 755 , R 513 , R 508 ) produced higher accuracy in LNC estimation.
KW - Chromosome segment substitution lines
KW - Oryza sativa
KW - SPAD-502plus chlorophyll meter
KW - Spectral reflectance
U2 - 10.1016/j.compag.2019.04.042
DO - 10.1016/j.compag.2019.04.042
M3 - Article
AN - SCOPUS:85064933313
SN - 0168-1699
VL - 162
SP - 475
EP - 481
JO - Computers and Electronics in Agriculture
JF - Computers and Electronics in Agriculture
ER -