Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches

R.J. Siezen, V.A. Tzeneva, A. Castioni, M.W.W. Wels, H.T. Phan, J.L.W. Rademaker, M.J.C. Starrenburg, M. Kleerebezem, D. Molenaar, J.E.T. van Hylckama Vlieg

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174 Citations (Scopus)

Abstract

Lactobacillus plantarum is a ubiquitous microorganism that is able to colonize several ecological niches, including vegetables, meat, dairy substrates and the gastro-intestinal tract. An extensive phenotypic and genomic diversity analysis was conducted to elucidate the molecular basis of the high flexibility and versatility of this species. First, 185 isolates from diverse environments were phenotypically characterized by evaluating their fermentation and growth characteristics. Strains clustered largely together within their particular food niche, but human fecal isolates were scattered throughout the food clusters, suggesting that they originate from the food eaten by the individuals. Based on distinct phenotypic profiles, 24 strains were selected and, together with a further 18 strains from an earlier low-resolution study, their genomic diversity was evaluated by comparative genome hybridization against the reference genome of L. plantarum WCFS1. Over 2000 genes were identified that constitute the core genome of the L. plantarum species, including 121 unique L. plantarum-marker genes that have not been found in other lactic acid bacteria. Over 50 genes unique for the reference strain WCFS1 were identified that were absent in the other L. plantarum strains. Strains of the L. plantarum subspecies argentoratensis were found to lack a common set of 24 genes, organized in seven gene clusters/operons, supporting their classification as a separate subspecies. The results provide a detailed view on phenotypic and genomic diversity of L. plantarum and lead to a better comprehension of niche adaptation and functionality of the organism
Original languageEnglish
Pages (from-to)758-773
JournalEnvironmental Microbiology
Volume12
Issue number3
DOIs
Publication statusPublished - 2010

Keywords

  • lactic-acid bacteria
  • horizontal gene-transfer
  • streptococcus-thermophilus
  • starter cultures
  • sequence
  • identification
  • paraplantarum
  • evolution
  • pcr
  • differentiation

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  • Datasets

    Batch wt vs hyper +_- pABA Normal int_Filtered fg-bg2

    Mars, A. (Creator), Faijes, M. (Creator), Molenaar, D. (Creator), de Vos, R. C. (Creator), Klaus, S. M. (Creator), Hanson, A. D. (Creator), de Vos, W. (Creator) & Smid, E. (Creator), NIZO, 31 May 2008

    Dataset

    Analysis of the role of CggR in Lactobacillus plantarum NC8 and WCFS1 by transcriptome analysis

    Rud, I. (Creator), Naterstad, K. (Creator), Bongers, R. S. (Creator), Molenaar, D. (Creator), Kleerebezem, M. (Creator) & Axelsson, L. (Creator), NIZO, 1 Jan 2010

    Dataset

    Heme_k2_Filtered bckg correct trans-norm

    Serrano, L. M. (Creator), Molenaar, D. (Creator), Teusink, B. (Creator), de Vos, W. (Creator) & Smid, E. (Creator), TI Food and Nutrition, 16 Aug 2007

    Dataset

    Cite this

    Siezen, R. J., Tzeneva, V. A., Castioni, A., Wels, M. W. W., Phan, H. T., Rademaker, J. L. W., Starrenburg, M. J. C., Kleerebezem, M., Molenaar, D., & van Hylckama Vlieg, J. E. T. (2010). Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches. Environmental Microbiology, 12(3), 758-773. https://doi.org/10.1111/j.1462-2920.2009.02119.x