Phenotypic and genetic diversity of the species Lactobacillus rhamnosus

C.D. Ceapa

Research output: Thesisinternal PhD, WU

Abstract

The thesis explores the diversity of Lactobacillus rhamnosus, a species from which strains are studied for their anti-inflammatory, anti-allergic, and diarrhea preventing effects. The work combines observations on the behavior of the bacteria in a simplified laboratory setting (use of carbohydrates, immune modulation effects, anti-pathogenic effects) with genomic information obtained by sequencing, with the aim to pinpoint genes that could be relevant for bacterial survival and metabolic capacities. Phenotypic and genotypic profiling analyses congruently revealed that carbohydrate metabolism and transport is essential for this species’ adaptation to the environment. Genotype–phenotype correlation analysis enabled us to predict and then experimentally verify genes responsible for the utilization of L-Sorbose, L-Fucose α-D-Methyl Glycoside.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Kleerebezem, Michiel, Promotor
  • Knol, Jan, Promotor
  • Lambert, J., Promotor, External person
Award date12 Jan 2016
Place of PublicationWageningen
Publisher
Print ISBNs9789462576285
Publication statusPublished - 2016

Fingerprint

Lactobacillus rhamnosus
sorbose
genetic variation
fucose
carbohydrate metabolism
glycosides
diarrhea
genes
carbohydrates
genomics
bacteria

Keywords

  • fermentation products
  • probiotics
  • intestinal microorganisms
  • lactic acid bacteria
  • strains
  • medicinal properties
  • genomics
  • nutrition and health

Cite this

Ceapa, C. D. (2016). Phenotypic and genetic diversity of the species Lactobacillus rhamnosus. Wageningen: Wageningen University.
Ceapa, C.D.. / Phenotypic and genetic diversity of the species Lactobacillus rhamnosus. Wageningen : Wageningen University, 2016. 195 p.
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abstract = "The thesis explores the diversity of Lactobacillus rhamnosus, a species from which strains are studied for their anti-inflammatory, anti-allergic, and diarrhea preventing effects. The work combines observations on the behavior of the bacteria in a simplified laboratory setting (use of carbohydrates, immune modulation effects, anti-pathogenic effects) with genomic information obtained by sequencing, with the aim to pinpoint genes that could be relevant for bacterial survival and metabolic capacities. Phenotypic and genotypic profiling analyses congruently revealed that carbohydrate metabolism and transport is essential for this species’ adaptation to the environment. Genotype–phenotype correlation analysis enabled us to predict and then experimentally verify genes responsible for the utilization of L-Sorbose, L-Fucose α-D-Methyl Glycoside.",
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author = "C.D. Ceapa",
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Ceapa, CD 2016, 'Phenotypic and genetic diversity of the species Lactobacillus rhamnosus', Doctor of Philosophy, Wageningen University, Wageningen.

Phenotypic and genetic diversity of the species Lactobacillus rhamnosus. / Ceapa, C.D.

Wageningen : Wageningen University, 2016. 195 p.

Research output: Thesisinternal PhD, WU

TY - THES

T1 - Phenotypic and genetic diversity of the species Lactobacillus rhamnosus

AU - Ceapa, C.D.

N1 - WU thesis 6258

PY - 2016

Y1 - 2016

N2 - The thesis explores the diversity of Lactobacillus rhamnosus, a species from which strains are studied for their anti-inflammatory, anti-allergic, and diarrhea preventing effects. The work combines observations on the behavior of the bacteria in a simplified laboratory setting (use of carbohydrates, immune modulation effects, anti-pathogenic effects) with genomic information obtained by sequencing, with the aim to pinpoint genes that could be relevant for bacterial survival and metabolic capacities. Phenotypic and genotypic profiling analyses congruently revealed that carbohydrate metabolism and transport is essential for this species’ adaptation to the environment. Genotype–phenotype correlation analysis enabled us to predict and then experimentally verify genes responsible for the utilization of L-Sorbose, L-Fucose α-D-Methyl Glycoside.

AB - The thesis explores the diversity of Lactobacillus rhamnosus, a species from which strains are studied for their anti-inflammatory, anti-allergic, and diarrhea preventing effects. The work combines observations on the behavior of the bacteria in a simplified laboratory setting (use of carbohydrates, immune modulation effects, anti-pathogenic effects) with genomic information obtained by sequencing, with the aim to pinpoint genes that could be relevant for bacterial survival and metabolic capacities. Phenotypic and genotypic profiling analyses congruently revealed that carbohydrate metabolism and transport is essential for this species’ adaptation to the environment. Genotype–phenotype correlation analysis enabled us to predict and then experimentally verify genes responsible for the utilization of L-Sorbose, L-Fucose α-D-Methyl Glycoside.

KW - fermentation products

KW - probiotics

KW - intestinal microorganisms

KW - lactic acid bacteria

KW - strains

KW - medicinal properties

KW - genomics

KW - nutrition and health

KW - fermentatieproducten

KW - probiotica

KW - darmmicro-organismen

KW - melkzuurbacteriën

KW - stammen (biologisch)

KW - medicinale eigenschappen

KW - genomica

KW - voeding en gezondheid

M3 - internal PhD, WU

SN - 9789462576285

PB - Wageningen University

CY - Wageningen

ER -

Ceapa CD. Phenotypic and genetic diversity of the species Lactobacillus rhamnosus. Wageningen: Wageningen University, 2016. 195 p.