Phase behavior of phosphatidylglycerol in spinach thylakoid membranes as revealed by 31P-NMR

S.K.B. Krumova, C. Dijkema, P. de Waard, H. van As, G. Garab, H. van Amerongen

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)

Abstract

Non-bilayer lipids account for about half of the total lipid content in chloroplast thylakoid membranes. This lends high propensity of the thylakoid lipid mixture to participate in different phases which might be functionally required. It is for instance known that the chloroplast enzyme violaxanthin de-epoxidase (VDE) requires a non-bilayer phase for proper functioning in vitro but direct evidence for the presence of non-bilayer lipid structures in thylakoid membranes under physiological conditions is still missing. In this work, we used phosphatidylglycerol (PG) as an intrinsic bulk lipid label for 31P-NMR studies to monitor lipid phases of thylakoid membranes. We show that in intact thylakoid membranes the characteristic lamellar signal is observed only below 20 °C. But at the same time an isotropic phase is present, which becomes even dominant between 14 and 28 °C despite the presence of fully functional large membrane sheets that are capable of generating and maintaining a transmembrane electric field. Tris-washed membranes show a similar behavior but the lamellar phase is present up to higher temperatures. Thus, our data show that the location of the phospholipids is not restricted to the bilayer phase and that the lamellar phase co-exists with a non-bilayer isotropic phase.
Original languageEnglish
Pages (from-to)997-1003
JournalBiochimica et Biophysica Acta. Biomembranes
Volume1778
Issue number4
DOIs
Publication statusPublished - 2008

Fingerprint

Phosphatidylglycerols
Thylakoids
Spinacia oleracea
Phase behavior
Nuclear magnetic resonance
Membranes
Lipids
Chloroplasts
Labels
Phospholipids
Electric fields
Temperature
Enzymes

Keywords

  • violaxanthin de-epoxidase
  • photosystem-ii
  • photosynthetic membranes
  • structural flexibility
  • lipid polymorphism
  • nonbilayer lipids
  • protein complexes
  • deepoxidation
  • temperature
  • particles

Cite this

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title = "Phase behavior of phosphatidylglycerol in spinach thylakoid membranes as revealed by 31P-NMR",
abstract = "Non-bilayer lipids account for about half of the total lipid content in chloroplast thylakoid membranes. This lends high propensity of the thylakoid lipid mixture to participate in different phases which might be functionally required. It is for instance known that the chloroplast enzyme violaxanthin de-epoxidase (VDE) requires a non-bilayer phase for proper functioning in vitro but direct evidence for the presence of non-bilayer lipid structures in thylakoid membranes under physiological conditions is still missing. In this work, we used phosphatidylglycerol (PG) as an intrinsic bulk lipid label for 31P-NMR studies to monitor lipid phases of thylakoid membranes. We show that in intact thylakoid membranes the characteristic lamellar signal is observed only below 20 °C. But at the same time an isotropic phase is present, which becomes even dominant between 14 and 28 °C despite the presence of fully functional large membrane sheets that are capable of generating and maintaining a transmembrane electric field. Tris-washed membranes show a similar behavior but the lamellar phase is present up to higher temperatures. Thus, our data show that the location of the phospholipids is not restricted to the bilayer phase and that the lamellar phase co-exists with a non-bilayer isotropic phase.",
keywords = "violaxanthin de-epoxidase, photosystem-ii, photosynthetic membranes, structural flexibility, lipid polymorphism, nonbilayer lipids, protein complexes, deepoxidation, temperature, particles",
author = "S.K.B. Krumova and C. Dijkema and {de Waard}, P. and {van As}, H. and G. Garab and {van Amerongen}, H.",
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Phase behavior of phosphatidylglycerol in spinach thylakoid membranes as revealed by 31P-NMR. / Krumova, S.K.B.; Dijkema, C.; de Waard, P.; van As, H.; Garab, G.; van Amerongen, H.

In: Biochimica et Biophysica Acta. Biomembranes, Vol. 1778, No. 4, 2008, p. 997-1003.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Phase behavior of phosphatidylglycerol in spinach thylakoid membranes as revealed by 31P-NMR

AU - Krumova, S.K.B.

AU - Dijkema, C.

AU - de Waard, P.

AU - van As, H.

AU - Garab, G.

AU - van Amerongen, H.

PY - 2008

Y1 - 2008

N2 - Non-bilayer lipids account for about half of the total lipid content in chloroplast thylakoid membranes. This lends high propensity of the thylakoid lipid mixture to participate in different phases which might be functionally required. It is for instance known that the chloroplast enzyme violaxanthin de-epoxidase (VDE) requires a non-bilayer phase for proper functioning in vitro but direct evidence for the presence of non-bilayer lipid structures in thylakoid membranes under physiological conditions is still missing. In this work, we used phosphatidylglycerol (PG) as an intrinsic bulk lipid label for 31P-NMR studies to monitor lipid phases of thylakoid membranes. We show that in intact thylakoid membranes the characteristic lamellar signal is observed only below 20 °C. But at the same time an isotropic phase is present, which becomes even dominant between 14 and 28 °C despite the presence of fully functional large membrane sheets that are capable of generating and maintaining a transmembrane electric field. Tris-washed membranes show a similar behavior but the lamellar phase is present up to higher temperatures. Thus, our data show that the location of the phospholipids is not restricted to the bilayer phase and that the lamellar phase co-exists with a non-bilayer isotropic phase.

AB - Non-bilayer lipids account for about half of the total lipid content in chloroplast thylakoid membranes. This lends high propensity of the thylakoid lipid mixture to participate in different phases which might be functionally required. It is for instance known that the chloroplast enzyme violaxanthin de-epoxidase (VDE) requires a non-bilayer phase for proper functioning in vitro but direct evidence for the presence of non-bilayer lipid structures in thylakoid membranes under physiological conditions is still missing. In this work, we used phosphatidylglycerol (PG) as an intrinsic bulk lipid label for 31P-NMR studies to monitor lipid phases of thylakoid membranes. We show that in intact thylakoid membranes the characteristic lamellar signal is observed only below 20 °C. But at the same time an isotropic phase is present, which becomes even dominant between 14 and 28 °C despite the presence of fully functional large membrane sheets that are capable of generating and maintaining a transmembrane electric field. Tris-washed membranes show a similar behavior but the lamellar phase is present up to higher temperatures. Thus, our data show that the location of the phospholipids is not restricted to the bilayer phase and that the lamellar phase co-exists with a non-bilayer isotropic phase.

KW - violaxanthin de-epoxidase

KW - photosystem-ii

KW - photosynthetic membranes

KW - structural flexibility

KW - lipid polymorphism

KW - nonbilayer lipids

KW - protein complexes

KW - deepoxidation

KW - temperature

KW - particles

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DO - 10.1016/j.bbamem.2008.01.004

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SN - 0005-2736

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