Pharmacology of stomoxytachykinin receptor depends on second messenger system

J. Poels; R.J. Nachman; K.E. Akerman; H.B. Oonk; F. Guerrero; A. de Loof; A.E. Janecka; H. Torfs; J. Vanden Broeck

doi:10.1016/j.peptides.2004.07.015

Pharmacology of stomoxytachykinin receptor depends on second messenger system

J. Poels, R.J. Nachman, K.E. Akerman, H.B. Oonk, F. Guerrero, A. de Loof, A.E. Janecka, H. Torfs, J. Vanden Broeck

Wageningen Livestock Research

Research output: Contribution to journal › Article › Academic › peer-review

18 Citations (Scopus)

Abstract

STKR is a neurokinin receptor derived from the stable fly, Stomoxys calcitrans. Insect tachykinin-related peptides, also referred to as ¿insectatachykinins¿, produce dose-dependent calcium and cyclic AMP responses in cultured Drosophila melanogaster Schneider 2 (S2) cells that were stably transfected with the cloned STKR cDNA. Pronounced differences in pharmacology were observed between agonist-induced calcium and cyclic AMP responses. The results indicate that the pharmacological properties of STKR depend on its coupling to a unique second messenger system. Therefore, a model postulating the existence of multiple active receptor conformations is proposed. This article presents the first evidence that an insect peptide receptor with dual coupling properties to second messenger systems can display agonist-dependent functional differences.

Original language	English
Pages (from-to)	109-114
Journal	Peptides
Volume	26
Issue number	1
DOIs	https://doi.org/10.1016/j.peptides.2004.07.015
Publication status	Published - 2005

Keywords

protein-coupled receptors
tachykinin-like peptides
drosophila-melanogaster
thyrotropin receptor
insect neuropeptides
nk1 receptor
agonist
expression
homology
family

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10.1016/j.peptides.2004.07.015

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@article{e04c3442c7bd48728312e3f68d52a1ab,

title = "Pharmacology of stomoxytachykinin receptor depends on second messenger system",

abstract = "STKR is a neurokinin receptor derived from the stable fly, Stomoxys calcitrans. Insect tachykinin-related peptides, also referred to as ¿insectatachykinins¿, produce dose-dependent calcium and cyclic AMP responses in cultured Drosophila melanogaster Schneider 2 (S2) cells that were stably transfected with the cloned STKR cDNA. Pronounced differences in pharmacology were observed between agonist-induced calcium and cyclic AMP responses. The results indicate that the pharmacological properties of STKR depend on its coupling to a unique second messenger system. Therefore, a model postulating the existence of multiple active receptor conformations is proposed. This article presents the first evidence that an insect peptide receptor with dual coupling properties to second messenger systems can display agonist-dependent functional differences.",

keywords = "protein-coupled receptors, tachykinin-like peptides, drosophila-melanogaster, thyrotropin receptor, insect neuropeptides, nk1 receptor, agonist, expression, homology, family",

author = "J. Poels and R.J. Nachman and K.E. Akerman and H.B. Oonk and F. Guerrero and {de Loof}, A. and A.E. Janecka and H. Torfs and {Vanden Broeck}, J.",

note = "Times Cited: 3",

year = "2005",

doi = "10.1016/j.peptides.2004.07.015",

language = "English",

volume = "26",

pages = "109--114",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier",

number = "1",

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TY - JOUR

T1 - Pharmacology of stomoxytachykinin receptor depends on second messenger system

AU - Poels, J.

AU - Nachman, R.J.

AU - Akerman, K.E.

AU - Oonk, H.B.

AU - Guerrero, F.

AU - de Loof, A.

AU - Janecka, A.E.

AU - Torfs, H.

AU - Vanden Broeck, J.

N1 - Times Cited: 3

PY - 2005

Y1 - 2005

N2 - STKR is a neurokinin receptor derived from the stable fly, Stomoxys calcitrans. Insect tachykinin-related peptides, also referred to as ¿insectatachykinins¿, produce dose-dependent calcium and cyclic AMP responses in cultured Drosophila melanogaster Schneider 2 (S2) cells that were stably transfected with the cloned STKR cDNA. Pronounced differences in pharmacology were observed between agonist-induced calcium and cyclic AMP responses. The results indicate that the pharmacological properties of STKR depend on its coupling to a unique second messenger system. Therefore, a model postulating the existence of multiple active receptor conformations is proposed. This article presents the first evidence that an insect peptide receptor with dual coupling properties to second messenger systems can display agonist-dependent functional differences.

AB - STKR is a neurokinin receptor derived from the stable fly, Stomoxys calcitrans. Insect tachykinin-related peptides, also referred to as ¿insectatachykinins¿, produce dose-dependent calcium and cyclic AMP responses in cultured Drosophila melanogaster Schneider 2 (S2) cells that were stably transfected with the cloned STKR cDNA. Pronounced differences in pharmacology were observed between agonist-induced calcium and cyclic AMP responses. The results indicate that the pharmacological properties of STKR depend on its coupling to a unique second messenger system. Therefore, a model postulating the existence of multiple active receptor conformations is proposed. This article presents the first evidence that an insect peptide receptor with dual coupling properties to second messenger systems can display agonist-dependent functional differences.

KW - protein-coupled receptors

KW - tachykinin-like peptides

KW - drosophila-melanogaster

KW - thyrotropin receptor

KW - insect neuropeptides

KW - nk1 receptor

KW - agonist

KW - expression

KW - homology

KW - family

U2 - 10.1016/j.peptides.2004.07.015

DO - 10.1016/j.peptides.2004.07.015

M3 - Article

SN - 0196-9781

VL - 26

SP - 109

EP - 114

JO - Peptides

JF - Peptides

IS - 1

ER -

Pharmacology of stomoxytachykinin receptor depends on second messenger system

Abstract

Keywords

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