Phage therapy reduces Campylobacter jejuni colonization in broilers

J.A. Wagenaar, M.A.P. van Bergen, M.A. Mueller, T.M. Wassenaar, R.M. Carlton

Research output: Contribution to journalArticleAcademicpeer-review

193 Citations (Scopus)

Abstract

The effect of phage therapy in the control of Campylobacter jejuni colonization in young broilers, either as a preventive or a therapeutic measure, was tested. A prevention group was infected with C. jejuni at day 4 of a 10-day phage treatment. A therapeutic group was phage treated for 6 days, starting 5 days after C. jejuni colonization of the broilers had been established. Treatment was monitored by enumerating Campylobacter colony forming units (CFU) and phage plaque forming units (PFU) from caecal content. Counts were compared with control birds not receiving phage therapy. A clear 3 log decline in C. jejuni counts was initially observed in the therapeutic group, however, after 5 days bacterial counts stabilized at a level 1 log lower than that of the control group. Colonization of C. jejuni in the prevention group was delayed by the treatment and after an initial 2 log reduction, colonization stabilized within a week at levels comparable to the therapeutic group. The CFU and PFU counts displayed opposing highs and lows over time, indicative of alternating shifts in amplification of bacteria and phages. There were no adverse health effects from the phage treatment. Two different phages were combined as therapeutic treatment of Campylobacter positive chickens challenged at the age approaching broiler harvest. This again resulted in a significant decrease in Campylobacter colonization. We conclude that phage treatment is a promising alternative for reducing C. jejuni colonization in broilers.
Original languageEnglish
Pages (from-to)275-283
JournalVeterinary Microbiology
Volume109
Issue number3-4
DOIs
Publication statusPublished - 2005

Keywords

  • quantitative risk-assessment
  • bacteriophage therapy
  • escherichia-coli
  • competitive-exclusion
  • united-states
  • chicken skin
  • infection
  • poultry
  • agents
  • contamination

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