Phage display-selected single chain antibodies confer high levels of resistance against Tomato spotted wilt virus

M.W. Prins, H. Lohuis, A. Schots, R.W. Goldbach

Research output: Contribution to journalArticleAcademicpeer-review

32 Citations (Scopus)

Abstract

Rational design of antibodies targeting essential viral proteins can complement the palette of antiviral resistance strategies. Here, stable and high expression of single-chain monoclonal antibodies targeting the nucleoprotein of the economically important plant virus Tomato spotted wilt virus, a protein that is involved in multiple steps in the viral infection cycle, is reported. High cytoplasmic expression levels of three selected phage display-derived anti-viral single-chain antibodies were established. Of these antibodies, two led to high levels of resistance against this plant virus. Protoplast experiments provided evidence that the two resistance-conferring antibodies may have a different mode of action and could be combined for higher durability of resistance in the field
Original languageEnglish
Pages (from-to)2107-2113
JournalJournal of General Virology
Volume86
Issue number7
DOIs
Publication statusPublished - 2005

Keywords

  • transgenic tobacco
  • gene-sequences
  • rna-polymerase
  • plant cytosol
  • fv-antibody
  • expression
  • protein
  • fragments
  • cells
  • tospoviruses

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