Peripheral inflammation-induced changes in songbird brain gene expression: 3’ mRNA transcriptomic approach

Nithya Kuttiyarthu Veetil, Haniel Cedraz de Oliveira, Mercedes Gomez-Samblas, Daniel Divín, Balraj Melepat, Eleni Voukali, Zuzana Świderská, Tereza Krajzingrová, Martin Těšický, Ferris Jung, Vladimír Beneš, Ole Madsen, Michal Vinkler*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Species-specific neural inflammation can be induced by profound immune signalling from periphery to brain. Recent advances in transcriptomics offer cost-effective approaches to study this regulation. In a population of captive zebra finch (Taeniopygia guttata), we compare the differential gene expression patterns in lipopolysaccharide (LPS)-triggered peripheral inflammation revealed by RNA-seq and QuantSeq. The RNA-seq approach identified more differentially expressed genes but failed to detect any inflammatory markers. In contrast, QuantSeq results identified specific expression changes in the genes regulating inflammation. Next, we adopted QuantSeq to relate peripheral and brain transcriptomes. We identified subtle changes in the brain gene expression during the peripheral inflammation (e.g. up-regulation in AVD-like and ACOD1 expression) and detected co-structure between the peripheral and brain inflammation. Our results suggest benefits of the 3′end transcriptomics for association studies between peripheral and neural inflammation in genetically heterogeneous models and identify potential targets for the future brain research in birds.

Original languageEnglish
Article number105106
JournalDevelopmental and Comparative Immunology
Volume151
DOIs
Publication statusPublished - Feb 2024

Keywords

  • Avian cytokine
  • Differential gene expression
  • Neurogenic inflammation
  • Neuroimmune interaction
  • Peripheral immunity
  • Transcriptome

Fingerprint

Dive into the research topics of 'Peripheral inflammation-induced changes in songbird brain gene expression: 3’ mRNA transcriptomic approach'. Together they form a unique fingerprint.

Cite this