Peripheral Blood Mononuclear Cells (Pbmc) As A Source To Detect Markers Of Intake Of Diets With Unbalanced Proportion Of Macronutrients

R. Diaz-Rua, P. Oliver, E.M. van Schothorst, A. Caimari, J. Keijer, A. Palou

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Background and objectives: Unbalanced proportion in diet macronutrients is associated to alterations in body weight and/or metabolic parameters, and can be related to different pathologies. In order to find markers of intake of unbalance diets we analysed gene expression in peripheral blood mononuclear cells (PBMC). PBMC constitute an easy obtainable biological material which use for nutrition studies is increasing as they can reproduce gene expression of other internal tissues and reflect different pathological status. Methods: Wistar rats were fed for 4 months with a control (70% Kcal from carbohydrates), a high-fat (HF; 60% Kcal from fats) or a high-protein (HP; 45% Kcal from proteins) diet in isocaloric amounts (pair-feeding). PBMC were obtained and gene expression studied by microarray analysis. Results: Our microarray data showed that 113 and 323 genes of a total of 21,530 probes changed as a result of the intake of a HF and a HP diet, respectively (Student’s t test, p
Original languageEnglish
Title of host publicationAbstracts 20th International Congress of Nutrition
Place of PublicationBasel
PublisherKarger
Pages338-338
Volume63
ISBN (Print)9783318025163
DOIs
Publication statusPublished - 2013
Event20th International Congress of Nutrition, Granada, Spain -
Duration: 15 Sep 201320 Sep 2013

Conference

Conference20th International Congress of Nutrition, Granada, Spain
Period15/09/1320/09/13

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Blood Cells
Diet
Gene Expression
Fats
Microarray Analysis
Wistar Rats
Proteins
Body Weight
Carbohydrates
Pathology
Students
Genes

Cite this

Diaz-Rua, R., Oliver, P., van Schothorst, E. M., Caimari, A., Keijer, J., & Palou, A. (2013). Peripheral Blood Mononuclear Cells (Pbmc) As A Source To Detect Markers Of Intake Of Diets With Unbalanced Proportion Of Macronutrients. In Abstracts 20th International Congress of Nutrition (Vol. 63, pp. 338-338). Basel: Karger. https://doi.org/10.1159/isbn.978-3-318-02516-3
Diaz-Rua, R. ; Oliver, P. ; van Schothorst, E.M. ; Caimari, A. ; Keijer, J. ; Palou, A. / Peripheral Blood Mononuclear Cells (Pbmc) As A Source To Detect Markers Of Intake Of Diets With Unbalanced Proportion Of Macronutrients. Abstracts 20th International Congress of Nutrition. Vol. 63 Basel : Karger, 2013. pp. 338-338
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Diaz-Rua, R, Oliver, P, van Schothorst, EM, Caimari, A, Keijer, J & Palou, A 2013, Peripheral Blood Mononuclear Cells (Pbmc) As A Source To Detect Markers Of Intake Of Diets With Unbalanced Proportion Of Macronutrients. in Abstracts 20th International Congress of Nutrition. vol. 63, Karger, Basel, pp. 338-338, 20th International Congress of Nutrition, Granada, Spain, 15/09/13. https://doi.org/10.1159/isbn.978-3-318-02516-3

Peripheral Blood Mononuclear Cells (Pbmc) As A Source To Detect Markers Of Intake Of Diets With Unbalanced Proportion Of Macronutrients. / Diaz-Rua, R.; Oliver, P.; van Schothorst, E.M.; Caimari, A.; Keijer, J.; Palou, A.

Abstracts 20th International Congress of Nutrition. Vol. 63 Basel : Karger, 2013. p. 338-338.

Research output: Chapter in Book/Report/Conference proceedingAbstract

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Diaz-Rua R, Oliver P, van Schothorst EM, Caimari A, Keijer J, Palou A. Peripheral Blood Mononuclear Cells (Pbmc) As A Source To Detect Markers Of Intake Of Diets With Unbalanced Proportion Of Macronutrients. In Abstracts 20th International Congress of Nutrition. Vol. 63. Basel: Karger. 2013. p. 338-338 https://doi.org/10.1159/isbn.978-3-318-02516-3