Performance of Chlorella sorokiniana under simulated extreme winter conditions

M. Cuaresma, M.F. Buffing, M.G.J. Janssen, C.V. Lobato, R.H. Wijffels

Research output: Contribution to journalArticleAcademicpeer-review

37 Citations (Scopus)

Abstract

High annual microalgae productivities can only be achieved if solar light is efficiently used through the different seasons. During winter the productivity is low because of the light and temperature conditions. The productivity and photosynthetic efficiency of Chlorella sorokiniana were assessed under the worst-case scenario found during winter time in Huelva, south of Spain. The maximum light intensity (800 µmol photons m-2 s-1) and temperature (20°C) during winter were simulated in a lab-scale photobioreactor with a short light-path of 14 mm. Chemostat conditions were applied and the results were compared with a temperature-controlled situation at 38°C (optimal growth temperature for C. sorokiniana). When temperature was optimal the highest productivity was found at a dilution rate of 0.18 h-1 (P v¿=¿0.28 g Kg-1 h-1), and the biomass yield on light energy was high (Y x,E¿=¿1.2 g¿mol-1 photons supplied). However, at suboptimal temperature, the specific growth rate of C. sorokiniana was surprisingly low, not being able to support continuous operation at a dilution rate higher than 0.02 h-1. The slow metabolism under suboptimal temperature resulted in a decline of the light energy requirements of the cells. Consequently, the maximum winter irradiance was experienced as excessive, leading to a low photosynthetic efficiency and productivity (Y x,E¿=¿0.5 g mol-1 photons supplied, P v¿=¿0.1 g Kg-1 h-1). At suboptimal temperature a higher carotenoid-to-chlorophyll ratio was observed indicating the activation of light-dissipating processes. We conclude that temperature control and/or light dilution during winter time will enhance the productivity.
Original languageEnglish
Pages (from-to)693-699
JournalJournal of Applied Phycology
Volume24
Issue number4
DOIs
Publication statusPublished - 2012

Fingerprint

Chlorella sorokiniana
winter
productivity
temperature
dilution
carotenoid
microalgae
energy requirements
light intensity
specific growth rate
irradiance
energy
carotenoids
chlorophyll
metabolism
Spain

Keywords

  • flat-plate photobioreactor
  • photosynthetic efficiency
  • spirulina-platensis
  • light-path
  • outdoor cultures
  • temperature
  • productivity
  • growth
  • microalgae
  • density

Cite this

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title = "Performance of Chlorella sorokiniana under simulated extreme winter conditions",
abstract = "High annual microalgae productivities can only be achieved if solar light is efficiently used through the different seasons. During winter the productivity is low because of the light and temperature conditions. The productivity and photosynthetic efficiency of Chlorella sorokiniana were assessed under the worst-case scenario found during winter time in Huelva, south of Spain. The maximum light intensity (800 µmol photons m-2 s-1) and temperature (20°C) during winter were simulated in a lab-scale photobioreactor with a short light-path of 14 mm. Chemostat conditions were applied and the results were compared with a temperature-controlled situation at 38°C (optimal growth temperature for C. sorokiniana). When temperature was optimal the highest productivity was found at a dilution rate of 0.18 h-1 (P v¿=¿0.28 g Kg-1 h-1), and the biomass yield on light energy was high (Y x,E¿=¿1.2 g¿mol-1 photons supplied). However, at suboptimal temperature, the specific growth rate of C. sorokiniana was surprisingly low, not being able to support continuous operation at a dilution rate higher than 0.02 h-1. The slow metabolism under suboptimal temperature resulted in a decline of the light energy requirements of the cells. Consequently, the maximum winter irradiance was experienced as excessive, leading to a low photosynthetic efficiency and productivity (Y x,E¿=¿0.5 g mol-1 photons supplied, P v¿=¿0.1 g Kg-1 h-1). At suboptimal temperature a higher carotenoid-to-chlorophyll ratio was observed indicating the activation of light-dissipating processes. We conclude that temperature control and/or light dilution during winter time will enhance the productivity.",
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author = "M. Cuaresma and M.F. Buffing and M.G.J. Janssen and C.V. Lobato and R.H. Wijffels",
year = "2012",
doi = "10.1007/s10811-011-9687-y",
language = "English",
volume = "24",
pages = "693--699",
journal = "Journal of Applied Phycology",
issn = "0921-8971",
publisher = "Springer Verlag",
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Performance of Chlorella sorokiniana under simulated extreme winter conditions. / Cuaresma, M.; Buffing, M.F.; Janssen, M.G.J.; Lobato, C.V.; Wijffels, R.H.

In: Journal of Applied Phycology, Vol. 24, No. 4, 2012, p. 693-699.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Performance of Chlorella sorokiniana under simulated extreme winter conditions

AU - Cuaresma, M.

AU - Buffing, M.F.

AU - Janssen, M.G.J.

AU - Lobato, C.V.

AU - Wijffels, R.H.

PY - 2012

Y1 - 2012

N2 - High annual microalgae productivities can only be achieved if solar light is efficiently used through the different seasons. During winter the productivity is low because of the light and temperature conditions. The productivity and photosynthetic efficiency of Chlorella sorokiniana were assessed under the worst-case scenario found during winter time in Huelva, south of Spain. The maximum light intensity (800 µmol photons m-2 s-1) and temperature (20°C) during winter were simulated in a lab-scale photobioreactor with a short light-path of 14 mm. Chemostat conditions were applied and the results were compared with a temperature-controlled situation at 38°C (optimal growth temperature for C. sorokiniana). When temperature was optimal the highest productivity was found at a dilution rate of 0.18 h-1 (P v¿=¿0.28 g Kg-1 h-1), and the biomass yield on light energy was high (Y x,E¿=¿1.2 g¿mol-1 photons supplied). However, at suboptimal temperature, the specific growth rate of C. sorokiniana was surprisingly low, not being able to support continuous operation at a dilution rate higher than 0.02 h-1. The slow metabolism under suboptimal temperature resulted in a decline of the light energy requirements of the cells. Consequently, the maximum winter irradiance was experienced as excessive, leading to a low photosynthetic efficiency and productivity (Y x,E¿=¿0.5 g mol-1 photons supplied, P v¿=¿0.1 g Kg-1 h-1). At suboptimal temperature a higher carotenoid-to-chlorophyll ratio was observed indicating the activation of light-dissipating processes. We conclude that temperature control and/or light dilution during winter time will enhance the productivity.

AB - High annual microalgae productivities can only be achieved if solar light is efficiently used through the different seasons. During winter the productivity is low because of the light and temperature conditions. The productivity and photosynthetic efficiency of Chlorella sorokiniana were assessed under the worst-case scenario found during winter time in Huelva, south of Spain. The maximum light intensity (800 µmol photons m-2 s-1) and temperature (20°C) during winter were simulated in a lab-scale photobioreactor with a short light-path of 14 mm. Chemostat conditions were applied and the results were compared with a temperature-controlled situation at 38°C (optimal growth temperature for C. sorokiniana). When temperature was optimal the highest productivity was found at a dilution rate of 0.18 h-1 (P v¿=¿0.28 g Kg-1 h-1), and the biomass yield on light energy was high (Y x,E¿=¿1.2 g¿mol-1 photons supplied). However, at suboptimal temperature, the specific growth rate of C. sorokiniana was surprisingly low, not being able to support continuous operation at a dilution rate higher than 0.02 h-1. The slow metabolism under suboptimal temperature resulted in a decline of the light energy requirements of the cells. Consequently, the maximum winter irradiance was experienced as excessive, leading to a low photosynthetic efficiency and productivity (Y x,E¿=¿0.5 g mol-1 photons supplied, P v¿=¿0.1 g Kg-1 h-1). At suboptimal temperature a higher carotenoid-to-chlorophyll ratio was observed indicating the activation of light-dissipating processes. We conclude that temperature control and/or light dilution during winter time will enhance the productivity.

KW - flat-plate photobioreactor

KW - photosynthetic efficiency

KW - spirulina-platensis

KW - light-path

KW - outdoor cultures

KW - temperature

KW - productivity

KW - growth

KW - microalgae

KW - density

U2 - 10.1007/s10811-011-9687-y

DO - 10.1007/s10811-011-9687-y

M3 - Article

VL - 24

SP - 693

EP - 699

JO - Journal of Applied Phycology

JF - Journal of Applied Phycology

SN - 0921-8971

IS - 4

ER -