Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines

F.A. Krens, J.G. Schaart, R. Groenwold, A.E.J. Walraven, T. Hesselink, J.T.N.M. Thissen

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Introduction of sustainable scab resistance in elite apple cultivars is of high importance for apple cultivation when aiming at reducing the use of chemical crop protectants. Genetic modification (GM) allows the rapid introduction of resistance genes directly into high quality apple cultivars. Resistance genes can be derived from apple itself but genetic modification also opens up the possibility to use other, non-host resistance genes. A prerequisite for application is the long-term performance and stability of the gene annex trait in the field. For this study, we produced and selected a series of transgenic apple lines of two cultivars, i.e. ‘Elstar’ and ‘Gala’ in which the barley hordothionin gene (hth) was introduced. After multiplication, the GM hth-lines, non-GM susceptible and resistant controls and GM non-hth controls were planted in a random block design in a field trial in 40 replicates. Scab resistance was monitored after artificial inoculation (first year) and after natural infection (subsequent years). After the trial period, the level of expression of the hth gene was checked by quantitative RT-PCR. Four of the six GM hth apple lines proved to be significantly less susceptible to apple scab and this trait was found to be stable for the entire 4-year period. Hth expression at the mRNA level was also stable
Original languageEnglish
Pages (from-to)1113-1123
JournalTransgenic Research
Volume20
Issue number5
DOIs
Publication statusPublished - 2011

Fingerprint

Malus
Hordeum
apples
barley
genetically modified organisms
genetic engineering
Genes
genes
cultivars
Hordeum vulgare hordothionin protein
Venturia inaequalis
field experimentation
reverse transcriptase polymerase chain reaction
Gene Expression
Polymerase Chain Reaction
gene expression
Messenger RNA
crops
Infection

Keywords

  • receptor-like genes
  • scab resistance
  • venturia-inaequalis
  • expression
  • thionins
  • vf
  • transformation
  • agrobacterium
  • plants
  • wheat

Cite this

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title = "Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines",
abstract = "Introduction of sustainable scab resistance in elite apple cultivars is of high importance for apple cultivation when aiming at reducing the use of chemical crop protectants. Genetic modification (GM) allows the rapid introduction of resistance genes directly into high quality apple cultivars. Resistance genes can be derived from apple itself but genetic modification also opens up the possibility to use other, non-host resistance genes. A prerequisite for application is the long-term performance and stability of the gene annex trait in the field. For this study, we produced and selected a series of transgenic apple lines of two cultivars, i.e. ‘Elstar’ and ‘Gala’ in which the barley hordothionin gene (hth) was introduced. After multiplication, the GM hth-lines, non-GM susceptible and resistant controls and GM non-hth controls were planted in a random block design in a field trial in 40 replicates. Scab resistance was monitored after artificial inoculation (first year) and after natural infection (subsequent years). After the trial period, the level of expression of the hth gene was checked by quantitative RT-PCR. Four of the six GM hth apple lines proved to be significantly less susceptible to apple scab and this trait was found to be stable for the entire 4-year period. Hth expression at the mRNA level was also stable",
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author = "F.A. Krens and J.G. Schaart and R. Groenwold and A.E.J. Walraven and T. Hesselink and J.T.N.M. Thissen",
year = "2011",
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Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines. / Krens, F.A.; Schaart, J.G.; Groenwold, R.; Walraven, A.E.J.; Hesselink, T.; Thissen, J.T.N.M.

In: Transgenic Research, Vol. 20, No. 5, 2011, p. 1113-1123.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines

AU - Krens, F.A.

AU - Schaart, J.G.

AU - Groenwold, R.

AU - Walraven, A.E.J.

AU - Hesselink, T.

AU - Thissen, J.T.N.M.

PY - 2011

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N2 - Introduction of sustainable scab resistance in elite apple cultivars is of high importance for apple cultivation when aiming at reducing the use of chemical crop protectants. Genetic modification (GM) allows the rapid introduction of resistance genes directly into high quality apple cultivars. Resistance genes can be derived from apple itself but genetic modification also opens up the possibility to use other, non-host resistance genes. A prerequisite for application is the long-term performance and stability of the gene annex trait in the field. For this study, we produced and selected a series of transgenic apple lines of two cultivars, i.e. ‘Elstar’ and ‘Gala’ in which the barley hordothionin gene (hth) was introduced. After multiplication, the GM hth-lines, non-GM susceptible and resistant controls and GM non-hth controls were planted in a random block design in a field trial in 40 replicates. Scab resistance was monitored after artificial inoculation (first year) and after natural infection (subsequent years). After the trial period, the level of expression of the hth gene was checked by quantitative RT-PCR. Four of the six GM hth apple lines proved to be significantly less susceptible to apple scab and this trait was found to be stable for the entire 4-year period. Hth expression at the mRNA level was also stable

AB - Introduction of sustainable scab resistance in elite apple cultivars is of high importance for apple cultivation when aiming at reducing the use of chemical crop protectants. Genetic modification (GM) allows the rapid introduction of resistance genes directly into high quality apple cultivars. Resistance genes can be derived from apple itself but genetic modification also opens up the possibility to use other, non-host resistance genes. A prerequisite for application is the long-term performance and stability of the gene annex trait in the field. For this study, we produced and selected a series of transgenic apple lines of two cultivars, i.e. ‘Elstar’ and ‘Gala’ in which the barley hordothionin gene (hth) was introduced. After multiplication, the GM hth-lines, non-GM susceptible and resistant controls and GM non-hth controls were planted in a random block design in a field trial in 40 replicates. Scab resistance was monitored after artificial inoculation (first year) and after natural infection (subsequent years). After the trial period, the level of expression of the hth gene was checked by quantitative RT-PCR. Four of the six GM hth apple lines proved to be significantly less susceptible to apple scab and this trait was found to be stable for the entire 4-year period. Hth expression at the mRNA level was also stable

KW - receptor-like genes

KW - scab resistance

KW - venturia-inaequalis

KW - expression

KW - thionins

KW - vf

KW - transformation

KW - agrobacterium

KW - plants

KW - wheat

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JO - Transgenic Research

JF - Transgenic Research

SN - 0962-8819

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