Abstract
| Language | English |
|---|---|
| Pages | 46-52 |
| Journal | International Dairy Journal |
| Volume | 46 |
| DOIs | |
| Publication status | Published - 2015 |
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Keywords
- enzyme inhibitory peptides
- globule-membrane proteome
- somatic-cell count
- casein
- proteolysis
- hydrolysate
- lactation
- antibacterial
- colostrum
- casecidin
Cite this
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Peptidomic analysis of healthy and subclinically mastitic bovine milk. / Guerrero, A.; Dallas, D.C.; Contreras, S.; Bhandari, A.; Canovas, A.; Islas-Trejo, A.; Medrano, J.F.; Parker, E.A.; Wang, M.; Hettinga, K.A.; Chee, S.; German, J.B.; Barile, D.; Lebrilla, C.B.
In: International Dairy Journal, Vol. 46, 2015, p. 46-52.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Peptidomic analysis of healthy and subclinically mastitic bovine milk
AU - Guerrero, A.
AU - Dallas, D.C.
AU - Contreras, S.
AU - Bhandari, A.
AU - Canovas, A.
AU - Islas-Trejo, A.
AU - Medrano, J.F.
AU - Parker, E.A.
AU - Wang, M.
AU - Hettinga, K.A.
AU - Chee, S.
AU - German, J.B.
AU - Barile, D.
AU - Lebrilla, C.B.
PY - 2015
Y1 - 2015
N2 - A variety of proteases release hundreds of endogenous peptide fragments from intact bovine milk proteins. Mass spectrometry-based peptidomics allows for high throughput sequence assignment of a large number of these peptides. Mastitis is known to result in increased protease activity in the mammary gland. Therefore, we hypothesized that subclinically mastitic milks would contain higher concentrations of released peptides. In this work, milks were sampled from three cows and, for each, one healthy and one subclinically mastitic teat were sampled for milk. Peptides were analyzed by nano-liquid chromatography quadrupole time of flight tandem mass spectrometry and identified with database searching. In total, 682 peptides were identified. The total number of released peptides increased 146% from healthy to subclinically mastitic milks (p <0.05), and the total abundance of released peptides also increased significantly (p <0.05). Bioinformatic analysis of enzyme cleavage revealed increases in activity of cathepsin D and elastase (p <0.05) with subclinical mastitis.
AB - A variety of proteases release hundreds of endogenous peptide fragments from intact bovine milk proteins. Mass spectrometry-based peptidomics allows for high throughput sequence assignment of a large number of these peptides. Mastitis is known to result in increased protease activity in the mammary gland. Therefore, we hypothesized that subclinically mastitic milks would contain higher concentrations of released peptides. In this work, milks were sampled from three cows and, for each, one healthy and one subclinically mastitic teat were sampled for milk. Peptides were analyzed by nano-liquid chromatography quadrupole time of flight tandem mass spectrometry and identified with database searching. In total, 682 peptides were identified. The total number of released peptides increased 146% from healthy to subclinically mastitic milks (p <0.05), and the total abundance of released peptides also increased significantly (p <0.05). Bioinformatic analysis of enzyme cleavage revealed increases in activity of cathepsin D and elastase (p <0.05) with subclinical mastitis.
KW - enzyme inhibitory peptides
KW - globule-membrane proteome
KW - somatic-cell count
KW - casein
KW - proteolysis
KW - hydrolysate
KW - lactation
KW - antibacterial
KW - colostrum
KW - casecidin
U2 - 10.1016/j.idairyj.2014.09.006
DO - 10.1016/j.idairyj.2014.09.006
M3 - Article
VL - 46
SP - 46
EP - 52
JO - International Dairy Journal
T2 - International Dairy Journal
JF - International Dairy Journal
SN - 0958-6946
ER -