Peptidomic analysis of healthy and subclinically mastitic bovine milk

A. Guerrero, D.C. Dallas, S. Contreras, A. Bhandari, A. Canovas, A. Islas-Trejo, J.F. Medrano, E.A. Parker, M. Wang, K.A. Hettinga, S. Chee, J.B. German, D. Barile, C.B. Lebrilla

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

A variety of proteases release hundreds of endogenous peptide fragments from intact bovine milk proteins. Mass spectrometry-based peptidomics allows for high throughput sequence assignment of a large number of these peptides. Mastitis is known to result in increased protease activity in the mammary gland. Therefore, we hypothesized that subclinically mastitic milks would contain higher concentrations of released peptides. In this work, milks were sampled from three cows and, for each, one healthy and one subclinically mastitic teat were sampled for milk. Peptides were analyzed by nano-liquid chromatography quadrupole time of flight tandem mass spectrometry and identified with database searching. In total, 682 peptides were identified. The total number of released peptides increased 146% from healthy to subclinically mastitic milks (p <0.05), and the total abundance of released peptides also increased significantly (p <0.05). Bioinformatic analysis of enzyme cleavage revealed increases in activity of cathepsin D and elastase (p <0.05) with subclinical mastitis.
LanguageEnglish
Pages46-52
JournalInternational Dairy Journal
Volume46
DOIs
Publication statusPublished - 2015

Fingerprint

Milk
peptides
milk
Peptides
Mastitis
Peptide Hydrolases
mastitis
proteinases
Cathepsin D
Peptide Fragments
cathepsin D
Pancreatic Elastase
elastase
Tandem Mass Spectrometry
Computational Biology
teats
Mass Spectrometry
bioinformatics
mass spectrometry
cows

Keywords

  • enzyme inhibitory peptides
  • globule-membrane proteome
  • somatic-cell count
  • casein
  • proteolysis
  • hydrolysate
  • lactation
  • antibacterial
  • colostrum
  • casecidin

Cite this

Guerrero, A., Dallas, D. C., Contreras, S., Bhandari, A., Canovas, A., Islas-Trejo, A., ... Lebrilla, C. B. (2015). Peptidomic analysis of healthy and subclinically mastitic bovine milk. International Dairy Journal, 46, 46-52. https://doi.org/10.1016/j.idairyj.2014.09.006
Guerrero, A. ; Dallas, D.C. ; Contreras, S. ; Bhandari, A. ; Canovas, A. ; Islas-Trejo, A. ; Medrano, J.F. ; Parker, E.A. ; Wang, M. ; Hettinga, K.A. ; Chee, S. ; German, J.B. ; Barile, D. ; Lebrilla, C.B. / Peptidomic analysis of healthy and subclinically mastitic bovine milk. In: International Dairy Journal. 2015 ; Vol. 46. pp. 46-52.
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abstract = "A variety of proteases release hundreds of endogenous peptide fragments from intact bovine milk proteins. Mass spectrometry-based peptidomics allows for high throughput sequence assignment of a large number of these peptides. Mastitis is known to result in increased protease activity in the mammary gland. Therefore, we hypothesized that subclinically mastitic milks would contain higher concentrations of released peptides. In this work, milks were sampled from three cows and, for each, one healthy and one subclinically mastitic teat were sampled for milk. Peptides were analyzed by nano-liquid chromatography quadrupole time of flight tandem mass spectrometry and identified with database searching. In total, 682 peptides were identified. The total number of released peptides increased 146{\%} from healthy to subclinically mastitic milks (p <0.05), and the total abundance of released peptides also increased significantly (p <0.05). Bioinformatic analysis of enzyme cleavage revealed increases in activity of cathepsin D and elastase (p <0.05) with subclinical mastitis.",
keywords = "enzyme inhibitory peptides, globule-membrane proteome, somatic-cell count, casein, proteolysis, hydrolysate, lactation, antibacterial, colostrum, casecidin",
author = "A. Guerrero and D.C. Dallas and S. Contreras and A. Bhandari and A. Canovas and A. Islas-Trejo and J.F. Medrano and E.A. Parker and M. Wang and K.A. Hettinga and S. Chee and J.B. German and D. Barile and C.B. Lebrilla",
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Guerrero, A, Dallas, DC, Contreras, S, Bhandari, A, Canovas, A, Islas-Trejo, A, Medrano, JF, Parker, EA, Wang, M, Hettinga, KA, Chee, S, German, JB, Barile, D & Lebrilla, CB 2015, 'Peptidomic analysis of healthy and subclinically mastitic bovine milk', International Dairy Journal, vol. 46, pp. 46-52. https://doi.org/10.1016/j.idairyj.2014.09.006

Peptidomic analysis of healthy and subclinically mastitic bovine milk. / Guerrero, A.; Dallas, D.C.; Contreras, S.; Bhandari, A.; Canovas, A.; Islas-Trejo, A.; Medrano, J.F.; Parker, E.A.; Wang, M.; Hettinga, K.A.; Chee, S.; German, J.B.; Barile, D.; Lebrilla, C.B.

In: International Dairy Journal, Vol. 46, 2015, p. 46-52.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Peptidomic analysis of healthy and subclinically mastitic bovine milk

AU - Guerrero, A.

AU - Dallas, D.C.

AU - Contreras, S.

AU - Bhandari, A.

AU - Canovas, A.

AU - Islas-Trejo, A.

AU - Medrano, J.F.

AU - Parker, E.A.

AU - Wang, M.

AU - Hettinga, K.A.

AU - Chee, S.

AU - German, J.B.

AU - Barile, D.

AU - Lebrilla, C.B.

PY - 2015

Y1 - 2015

N2 - A variety of proteases release hundreds of endogenous peptide fragments from intact bovine milk proteins. Mass spectrometry-based peptidomics allows for high throughput sequence assignment of a large number of these peptides. Mastitis is known to result in increased protease activity in the mammary gland. Therefore, we hypothesized that subclinically mastitic milks would contain higher concentrations of released peptides. In this work, milks were sampled from three cows and, for each, one healthy and one subclinically mastitic teat were sampled for milk. Peptides were analyzed by nano-liquid chromatography quadrupole time of flight tandem mass spectrometry and identified with database searching. In total, 682 peptides were identified. The total number of released peptides increased 146% from healthy to subclinically mastitic milks (p <0.05), and the total abundance of released peptides also increased significantly (p <0.05). Bioinformatic analysis of enzyme cleavage revealed increases in activity of cathepsin D and elastase (p <0.05) with subclinical mastitis.

AB - A variety of proteases release hundreds of endogenous peptide fragments from intact bovine milk proteins. Mass spectrometry-based peptidomics allows for high throughput sequence assignment of a large number of these peptides. Mastitis is known to result in increased protease activity in the mammary gland. Therefore, we hypothesized that subclinically mastitic milks would contain higher concentrations of released peptides. In this work, milks were sampled from three cows and, for each, one healthy and one subclinically mastitic teat were sampled for milk. Peptides were analyzed by nano-liquid chromatography quadrupole time of flight tandem mass spectrometry and identified with database searching. In total, 682 peptides were identified. The total number of released peptides increased 146% from healthy to subclinically mastitic milks (p <0.05), and the total abundance of released peptides also increased significantly (p <0.05). Bioinformatic analysis of enzyme cleavage revealed increases in activity of cathepsin D and elastase (p <0.05) with subclinical mastitis.

KW - enzyme inhibitory peptides

KW - globule-membrane proteome

KW - somatic-cell count

KW - casein

KW - proteolysis

KW - hydrolysate

KW - lactation

KW - antibacterial

KW - colostrum

KW - casecidin

U2 - 10.1016/j.idairyj.2014.09.006

DO - 10.1016/j.idairyj.2014.09.006

M3 - Article

VL - 46

SP - 46

EP - 52

JO - International Dairy Journal

T2 - International Dairy Journal

JF - International Dairy Journal

SN - 0958-6946

ER -

Guerrero A, Dallas DC, Contreras S, Bhandari A, Canovas A, Islas-Trejo A et al. Peptidomic analysis of healthy and subclinically mastitic bovine milk. International Dairy Journal. 2015;46:46-52. https://doi.org/10.1016/j.idairyj.2014.09.006