One of the goals of the EU project PEPEIRA (funded under FP6 2007-10, involving 20 partners from 17 European countries) was to carry out a biological characterization of Pepino mosaic virus (PepMV). The objective of the study we report here was twofold: 1) to determine the most important biological characteristics of different PepMV isolates and strains present in the different EU member states, and 2) to determine the possible risks of PepMV strains and variants on tomato and other solanaceous crops.
The development of symptoms and infection rates in local cultivars of important solanaceous cultivated plants and indicator plants were assayed at different European geographic localities for three isolates of PepMV belonging to the European tomato (1066), the Chile2 (PCH06/104) and the US1 genotype. Presence of virus was tested by DAS ELISA and back inoculations to Nicotiana benthamiana and N. occidentalis 37B test plants. In our study, the three selected genotypes of PepMV were inoculated to 16 potato (Solanum tuberosum) cultivars. In general, potato developed no symptoms when inoculated with the three genotypes, and leaves and roots were most often ELISA negative.
|Conference||14th Triennial meeting of the Virology Section of the European Association for Potato Research (EAPR), Hamar, Norway, 4-9 July 2010|
|Period||4/07/10 → 9/07/10|