Peanut allergen Ara h 3: Isolation from peanuts and biochemical characterization.

S.J. Koppelman, E.F. Knol, R.A.A. Vlooswijk, M. Wensing, A.C. Knulst, S.L. Hefle, H. Gruppen, S.R. Piersma

Research output: Contribution to journalArticleAcademicpeer-review

146 Citations (Scopus)

Abstract

Background: Peanut allergen Ara h 3 has been the subject of investigation for the last few years. The reported data strongly depend on recombinant Ara h 3, since a purification protocol for Ara h 3 from peanuts was not available. Methods: Peanut allergen Ara h 3 (glycinin), was purified and its posttranslational processing was investigated. Its allergenic properties were determined by studying IgE binding characteristics of the purified protein. Results: Ara h 3 consists of a series of polypeptides ranging from approximately 14 to 45 kDa that can be classified as acidic and basic subunits, similar to the subunit organization of soy glycinin. N-terminal sequences of the individual polypeptides were determined, and using the cDNA deduced amino-acid sequence, the organization into subunits was explained by revealing posttranslational processing of the different polypeptides. IgE-binding properties of Ara h 3 were investigated using direct elisa and Western blotting with sera from peanut-allergic individuals. The basic subunits, and to a lesser extent the acidic subunits, bind IgE and may act as allergenic peptides. Conclusions: We conclude that peanut-derived Ara h 3, in contrast to earlier reported recombinant Ara h 3, resembles, to a large extent, the molecular organization typical for proteins from the glycinin family. Furthermore, posttranslational processing of Ara h 3 affects the IgE-binding properties and is therefore an essential subject of study for research on the allergenicity of Ara h 3.
Original languageEnglish
Pages (from-to)1144-1151
JournalAllergy
Volume58
Issue number11
DOIs
Publication statusPublished - 2003

Keywords

  • ara-h-i
  • atopic-dermatitis
  • ige binding
  • identification
  • glycinin
  • proteins
  • sera
  • hypersensitivity
  • epitopes
  • cloning

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