PCR detection of oxytetracycline resistance genes from diverse habitats in total community DNA and in streptomycete isolates.

T.L. Nikolakopoulou, S. Egan, L.S. van Overbeek, G. Guillaume, H. Heuer, E.M.H. Wellington, J.D. van Elsas, J.M. Collard, K. Smalla, A.D. Karagouni

    Research output: Contribution to journalArticleAcademicpeer-review

    18 Citations (Scopus)

    Abstract

    A range of European habitats was screened by PCR for detection of the oxytetracycline resistance genes otr(A) and otr(B), found in the oxytetracycline-producing strain Streptomyces rimosus. Primers were developed to detect these otr genes in tetracycline-resistant (TcR) streptomycete isolates from environmental samples. Samples were obtained from bulk and rhizosphere soil, manure, activated sludge and seawater. The majority of TcR streptomycetes originated from bulk and rhizosphere soil. Fewer TcR streptomycetes were isolated from manure and seawater and none from sewage. By PCR, three out of 217 isolates were shown to contain the otr(A) gene and 13 out of 217 the otr(B) gene. Surprisingly, these genes were detected in taxonomic groups not known as tetracycline-producing strains. The majority of the otr gene¿carrying strains was assigned to S. exfoliatus or S. rochei and originated from all habitats from which TcR streptomycetes were obtained. Our results indicated that the occurrence of otr(A) and otr(B) genes in natural environments was limited and that otr(B), in comparison to otr(A), seemed to be more common
    Original languageEnglish
    Pages (from-to)211-216
    JournalCurrent Microbiology
    Volume51
    Issue number4
    DOIs
    Publication statusPublished - 2005

    Fingerprint

    Oxytetracycline
    Ecosystem
    Tetracycline
    Polymerase Chain Reaction
    DNA
    Genes
    Rhizosphere
    Manure
    Seawater
    Sewage
    Streptomyces rimosus
    Soil

    Keywords

    • environmental bacteria
    • efflux protein
    • mycobacterium
    • prevalence
    • validation
    • plasmids
    • clusters
    • rimosus
    • primers
    • soil

    Cite this

    Nikolakopoulou, T.L. ; Egan, S. ; van Overbeek, L.S. ; Guillaume, G. ; Heuer, H. ; Wellington, E.M.H. ; van Elsas, J.D. ; Collard, J.M. ; Smalla, K. ; Karagouni, A.D. / PCR detection of oxytetracycline resistance genes from diverse habitats in total community DNA and in streptomycete isolates. In: Current Microbiology. 2005 ; Vol. 51, No. 4. pp. 211-216.
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    title = "PCR detection of oxytetracycline resistance genes from diverse habitats in total community DNA and in streptomycete isolates.",
    abstract = "A range of European habitats was screened by PCR for detection of the oxytetracycline resistance genes otr(A) and otr(B), found in the oxytetracycline-producing strain Streptomyces rimosus. Primers were developed to detect these otr genes in tetracycline-resistant (TcR) streptomycete isolates from environmental samples. Samples were obtained from bulk and rhizosphere soil, manure, activated sludge and seawater. The majority of TcR streptomycetes originated from bulk and rhizosphere soil. Fewer TcR streptomycetes were isolated from manure and seawater and none from sewage. By PCR, three out of 217 isolates were shown to contain the otr(A) gene and 13 out of 217 the otr(B) gene. Surprisingly, these genes were detected in taxonomic groups not known as tetracycline-producing strains. The majority of the otr gene¿carrying strains was assigned to S. exfoliatus or S. rochei and originated from all habitats from which TcR streptomycetes were obtained. Our results indicated that the occurrence of otr(A) and otr(B) genes in natural environments was limited and that otr(B), in comparison to otr(A), seemed to be more common",
    keywords = "environmental bacteria, efflux protein, mycobacterium, prevalence, validation, plasmids, clusters, rimosus, primers, soil",
    author = "T.L. Nikolakopoulou and S. Egan and {van Overbeek}, L.S. and G. Guillaume and H. Heuer and E.M.H. Wellington and {van Elsas}, J.D. and J.M. Collard and K. Smalla and A.D. Karagouni",
    year = "2005",
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    language = "English",
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    Nikolakopoulou, TL, Egan, S, van Overbeek, LS, Guillaume, G, Heuer, H, Wellington, EMH, van Elsas, JD, Collard, JM, Smalla, K & Karagouni, AD 2005, 'PCR detection of oxytetracycline resistance genes from diverse habitats in total community DNA and in streptomycete isolates.', Current Microbiology, vol. 51, no. 4, pp. 211-216. https://doi.org/10.1007/s00284-004-4430-4

    PCR detection of oxytetracycline resistance genes from diverse habitats in total community DNA and in streptomycete isolates. / Nikolakopoulou, T.L.; Egan, S.; van Overbeek, L.S.; Guillaume, G.; Heuer, H.; Wellington, E.M.H.; van Elsas, J.D.; Collard, J.M.; Smalla, K.; Karagouni, A.D.

    In: Current Microbiology, Vol. 51, No. 4, 2005, p. 211-216.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - PCR detection of oxytetracycline resistance genes from diverse habitats in total community DNA and in streptomycete isolates.

    AU - Nikolakopoulou, T.L.

    AU - Egan, S.

    AU - van Overbeek, L.S.

    AU - Guillaume, G.

    AU - Heuer, H.

    AU - Wellington, E.M.H.

    AU - van Elsas, J.D.

    AU - Collard, J.M.

    AU - Smalla, K.

    AU - Karagouni, A.D.

    PY - 2005

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    N2 - A range of European habitats was screened by PCR for detection of the oxytetracycline resistance genes otr(A) and otr(B), found in the oxytetracycline-producing strain Streptomyces rimosus. Primers were developed to detect these otr genes in tetracycline-resistant (TcR) streptomycete isolates from environmental samples. Samples were obtained from bulk and rhizosphere soil, manure, activated sludge and seawater. The majority of TcR streptomycetes originated from bulk and rhizosphere soil. Fewer TcR streptomycetes were isolated from manure and seawater and none from sewage. By PCR, three out of 217 isolates were shown to contain the otr(A) gene and 13 out of 217 the otr(B) gene. Surprisingly, these genes were detected in taxonomic groups not known as tetracycline-producing strains. The majority of the otr gene¿carrying strains was assigned to S. exfoliatus or S. rochei and originated from all habitats from which TcR streptomycetes were obtained. Our results indicated that the occurrence of otr(A) and otr(B) genes in natural environments was limited and that otr(B), in comparison to otr(A), seemed to be more common

    AB - A range of European habitats was screened by PCR for detection of the oxytetracycline resistance genes otr(A) and otr(B), found in the oxytetracycline-producing strain Streptomyces rimosus. Primers were developed to detect these otr genes in tetracycline-resistant (TcR) streptomycete isolates from environmental samples. Samples were obtained from bulk and rhizosphere soil, manure, activated sludge and seawater. The majority of TcR streptomycetes originated from bulk and rhizosphere soil. Fewer TcR streptomycetes were isolated from manure and seawater and none from sewage. By PCR, three out of 217 isolates were shown to contain the otr(A) gene and 13 out of 217 the otr(B) gene. Surprisingly, these genes were detected in taxonomic groups not known as tetracycline-producing strains. The majority of the otr gene¿carrying strains was assigned to S. exfoliatus or S. rochei and originated from all habitats from which TcR streptomycetes were obtained. Our results indicated that the occurrence of otr(A) and otr(B) genes in natural environments was limited and that otr(B), in comparison to otr(A), seemed to be more common

    KW - environmental bacteria

    KW - efflux protein

    KW - mycobacterium

    KW - prevalence

    KW - validation

    KW - plasmids

    KW - clusters

    KW - rimosus

    KW - primers

    KW - soil

    U2 - 10.1007/s00284-004-4430-4

    DO - 10.1007/s00284-004-4430-4

    M3 - Article

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    SP - 211

    EP - 216

    JO - Current Microbiology

    JF - Current Microbiology

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