PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures

R. Valente; F.C. Gouveia; G.A. de Lemos; D. Pimentel; J.D. van Elsas; L.C. Mendonca-Hagler; A.N. Hagler

doi:10.1111/j.1574-6968.1996.tb08114.x

PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures

R. Valente, F.C. Gouveia, G.A. de Lemos, D. Pimentel, J.D. van Elsas, L.C. Mendonca-Hagler, A.N. Hagler

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Abstract

The size of the internal transcribed spacer (ITS) region as measured by gel electrophoresis of PCR products, amplified by primers ITS1 and ITS4, was over 800 bp for all Saccharomyces sensu stricto species, but yeasts belonging to other Saccharomyces species had a shorter ITS region, making this characteristic potentially useful in the identification of Saccharomyces isolates. The ITS product length was homogeneous within the species Saccharomyces cerevisiae.

Original language	English
Pages (from-to)	253-256
Journal	FEMS Microbiology Letters
Volume	137
DOIs	https://doi.org/10.1111/j.1574-6968.1996.tb08114.x
Publication status	Published - 1996

Keywords

internal transcribed spacer
PCR
Saccharomyces
yeast taxonomy

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10.1111/j.1574-6968.1996.tb08114.x

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title = "PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures",

abstract = "The size of the internal transcribed spacer (ITS) region as measured by gel electrophoresis of PCR products, amplified by primers ITS1 and ITS4, was over 800 bp for all Saccharomyces sensu stricto species, but yeasts belonging to other Saccharomyces species had a shorter ITS region, making this characteristic potentially useful in the identification of Saccharomyces isolates. The ITS product length was homogeneous within the species Saccharomyces cerevisiae.",

keywords = "internal transcribed spacer, PCR, Saccharomyces, yeast taxonomy",

author = "R. Valente and F.C. Gouveia and {de Lemos}, G.A. and D. Pimentel and {van Elsas}, J.D. and L.C. Mendonca-Hagler and A.N. Hagler",

year = "1996",

doi = "10.1111/j.1574-6968.1996.tb08114.x",

language = "English",

volume = "137",

pages = "253--256",

journal = "FEMS Microbiology Letters",

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T1 - PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures

AU - Valente, R.

AU - Gouveia, F.C.

AU - de Lemos, G.A.

AU - Pimentel, D.

AU - van Elsas, J.D.

AU - Mendonca-Hagler, L.C.

AU - Hagler, A.N.

PY - 1996

Y1 - 1996

N2 - The size of the internal transcribed spacer (ITS) region as measured by gel electrophoresis of PCR products, amplified by primers ITS1 and ITS4, was over 800 bp for all Saccharomyces sensu stricto species, but yeasts belonging to other Saccharomyces species had a shorter ITS region, making this characteristic potentially useful in the identification of Saccharomyces isolates. The ITS product length was homogeneous within the species Saccharomyces cerevisiae.

AB - The size of the internal transcribed spacer (ITS) region as measured by gel electrophoresis of PCR products, amplified by primers ITS1 and ITS4, was over 800 bp for all Saccharomyces sensu stricto species, but yeasts belonging to other Saccharomyces species had a shorter ITS region, making this characteristic potentially useful in the identification of Saccharomyces isolates. The ITS product length was homogeneous within the species Saccharomyces cerevisiae.

KW - internal transcribed spacer

KW - PCR

KW - Saccharomyces

KW - yeast taxonomy

U2 - 10.1111/j.1574-6968.1996.tb08114.x

DO - 10.1111/j.1574-6968.1996.tb08114.x

M3 - Article

SN - 0378-1097

VL - 137

SP - 253

EP - 256

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

ER -

PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures

Abstract

Keywords

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