PCIB an antiauxin enhances microspore embryogenisis in microspore culture of Brassica juncea

P.K. Agarwal, P. Agarwal, J.B.M. Custers, C.M. Liu, S.S. Bhojwani

    Research output: Contribution to journalArticleAcademicpeer-review

    31 Citations (Scopus)

    Abstract

    An efficient protocol to improve microspore embryogenesis is established in an important oleiferous crop, Brassica juncea (Indian mustard). Colchicine was used for enhancing microspore embryogenesis and also to obtain doubled haploid embryos. Colchicine at high concentrations (>10 mg l¿1), for 24 h, proved convenient for direct recovery of diploid embryos. Higher temperature treatment and an antiauxin PCIB (p-chlorophenoxyisobutyric acid) enhanced microspore embryogenesis significantly as compared to colchicine. An increase in temperature from 32°C to 35°C proved very efficient in increasing embryogenesis by 10-fold. The highest embryogenesis rate was obtained when PCIB was added at 35°C in the culture after 1 day of culture initiation. 20 ¿M PCIB could enhance microspore embryogenesis by 5-fold. Different abnormal shapes of embryos like lemon, banana, flask and fused cotyledons were observed. Both normal and fused cotyledonous embryos showed normal germination when transferred on the B5 basal medium
    Original languageEnglish
    Pages (from-to)201-210
    JournalPlant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
    Volume86
    Issue number2
    DOIs
    Publication statusPublished - 2006

    Keywords

    • doubled haploid plants
    • immediate colchicine treatment
    • napus cv topas
    • oilseed rape
    • desiccation tolerance
    • efficient production
    • anther cultures
    • heat-shock
    • embryos
    • induction

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