Patterning of Peptide Nucleic Acids Using Reactive Microcontact Printing

A. Calabretta, D. Wasserberg, G.A. Posthuma-Trumpie, V. Subramaniam, A. van Amerongen, R. Corradini, T. Tedeschi, S. Sforza, D.N. Reinhoudt, R. Marchelli, J. Huskens, P. Jonkheijm

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)

Abstract

PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive microcontact printing using oxygen-oxidized polydimethylsiloxane stamps, homogeneous arrays were fabricated and characterized using optical methods. PNA-patterned surfaces were hybridized with complementary and mismatched dye-labeled oligonucleotides to test their ability to recognize DNA sequences. The stability and selectivity of the PNA-DNA duplexes on surfaces have been verified by fluorescence microscopy, and the melting curves have been recorded. Finally, the technique has been applied to the fabrication of chips by spotting a PNA microarray onto a flat PDMS stamp and reproducing the same features onto many slides. The chips were finally applied to single nucleotide polymorphism detection on oligonucleotides.
Original languageEnglish
Pages (from-to)1536-1542
JournalLangmuir
Volume27
Issue number4
DOIs
Publication statusPublished - 2011

Keywords

  • stereogenic centers
  • dna biosensors
  • pna
  • microarrays
  • nanoparticle
  • recognition
  • immobilization
  • hybridization
  • handedness
  • proteins

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