Abstract
Microorganisms living inside plants can promote plant growth and health, but their genomic and functional diversity remain largely elusive. Here, metagenomics and network inference show that fungal infection of plant roots enriched for Chitinophagaceae and Flavobacteriaceae in the root endosphere and for chitinase genes and various unknown biosynthetic gene clusters encoding the production of nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). After strain-level genome reconstruction, a consortium of Chitinophaga and Flavobacterium was designed that consistently suppressed fungal root disease. Site-directed mutagenesis then revealed that a previously unidentified NRPS-PKS gene cluster from Flavobacterium was essential for disease suppression by the endophytic consortium. Our results highlight that endophytic root microbiomes harbor a wealth of as yet unknown functional traits that, in concert, can protect the plant inside out.
| Original language | English |
|---|---|
| Pages (from-to) | 606-612 |
| Number of pages | 7 |
| Journal | Science |
| Volume | 366 |
| Issue number | 6465 |
| DOIs | |
| Publication status | Published - 1 Nov 2019 |
Fingerprint
Cite this
}
Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome. / Carrión, Víctor J.; Perez-Jaramillo, Juan; Cordovez, Viviane; Tracanna, Vittorio; De Hollander, Mattias; Ruiz-Buck, Daniel; Mendes, Lucas W.; van Ijcken, Wilfred F.J.; Gomez-Exposito, Ruth; Elsayed, Somayah S.; Mohanraju, Prarthana; Arifah, Adini; van der Oost, John; Paulson, Joseph N.; Mendes, Rodrigo; van Wezel, Gilles P.; Medema, Marnix H.; Raaijmakers, Jos M.
In: Science, Vol. 366, No. 6465, 01.11.2019, p. 606-612.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome
AU - Carrión, Víctor J.
AU - Perez-Jaramillo, Juan
AU - Cordovez, Viviane
AU - Tracanna, Vittorio
AU - De Hollander, Mattias
AU - Ruiz-Buck, Daniel
AU - Mendes, Lucas W.
AU - van Ijcken, Wilfred F.J.
AU - Gomez-Exposito, Ruth
AU - Elsayed, Somayah S.
AU - Mohanraju, Prarthana
AU - Arifah, Adini
AU - van der Oost, John
AU - Paulson, Joseph N.
AU - Mendes, Rodrigo
AU - van Wezel, Gilles P.
AU - Medema, Marnix H.
AU - Raaijmakers, Jos M.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - Microorganisms living inside plants can promote plant growth and health, but their genomic and functional diversity remain largely elusive. Here, metagenomics and network inference show that fungal infection of plant roots enriched for Chitinophagaceae and Flavobacteriaceae in the root endosphere and for chitinase genes and various unknown biosynthetic gene clusters encoding the production of nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). After strain-level genome reconstruction, a consortium of Chitinophaga and Flavobacterium was designed that consistently suppressed fungal root disease. Site-directed mutagenesis then revealed that a previously unidentified NRPS-PKS gene cluster from Flavobacterium was essential for disease suppression by the endophytic consortium. Our results highlight that endophytic root microbiomes harbor a wealth of as yet unknown functional traits that, in concert, can protect the plant inside out.
AB - Microorganisms living inside plants can promote plant growth and health, but their genomic and functional diversity remain largely elusive. Here, metagenomics and network inference show that fungal infection of plant roots enriched for Chitinophagaceae and Flavobacteriaceae in the root endosphere and for chitinase genes and various unknown biosynthetic gene clusters encoding the production of nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). After strain-level genome reconstruction, a consortium of Chitinophaga and Flavobacterium was designed that consistently suppressed fungal root disease. Site-directed mutagenesis then revealed that a previously unidentified NRPS-PKS gene cluster from Flavobacterium was essential for disease suppression by the endophytic consortium. Our results highlight that endophytic root microbiomes harbor a wealth of as yet unknown functional traits that, in concert, can protect the plant inside out.
U2 - 10.1126/science.aaw9285
DO - 10.1126/science.aaw9285
M3 - Article
VL - 366
SP - 606
EP - 612
JO - Science
JF - Science
SN - 0036-8075
IS - 6465
ER -