Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge

Jasmine Tomar, Carin Biel, Cornelis A.M. de Haan, Peter J.M. Rottier, Nikolai Petrovsky, Henderik W. Frijlink, Anke Huckriede, Wouter L.J. Hinrichs, Ben Peeters

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Bird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, suitable formulations and an effective mass vaccination method that can be translated to field settings needs to be developed. Our previous study in chickens has shown that inhalation of a non-adjuvanted dry powder influenza vaccine formulation during normal breathing results in partial protection against lethal influenza challenge. The aim of the present study was to improve the effectiveness of pulmonary vaccination by increasing the vaccine dose deposited in the lungs and by the use of suitable adjuvants. Two adjuvants, namely, Bacterium-like Particles (BLP) and Advax, were spray freeze dried with influenza vaccine into dry powder formulations. Delivery of dry formulations directly at the syrinx revealed that BLP and Advax had the potential to boost either systemic or mucosal immune responses or both. Upon passive inhalation of dry influenza vaccine formulations in an optimized set-up, BLP and Advax/BLP adjuvanted formulations induced significantly higher systemic immune responses than the non-adjuvanted formulation. Remarkably, all vaccinated animals not only survived a lethal influenza challenge, but also did not show any shedding of challenge virus except for two out of six animals in the Advax group. Overall, our results indicate that passive inhalation is feasible, effective and suitable for mass vaccination of chickens if it can be adapted to field settings.

Original languageEnglish
Pages (from-to)85-95
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume133
DOIs
Publication statusPublished - Dec 2018

Fingerprint

Influenza Vaccines
Powders
Inhalation
Chickens
Mass Vaccination
Bacteria
Influenza in Birds
Orthomyxoviridae
Human Influenza
Virulence
Vaccination
Virus Shedding
Mucosal Immunity
Lung
Syringes
Pandemics
Poultry
Birds
Disease Outbreaks
Respiration

Keywords

  • Adjuvants
  • Challenge
  • Influenza
  • Inhalation
  • Passive
  • Powders
  • Protection
  • Pulmonary

Cite this

Tomar, Jasmine ; Biel, Carin ; de Haan, Cornelis A.M. ; Rottier, Peter J.M. ; Petrovsky, Nikolai ; Frijlink, Henderik W. ; Huckriede, Anke ; Hinrichs, Wouter L.J. ; Peeters, Ben. / Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge. In: European Journal of Pharmaceutics and Biopharmaceutics. 2018 ; Vol. 133. pp. 85-95.
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abstract = "Bird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, suitable formulations and an effective mass vaccination method that can be translated to field settings needs to be developed. Our previous study in chickens has shown that inhalation of a non-adjuvanted dry powder influenza vaccine formulation during normal breathing results in partial protection against lethal influenza challenge. The aim of the present study was to improve the effectiveness of pulmonary vaccination by increasing the vaccine dose deposited in the lungs and by the use of suitable adjuvants. Two adjuvants, namely, Bacterium-like Particles (BLP) and Advax, were spray freeze dried with influenza vaccine into dry powder formulations. Delivery of dry formulations directly at the syrinx revealed that BLP and Advax had the potential to boost either systemic or mucosal immune responses or both. Upon passive inhalation of dry influenza vaccine formulations in an optimized set-up, BLP and Advax/BLP adjuvanted formulations induced significantly higher systemic immune responses than the non-adjuvanted formulation. Remarkably, all vaccinated animals not only survived a lethal influenza challenge, but also did not show any shedding of challenge virus except for two out of six animals in the Advax group. Overall, our results indicate that passive inhalation is feasible, effective and suitable for mass vaccination of chickens if it can be adapted to field settings.",
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Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge. / Tomar, Jasmine; Biel, Carin; de Haan, Cornelis A.M.; Rottier, Peter J.M.; Petrovsky, Nikolai; Frijlink, Henderik W.; Huckriede, Anke; Hinrichs, Wouter L.J.; Peeters, Ben.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 133, 12.2018, p. 85-95.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge

AU - Tomar, Jasmine

AU - Biel, Carin

AU - de Haan, Cornelis A.M.

AU - Rottier, Peter J.M.

AU - Petrovsky, Nikolai

AU - Frijlink, Henderik W.

AU - Huckriede, Anke

AU - Hinrichs, Wouter L.J.

AU - Peeters, Ben

PY - 2018/12

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N2 - Bird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, suitable formulations and an effective mass vaccination method that can be translated to field settings needs to be developed. Our previous study in chickens has shown that inhalation of a non-adjuvanted dry powder influenza vaccine formulation during normal breathing results in partial protection against lethal influenza challenge. The aim of the present study was to improve the effectiveness of pulmonary vaccination by increasing the vaccine dose deposited in the lungs and by the use of suitable adjuvants. Two adjuvants, namely, Bacterium-like Particles (BLP) and Advax, were spray freeze dried with influenza vaccine into dry powder formulations. Delivery of dry formulations directly at the syrinx revealed that BLP and Advax had the potential to boost either systemic or mucosal immune responses or both. Upon passive inhalation of dry influenza vaccine formulations in an optimized set-up, BLP and Advax/BLP adjuvanted formulations induced significantly higher systemic immune responses than the non-adjuvanted formulation. Remarkably, all vaccinated animals not only survived a lethal influenza challenge, but also did not show any shedding of challenge virus except for two out of six animals in the Advax group. Overall, our results indicate that passive inhalation is feasible, effective and suitable for mass vaccination of chickens if it can be adapted to field settings.

AB - Bird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, suitable formulations and an effective mass vaccination method that can be translated to field settings needs to be developed. Our previous study in chickens has shown that inhalation of a non-adjuvanted dry powder influenza vaccine formulation during normal breathing results in partial protection against lethal influenza challenge. The aim of the present study was to improve the effectiveness of pulmonary vaccination by increasing the vaccine dose deposited in the lungs and by the use of suitable adjuvants. Two adjuvants, namely, Bacterium-like Particles (BLP) and Advax, were spray freeze dried with influenza vaccine into dry powder formulations. Delivery of dry formulations directly at the syrinx revealed that BLP and Advax had the potential to boost either systemic or mucosal immune responses or both. Upon passive inhalation of dry influenza vaccine formulations in an optimized set-up, BLP and Advax/BLP adjuvanted formulations induced significantly higher systemic immune responses than the non-adjuvanted formulation. Remarkably, all vaccinated animals not only survived a lethal influenza challenge, but also did not show any shedding of challenge virus except for two out of six animals in the Advax group. Overall, our results indicate that passive inhalation is feasible, effective and suitable for mass vaccination of chickens if it can be adapted to field settings.

KW - Adjuvants

KW - Challenge

KW - Influenza

KW - Inhalation

KW - Passive

KW - Powders

KW - Protection

KW - Pulmonary

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M3 - Article

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EP - 95

JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

SN - 0939-6411

ER -