Partial replacement of glucose by galactose in the post-weaning diet improves parameters of hepatic health

Lianne M.S. Bouwman, Hans J.M. Swarts, José M.S. Fernández-Calleja, Inge van der Stelt, Henk Schols, Annemarie Oosting, Jaap Keijer, Evert M. van Schothorst*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice. The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known. The aim of this study was to elucidate this. Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL). Body weight, body composition, and food intake were determined weekly. After 3 weeks, mice were sacrificed, and serum and liver tissues were collected. Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays. Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet. Hepatic TG content was lower in GLU+GAL-fed than GLU-fed females, while glycogen levels were unaffected. Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females. This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3. In conclusion, replacing part of glucose with galactose in the post-weaning diet reduces hepatic TG content and hepatic inflammation.

Original languageEnglish
Article number108223
JournalJournal of Nutritional Biochemistry
Volume73
DOIs
Publication statusPublished - Nov 2019

Fingerprint

Nutrition
Weaning
Galactose
Health
Diet
Glucose
Liver
Triglycerides
Glycogen
Body Composition
Inflammation
Serum Amyloid A Protein
Messenger RNA
Acute-Phase Proteins
Monosaccharides
Eating
Body Weight
Chemical analysis
Metabolism
Starch

Keywords

  • Galactose
  • Inflammation
  • Liver health
  • Post-weaning diet
  • SAA3
  • Transcriptomics

Cite this

@article{ce27597829de41c296e5a7743d9ededa,
title = "Partial replacement of glucose by galactose in the post-weaning diet improves parameters of hepatic health",
abstract = "Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice. The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known. The aim of this study was to elucidate this. Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en{\%}) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en{\%}) (GLU+GAL). Body weight, body composition, and food intake were determined weekly. After 3 weeks, mice were sacrificed, and serum and liver tissues were collected. Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays. Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet. Hepatic TG content was lower in GLU+GAL-fed than GLU-fed females, while glycogen levels were unaffected. Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females. This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3. In conclusion, replacing part of glucose with galactose in the post-weaning diet reduces hepatic TG content and hepatic inflammation.",
keywords = "Galactose, Inflammation, Liver health, Post-weaning diet, SAA3, Transcriptomics",
author = "Bouwman, {Lianne M.S.} and Swarts, {Hans J.M.} and Fern{\'a}ndez-Calleja, {Jos{\'e} M.S.} and {van der Stelt}, Inge and Henk Schols and Annemarie Oosting and Jaap Keijer and {van Schothorst}, {Evert M.}",
year = "2019",
month = "11",
doi = "10.1016/j.jnutbio.2019.108223",
language = "English",
volume = "73",
journal = "Journal of Nutritional Biochemistry",
issn = "0955-2863",
publisher = "Elsevier",

}

Partial replacement of glucose by galactose in the post-weaning diet improves parameters of hepatic health. / Bouwman, Lianne M.S.; Swarts, Hans J.M.; Fernández-Calleja, José M.S.; van der Stelt, Inge; Schols, Henk; Oosting, Annemarie; Keijer, Jaap; van Schothorst, Evert M.

In: Journal of Nutritional Biochemistry, Vol. 73, 108223, 11.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Partial replacement of glucose by galactose in the post-weaning diet improves parameters of hepatic health

AU - Bouwman, Lianne M.S.

AU - Swarts, Hans J.M.

AU - Fernández-Calleja, José M.S.

AU - van der Stelt, Inge

AU - Schols, Henk

AU - Oosting, Annemarie

AU - Keijer, Jaap

AU - van Schothorst, Evert M.

PY - 2019/11

Y1 - 2019/11

N2 - Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice. The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known. The aim of this study was to elucidate this. Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL). Body weight, body composition, and food intake were determined weekly. After 3 weeks, mice were sacrificed, and serum and liver tissues were collected. Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays. Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet. Hepatic TG content was lower in GLU+GAL-fed than GLU-fed females, while glycogen levels were unaffected. Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females. This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3. In conclusion, replacing part of glucose with galactose in the post-weaning diet reduces hepatic TG content and hepatic inflammation.

AB - Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice. The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known. The aim of this study was to elucidate this. Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL). Body weight, body composition, and food intake were determined weekly. After 3 weeks, mice were sacrificed, and serum and liver tissues were collected. Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays. Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet. Hepatic TG content was lower in GLU+GAL-fed than GLU-fed females, while glycogen levels were unaffected. Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females. This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3. In conclusion, replacing part of glucose with galactose in the post-weaning diet reduces hepatic TG content and hepatic inflammation.

KW - Galactose

KW - Inflammation

KW - Liver health

KW - Post-weaning diet

KW - SAA3

KW - Transcriptomics

U2 - 10.1016/j.jnutbio.2019.108223

DO - 10.1016/j.jnutbio.2019.108223

M3 - Article

VL - 73

JO - Journal of Nutritional Biochemistry

JF - Journal of Nutritional Biochemistry

SN - 0955-2863

M1 - 108223

ER -