Partial Functional Rescue of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus Infectivity by Replacement of F Protein with GP64 from Autographa californica Multicapsid Nucleopolyhedrovirus

M.L. Wang, F.F. Yin, S. Shen, Y. Tan, F. Deng, J.M. Vlak, Z.H. Hu, H.L. Wang

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Two distinct envelope fusion proteins (EFPs) (GP64 and F) have been identified in members of the Baculoviridae family of viruses. F proteins are found in group II nucleopolyhedroviruses (NPVs) of alphabaculoviruses and in beta- and deltabaculoviruses, while GP64 occurs only in group I NPVs of alphabaculoviruses. It was proposed that an ancestral baculovirus acquired the gp64 gene that conferred a selective advantage and allowed it to evolve into group I NPVs. The F protein is a functional analogue of GP64, as evidenced from the rescue of gp64-null Autographa californica multicapsid nucleopolyhedrovirus (MNPV) (AcMNPV) by F proteins from group II NPVs or from betabaculoviruses. However, GP64 failed to rescue an F-null Spodoptera exigua MNPV (SeMNPV) (group II NPV). Here, we report the successful generation of an infectious gp64-rescued group II NPV of Helicoverpa armigera (vHaBac Delta F-gp64). Viral growth curve assays and quantitative real-time PCR (Q-PCR), however, showed substantially decreased infectivity of vHaBac Delta F-gp64 compared to the HaF rescue control virus vHaBac Delta F-HaF. Electron microscopy further showed that most vHaBac Delta F-gp64 budded viruses (BV) in the cell culture supernatant lacked envelope components and contained morphologically aberrant nucleocapsids, suggesting the improper BV envelopment or budding of vHaBac Delta F-gp64. Bioassays using pseudotyped viruses with a reintroduced polyhedrin gene showed that GP64-pseudotyped Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) significantly delayed the mortality of infected H. armigera larvae.
Original languageEnglish
Pages (from-to)11505-11514
JournalJournal of Virology
Volume84
Issue number21
DOIs
Publication statusPublished - 2010

Keywords

  • envelope fusion protein
  • nuclear polyhedrosis-virus
  • baculovirus gp64
  • lentiviral vectors
  • membrane-fusion
  • glycoproteins
  • cells
  • homolog
  • entry
  • ac23

Fingerprint Dive into the research topics of 'Partial Functional Rescue of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus Infectivity by Replacement of F Protein with GP64 from Autographa californica Multicapsid Nucleopolyhedrovirus'. Together they form a unique fingerprint.

Cite this