TY - JOUR
T1 - Partial acid-hydrolysis of TEMPO-oxidized arabinoxylans generates arabinoxylan-structure resembling oligosaccharides
AU - Pandeirada, Carolina O.
AU - Speranza, Sofia
AU - Bakx, Edwin
AU - Westphal, Yvonne
AU - Janssen, Hans Gerd
AU - Schols, Henk A.
N1 - Publisher Copyright:
© 2021 The Author(s)
PY - 2022/1/15
Y1 - 2022/1/15
N2 - Arabinoxylans (AXs) display biological activities that depend on their chemical structures. To structurally characterize and distinguish AXs using a non-enzymatic approach, various TEMPO-oxidized AXs were partially acid-hydrolysed to obtain diagnostic oligosaccharides (OS). Arabinurono-xylo-oligomer alditols (AUXOS-A) with degree of polymerization 2–5, comprising one and two arabinuronic acid (AraA) substituents were identified in the UHPLC-PGC–MS profiles of three TEMPO-oxidized AXs, namely wheat (ox-WAX), partially-debranched WAX (ox-pD-WAX), and rye (ox-RAX). Characterization of these AUXOS-A highlighted that single-substitution of the Xyl unit preferably occurs at position O-3 for these samples, and that ox-WAX has both more single substituted and more double-substituted xylose residues in its backbone than the other AXs. Characteristic UHPLC-PGC–MS OS profiles, differing in OS abundance and composition, were obtained for each AX. Thus, partial acid-hydrolysis of TEMPO-oxidized AXs with analysis of the released OS by UHPLC-PGC-MS is a promising novel non-enzymatic approach to distinguish AXs and obtain insights into their structures.
AB - Arabinoxylans (AXs) display biological activities that depend on their chemical structures. To structurally characterize and distinguish AXs using a non-enzymatic approach, various TEMPO-oxidized AXs were partially acid-hydrolysed to obtain diagnostic oligosaccharides (OS). Arabinurono-xylo-oligomer alditols (AUXOS-A) with degree of polymerization 2–5, comprising one and two arabinuronic acid (AraA) substituents were identified in the UHPLC-PGC–MS profiles of three TEMPO-oxidized AXs, namely wheat (ox-WAX), partially-debranched WAX (ox-pD-WAX), and rye (ox-RAX). Characterization of these AUXOS-A highlighted that single-substitution of the Xyl unit preferably occurs at position O-3 for these samples, and that ox-WAX has both more single substituted and more double-substituted xylose residues in its backbone than the other AXs. Characteristic UHPLC-PGC–MS OS profiles, differing in OS abundance and composition, were obtained for each AX. Thus, partial acid-hydrolysis of TEMPO-oxidized AXs with analysis of the released OS by UHPLC-PGC-MS is a promising novel non-enzymatic approach to distinguish AXs and obtain insights into their structures.
KW - Arabinoxylan
KW - Partial acid-hydrolysis
KW - TEMPO-oxidation
KW - UHPLC-PGC-MS
U2 - 10.1016/j.carbpol.2021.118795
DO - 10.1016/j.carbpol.2021.118795
M3 - Article
AN - SCOPUS:85117731235
SN - 0144-8617
VL - 276
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 118795
ER -