Parageobacillus thermoglucosidasius as an emerging thermophilic cell factory

Miguel Paredes-Barrada, Panagiotis Kopsiaftis, Nico J. Claassens*, Richard van Kranenburg*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Parageobacillus thermoglucosidasius is a thermophilic and facultatively anaerobic microbe, which is emerging as one of the most promising thermophilic model organisms for metabolic engineering. The use of thermophilic microorganisms for industrial bioprocesses provides the advantages of increased reaction rates and reduced cooling costs for bioreactors compared to their mesophilic counterparts. Moreover, it enables starch or lignocellulose degradation and fermentation to occur at the same temperature in a Simultaneous Saccharification and Fermentation (SSF) or Consolidated Bioprocessing (CBP) approach. Its natural hemicellulolytic capabilities and its ability to convert CO to metabolic energy make P. thermoglucosidasius a potentially attractive host for bio-based processes. It can effectively degrade hemicellulose due to a number of hydrolytic enzymes, carbohydrate transporters, and regulatory elements coded from a genomic cluster named Hemicellulose Utilization (HUS) locus. The growing availability of effective genetic engineering tools in P. thermoglucosidasius further starts to open up its potential as a versatile thermophilic cell factory. A number of strain engineering examples showcasing the potential of P. thermoglucosidasius as a microbial chassis for the production of bulk and fine chemicals are presented along with current research bottlenecks. Ultimately, this review provides a holistic overview of the distinct metabolic characteristics of P. thermoglucosidasius and discusses research focused on expanding the native metabolic boundaries for the development of industrially relevant strains.

Original languageEnglish
Pages (from-to)39-51
Number of pages13
JournalMetabolic Engineering
Publication statusPublished - May 2024


  • Alternative feedstocks
  • Hemicellulose utilization
  • Metabolic engineering
  • Microbial cell factories
  • Parageobacillus thermoglucosidasius
  • Thermophilic organism


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