Palmitic acid follows a different metabolic pathway than oleic acid in human skeletal muscle cells; lower lipolysis rate despite an increased level of adipose triglyceride lipase

S.S. Bakke, C. Moro, N. Nikolic, N.P. Hessvik, P.M. Badin, L. Lauvhaug, K. Fredriksson, M.K. Hesselink, M.V. Boekschoten, A.H. Kersten, G.H. Thoresen, A.C. Rustan

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21 Citations (Scopus)

Abstract

Development of insulin resistance is positively associated with dietary saturated fatty acids and negatively associated with monounsaturated fatty acids. To clarify aspects of this difference we have compared the metabolism of oleic (OA, monounsaturated) and palmitic acids (PA, saturated) in human myotubes. Human myotubes were treated with 100 µM OA or PA and the metabolism of [14C]-labeled fatty acid was studied. We observed that PA had a lower lipolysis rate than OA, despite a more than two-fold higher protein level of adipose triglyceride lipase after 24 h incubation with PA. PA was less incorporated into triacylglycerol and more incorporated into phospholipids after 24 h. Supporting this, incubation with compounds modifying lipolysis and reesterification pathways suggested a less influenced PA than OA metabolism. In addition, PA showed a lower accumulation than OA, though PA was oxidized to a relatively higher extent than OA. Gene set enrichment analysis revealed that 24 h of PA treatment upregulated lipogenesis and fatty acid ß-oxidation and downregulated oxidative phosphorylation compared to OA. The differences in lipid accumulation and lipolysis between OA and PA were eliminated in combination with eicosapentaenoic acid (polyunsaturated fatty acid). In conclusion, this study reveals that the two most abundant fatty acids in our diet are partitioned toward different metabolic pathways in muscle cells, and this may be relevant to understand the link between dietary fat and skeletal muscle insulin resistance.
Original languageEnglish
Pages (from-to)1323-1333
Number of pages11
JournalBiochimica et Biophysica Acta. Molecular and Cell Biology of Lipids
Volume1821
Issue number10
DOIs
Publication statusPublished - 2012

Keywords

  • hormone-sensitive lipase
  • type-2 diabetic subjects
  • chanarin-dorfman-syndrome
  • reduced lipid oxidation
  • saturated fatty-acids
  • chain acyl-coa
  • insulin-resistance
  • human myotubes
  • mediated lipolysis
  • cultured myotubes

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  • Datasets

    Eicosapentaenoic acid improves metabolic switching in human myotubes

    Hessvik, N. P. (Creator), Bakke, S. S. (Creator), Fredriksson, K. (Creator), Boekschoten, M. (Creator), Fjorkenstad, A. (Creator), Koster, G. (Creator), Hesselink, M. K. (Creator), Kersten, S. (Creator), Kase, E. T. (Creator), Rustan, A. C. (Creator) & Thoresen, H. (Creator), Wageningen University, 23 Jun 2010

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