P-fimbriae of Escherichia coli as carriers for gonadotropin releasing hormone: development of a recombinant contraceptive vaccine

A. van der Zee, C.V. Noordegraaf, H. van den Bosch, J. Gielen, H. Bergmans, W. Hoekstra, I. van Die

    Research output: Contribution to journalArticleAcademicpeer-review

    17 Citations (Scopus)

    Abstract

    The demand for an effective and low cost means of fertility control of domestic animals has raised interest in the development of contraceptive vaccines. A promising candidate for a vaccine component is the brain peptide gonadotropin releasing hormone (GnRH), which plays a central role in the regulation of reproductive functions in vertebrates. Neutralization of GnRH by vaccine-induced antibodies is expected to prevent the reproductive activity in a wide range of species. A GnRH-protein conjugate was prepared by means of recombinant DNA technology. The oligonucleotides encoding GnRH were inserted in hypervariable regions of the subunit gene of P-fimbriae of Escherichia coli. Hybrid fimbriae encoded by the manipulated fimbrial gene clusters were expressed efficiently on the cell surface of Escherichia coli. Vaccination of female rats and bull calves with purified hybrid GnRH-fimbriae strongly affected the reproductive characteristics. We conclude that P-fimbriae represent a very attractive carrier system for application of GnRH in a new type of vaccine.
    Original languageEnglish
    Pages (from-to)753-758
    JournalVaccine
    Volume13
    Issue number8
    DOIs
    Publication statusPublished - 1995

    Fingerprint Dive into the research topics of 'P-fimbriae of Escherichia coli as carriers for gonadotropin releasing hormone: development of a recombinant contraceptive vaccine'. Together they form a unique fingerprint.

  • Cite this

    van der Zee, A., Noordegraaf, C. V., van den Bosch, H., Gielen, J., Bergmans, H., Hoekstra, W., & van Die, I. (1995). P-fimbriae of Escherichia coli as carriers for gonadotropin releasing hormone: development of a recombinant contraceptive vaccine. Vaccine, 13(8), 753-758. https://doi.org/10.1016/0264-410x(94)00039-p