Oxygen consumption and transport dynamics in and around a zebrafish embryo: implication for maximum size and vasculogenesis

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Zebrafish embryos need oxygen for their survival. Theembryos depend on simple diffusion for their oxygensupply, as their blood vessel system is not yet functional. This consumption causes a characteristic gradient ofoxygen concentration around and inside the embryo.We used Fick’s laws of diffusion to calculate a maxi-mum possible size of embryos without a blood-vesselsystem. These predictions are based on the availableoxygen, respiration rate and permeability parameters ofthe tissue. These calculations reveal that teleost embryosdevelop a vascular system well in advance of the needfor an oxygen transport mechanism.In addition we present a numerical-experimental diffu-sion study in which we elucidate the spatial oxygen pro-file around and inside a zebrafish embryo. Oxygenmicro-electrodes were used to measure oxygen levelsaround and inside the embryo. A finite differences-basedmodel of diffusion was developed to predict the oxygenlevels. Lowest oxygen levels are found in the head witha gradient of posteriorly increasing pressure along themidline of the embryo. Model and experiment matched,proving Fick’s law to be the ruling principle behind therespiration processes inside the embryo. Furthermore,this study shows the yolk mass to have a relatively highoxygen permeability as compared to the surroundingmedium and the respiring tissue. The oxygen permea-bility of the respiring tissue in this stage is close to thatof water.Knowledge of the details of the oxygen distribution areimportant for an understanding of vasculogenesis andangiogenesis since oxygen levels influence the expres-sions of endothelial growth factors
Original languageEnglish
Title of host publicationAbstracts of the Annual Main Meeting of the Society for Experimental Biology
PagesS60-S61
Number of pages2
Volume137A
Publication statusPublished - 2004
EventAnnual Main Meeting of the Society for Experimental Biology,Edinburgh, UK -
Duration: 29 Mar 20042 Apr 2004

Publication series

NameComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
PublisherElsevier
Number3 Supplement
Volume137
ISSN (Print)1095-6433
ISSN (Electronic)1531-4332

Conference

ConferenceAnnual Main Meeting of the Society for Experimental Biology,Edinburgh, UK
Period29/03/042/04/04

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    Kranenbarg, S., van den Boogaart, J. G. M., & van Leeuwen, J. L. (2004). Oxygen consumption and transport dynamics in and around a zebrafish embryo: implication for maximum size and vasculogenesis. In Abstracts of the Annual Main Meeting of the Society for Experimental Biology (Vol. 137A, pp. S60-S61). (Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology; Vol. 137, No. 3 Supplement). https://edepot.wur.nl/526759