Oxidation of anthracene and benzo[a]pyrene by laccases from Trametes versicolor.

P.J. Collins, M.J.J. Kotterman, J.A. Field, A.D.W. Dobson

    Research output: Contribution to journalArticleAcademicpeer-review

    296 Citations (Scopus)


    The in vitro oxidation of the two polycyclic aromatic hydrocarbons anthracene and benzo[a]pyrene, which have ionization potentials of <=7.45 eV, is catalyzed by laccases from Trametes versicolor. Crude laccase preparations were able to oxidize both anthracene and the potent carcinogen benzo[a]pyrene. Oxidation of benzo[a]pyrene was enhanced by the addition of the cooxidant 2,2(prm1)-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS), while an increased anthracene oxidizing ability was observed in the presence of the low-molecular-weight culture fluid ultrafiltrate. Two purified laccase isozymes from T. versicolor were found to have similar oxidative activities towards anthracene and benzo[a]pyrene. Oxidation of anthracene by the purified isozymes was enhanced in the presence of ABTS, while ABTS was essential for the oxidation of benzo[a]pyrene. In all cases anthraquinone was identified as the major end product of anthracene oxidation. These findings indicate that laccases may have a role in the oxidation of polycyclic aromatic hydrocarbons by white rot fungi.
    Original languageEnglish
    Pages (from-to)4563-4567
    JournalApplied and Environmental Microbiology
    Publication statusPublished - 1996


    Dive into the research topics of 'Oxidation of anthracene and benzo[a]pyrene by laccases from Trametes versicolor.'. Together they form a unique fingerprint.

    Cite this