Overproduction and characterization of UvrA protein encoded by plasmid from Acetobacter estunensis GP2

Peter Grones; Silvia Mináriková-Vávrová; Martin Babič; Zuzana Odnogová; Jozef Grones

Overproduction and characterization of UvrA protein encoded by plasmid from Acetobacter estunensis GP2

Peter Grones^*, Silvia Mináriková-Vávrová, Martin Babič, Zuzana Odnogová, Jozef Grones

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Plasmid pGP2 from Acetobacter estunensis GP2 encodes 1 296 bp uvrA gene for 432 aa reparation protein exinuclease A. Bioinformatic analysis of the protein confirmed 13 α-helixes and 14 β-structures, two domains of ABC transporters, one GTPase domain and a 21-aminoacid large trans-membrane domain. The protein has 98-88 % identities with some bacterial genome-encoded proteins. uvrA gene amplified by PCR was cloned in pET28a- expression vector and purified by HPLC affinity chromato-graphy. The purified protein (47.17 kDa) shows the ATPase activity ten times lower than the Rep34 protein (isoletectric point 6.8) from plasmid pGP2.

Original language	English
Pages (from-to)	777-782
Number of pages	6
Journal	Chemicke Listy
Volume	106
Issue number	8
Publication status	Published - Aug 2012
Externally published	Yes

Cite this

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title = "Overproduction and characterization of UvrA protein encoded by plasmid from Acetobacter estunensis GP2",

abstract = "Plasmid pGP2 from Acetobacter estunensis GP2 encodes 1 296 bp uvrA gene for 432 aa reparation protein exinuclease A. Bioinformatic analysis of the protein confirmed 13 α-helixes and 14 β-structures, two domains of ABC transporters, one GTPase domain and a 21-aminoacid large trans-membrane domain. The protein has 98-88 % identities with some bacterial genome-encoded proteins. uvrA gene amplified by PCR was cloned in pET28a- expression vector and purified by HPLC affinity chromato-graphy. The purified protein (47.17 kDa) shows the ATPase activity ten times lower than the Rep34 protein (isoletectric point 6.8) from plasmid pGP2.",

author = "Peter Grones and Silvia Min{\'a}rikov{\'a}-V{\'a}vrov{\'a} and Martin Babi{\v c} and Zuzana Odnogov{\'a} and Jozef Grones",

year = "2012",

month = aug,

language = "English",

volume = "106",

pages = "777--782",

journal = "Chemicke Listy",

issn = "0009-2770",

publisher = "Czech Society of Chemical Engineering",

number = "8",

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TY - JOUR

T1 - Overproduction and characterization of UvrA protein encoded by plasmid from Acetobacter estunensis GP2

AU - Grones, Peter

AU - Mináriková-Vávrová, Silvia

AU - Babič, Martin

AU - Odnogová, Zuzana

AU - Grones, Jozef

PY - 2012/8

Y1 - 2012/8

N2 - Plasmid pGP2 from Acetobacter estunensis GP2 encodes 1 296 bp uvrA gene for 432 aa reparation protein exinuclease A. Bioinformatic analysis of the protein confirmed 13 α-helixes and 14 β-structures, two domains of ABC transporters, one GTPase domain and a 21-aminoacid large trans-membrane domain. The protein has 98-88 % identities with some bacterial genome-encoded proteins. uvrA gene amplified by PCR was cloned in pET28a- expression vector and purified by HPLC affinity chromato-graphy. The purified protein (47.17 kDa) shows the ATPase activity ten times lower than the Rep34 protein (isoletectric point 6.8) from plasmid pGP2.

AB - Plasmid pGP2 from Acetobacter estunensis GP2 encodes 1 296 bp uvrA gene for 432 aa reparation protein exinuclease A. Bioinformatic analysis of the protein confirmed 13 α-helixes and 14 β-structures, two domains of ABC transporters, one GTPase domain and a 21-aminoacid large trans-membrane domain. The protein has 98-88 % identities with some bacterial genome-encoded proteins. uvrA gene amplified by PCR was cloned in pET28a- expression vector and purified by HPLC affinity chromato-graphy. The purified protein (47.17 kDa) shows the ATPase activity ten times lower than the Rep34 protein (isoletectric point 6.8) from plasmid pGP2.

M3 - Article

AN - SCOPUS:84866932397

SN - 0009-2770

VL - 106

SP - 777

EP - 782

JO - Chemicke Listy

JF - Chemicke Listy

IS - 8

ER -

Overproduction and characterization of UvrA protein encoded by plasmid from Acetobacter estunensis GP2

Abstract

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