OSCILLATOR: A system for analysis of diurnal leaf growth using infrared photography combined with wavelet transformation

R.M.E.H. Bours, M. Muthuraman, H.J. Bouwmeester, A.R. van der Krol

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

Background Quantification of leaf movement is an important tool for characterising the effects of environmental signals and the circadian clock on plant development. Analysis of leaf movement is currently restricted by the attachment of sensors to the plant or dependent upon visible light for time-lapse photography. The study of leaf growth movement rhythms in mature plants under biological relevant conditions, e.g. diurnal light and dark conditions, is therefore problematic. Results Here we present OSCILLATOR, an affordable system for the analysis of rhythmic leaf growth movement in mature plants. The system contains three modules: (1) Infrared time-lapse imaging of growing mature plants (2) measurement of projected distances between leaf tip and plant apex (leaf tip tracking growth-curves) and (3) extraction of phase, period and amplitude of leaf growth oscillations using wavelet analysis. A proof-of-principle is provided by characterising parameters of rhythmic leaf growth movement of different Arabidopsis thaliana accessions as well as of Petunia hybrida and Solanum lycopersicum plants under diurnal conditions. The amplitude of leaf oscillations correlated to published data on leaf angles, while amplitude and leaf length did not correlate, suggesting a distinct leaf growth profile for each accession. Arabidopsis mutant accession Landsberg erecta displayed a late phase (timing of peak oscillation) compared to other accessions and this trait appears unrelated to the ERECTA locus. Conclusions OSCILLATOR is a low cost and easy to implement system that can accurately and reproducibly quantify rhythmic growth of mature plants for different species under diurnal light/dark cycling.
Original languageEnglish
Article number29
JournalPlant Methods
Volume8
DOIs
Publication statusPublished - 2012

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infrared photography
systems analysis
Photography
Systems Analysis
Growth
leaves
mature plants
Arabidopsis
Light
oscillation
Time-Lapse Imaging
Petunia
Wavelet Analysis
Circadian Clocks
Plant Leaves
Plant Development
Lycopersicon esculentum
wavelet
leaf angle
Petunia hybrida

Keywords

  • arabidopsis-thaliana
  • circadian clock
  • ethylene
  • plant
  • movement
  • mutants
  • rhythms
  • angle

Cite this

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title = "OSCILLATOR: A system for analysis of diurnal leaf growth using infrared photography combined with wavelet transformation",
abstract = "Background Quantification of leaf movement is an important tool for characterising the effects of environmental signals and the circadian clock on plant development. Analysis of leaf movement is currently restricted by the attachment of sensors to the plant or dependent upon visible light for time-lapse photography. The study of leaf growth movement rhythms in mature plants under biological relevant conditions, e.g. diurnal light and dark conditions, is therefore problematic. Results Here we present OSCILLATOR, an affordable system for the analysis of rhythmic leaf growth movement in mature plants. The system contains three modules: (1) Infrared time-lapse imaging of growing mature plants (2) measurement of projected distances between leaf tip and plant apex (leaf tip tracking growth-curves) and (3) extraction of phase, period and amplitude of leaf growth oscillations using wavelet analysis. A proof-of-principle is provided by characterising parameters of rhythmic leaf growth movement of different Arabidopsis thaliana accessions as well as of Petunia hybrida and Solanum lycopersicum plants under diurnal conditions. The amplitude of leaf oscillations correlated to published data on leaf angles, while amplitude and leaf length did not correlate, suggesting a distinct leaf growth profile for each accession. Arabidopsis mutant accession Landsberg erecta displayed a late phase (timing of peak oscillation) compared to other accessions and this trait appears unrelated to the ERECTA locus. Conclusions OSCILLATOR is a low cost and easy to implement system that can accurately and reproducibly quantify rhythmic growth of mature plants for different species under diurnal light/dark cycling.",
keywords = "arabidopsis-thaliana, circadian clock, ethylene, plant, movement, mutants, rhythms, angle",
author = "R.M.E.H. Bours and M. Muthuraman and H.J. Bouwmeester and {van der Krol}, A.R.",
year = "2012",
doi = "10.1186/1746-4811-8-29",
language = "English",
volume = "8",
journal = "Plant Methods",
issn = "1746-4811",
publisher = "Springer Verlag",

}

OSCILLATOR: A system for analysis of diurnal leaf growth using infrared photography combined with wavelet transformation. / Bours, R.M.E.H.; Muthuraman, M.; Bouwmeester, H.J.; van der Krol, A.R.

In: Plant Methods, Vol. 8, 29, 2012.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - OSCILLATOR: A system for analysis of diurnal leaf growth using infrared photography combined with wavelet transformation

AU - Bours, R.M.E.H.

AU - Muthuraman, M.

AU - Bouwmeester, H.J.

AU - van der Krol, A.R.

PY - 2012

Y1 - 2012

N2 - Background Quantification of leaf movement is an important tool for characterising the effects of environmental signals and the circadian clock on plant development. Analysis of leaf movement is currently restricted by the attachment of sensors to the plant or dependent upon visible light for time-lapse photography. The study of leaf growth movement rhythms in mature plants under biological relevant conditions, e.g. diurnal light and dark conditions, is therefore problematic. Results Here we present OSCILLATOR, an affordable system for the analysis of rhythmic leaf growth movement in mature plants. The system contains three modules: (1) Infrared time-lapse imaging of growing mature plants (2) measurement of projected distances between leaf tip and plant apex (leaf tip tracking growth-curves) and (3) extraction of phase, period and amplitude of leaf growth oscillations using wavelet analysis. A proof-of-principle is provided by characterising parameters of rhythmic leaf growth movement of different Arabidopsis thaliana accessions as well as of Petunia hybrida and Solanum lycopersicum plants under diurnal conditions. The amplitude of leaf oscillations correlated to published data on leaf angles, while amplitude and leaf length did not correlate, suggesting a distinct leaf growth profile for each accession. Arabidopsis mutant accession Landsberg erecta displayed a late phase (timing of peak oscillation) compared to other accessions and this trait appears unrelated to the ERECTA locus. Conclusions OSCILLATOR is a low cost and easy to implement system that can accurately and reproducibly quantify rhythmic growth of mature plants for different species under diurnal light/dark cycling.

AB - Background Quantification of leaf movement is an important tool for characterising the effects of environmental signals and the circadian clock on plant development. Analysis of leaf movement is currently restricted by the attachment of sensors to the plant or dependent upon visible light for time-lapse photography. The study of leaf growth movement rhythms in mature plants under biological relevant conditions, e.g. diurnal light and dark conditions, is therefore problematic. Results Here we present OSCILLATOR, an affordable system for the analysis of rhythmic leaf growth movement in mature plants. The system contains three modules: (1) Infrared time-lapse imaging of growing mature plants (2) measurement of projected distances between leaf tip and plant apex (leaf tip tracking growth-curves) and (3) extraction of phase, period and amplitude of leaf growth oscillations using wavelet analysis. A proof-of-principle is provided by characterising parameters of rhythmic leaf growth movement of different Arabidopsis thaliana accessions as well as of Petunia hybrida and Solanum lycopersicum plants under diurnal conditions. The amplitude of leaf oscillations correlated to published data on leaf angles, while amplitude and leaf length did not correlate, suggesting a distinct leaf growth profile for each accession. Arabidopsis mutant accession Landsberg erecta displayed a late phase (timing of peak oscillation) compared to other accessions and this trait appears unrelated to the ERECTA locus. Conclusions OSCILLATOR is a low cost and easy to implement system that can accurately and reproducibly quantify rhythmic growth of mature plants for different species under diurnal light/dark cycling.

KW - arabidopsis-thaliana

KW - circadian clock

KW - ethylene

KW - plant

KW - movement

KW - mutants

KW - rhythms

KW - angle

U2 - 10.1186/1746-4811-8-29

DO - 10.1186/1746-4811-8-29

M3 - Article

VL - 8

JO - Plant Methods

JF - Plant Methods

SN - 1746-4811

M1 - 29

ER -