Orthologous receptor kinases quantitatively affect the host status of barley to leaf rust fungi

Yajun Wang, Sudeep Subedi, Harmen de Vries, Pieter Doornenbal, Anton Vels, Goetz Hensel, Jochen Kumlehn, Paul A. Johnston, Xiaoquan Qi, Ikram Blilou, Rients E. Niks*, Simon G. Krattinger

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Global food security depends on cereal crops with durable disease resistance. Most cereals are colonized by rust fungi, which are pathogens of major significance for global agriculture1. Cereal rusts display a high degree of host specificity and one rust species or forma specialis generally colonizes only one cereal host2. Exploiting the non-host status and transferring non-host resistance genes between cereal crop species has been proposed as a strategy for durable rust resistance breeding. The molecular determinants that define the host status to rusts, however, are largely unknown. Here, we show that orthologous genes at the Rphq2 locus for quantitative leaf rust resistance from cultivated barley3 and Rph22 from wild bulbous barley4 affect the host status to leaf rusts. Both genes encode lectin receptor-like kinases. We transformed Rphq2 and Rph22 into an experimental barley line that has been bred for susceptibility to non-adapted leaf rusts, which allowed us to quantify resistance responses against various leaf rust species. Rphq2 conferred a much stronger resistance to the leaf rust of wild bulbous barley than to the leaf rust adapted to cultivated barley, while for Rph22 the reverse was observed. We hypothesize that adapted leaf rust species mitigate perception by cognate host receptors by lowering ligand recognition. Our results provide an example of orthologous genes that connect the quantitative host with non-host resistance to cereal rusts. Such genes provide a basis to exploit non-host resistance in molecular breeding.

Original languageEnglish
Pages (from-to)1129-1135
Number of pages7
JournalNature Plants
Volume5
Issue number11
DOIs
Publication statusPublished - 11 Nov 2019

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leaf rust
phosphotransferases (kinases)
barley
receptors
fungi
grain crops
genes
Hordeum bulbosum
breeding
host specificity
food security
lectins
disease resistance
breeds
loci
pathogens

Cite this

Wang, Y., Subedi, S., de Vries, H., Doornenbal, P., Vels, A., Hensel, G., ... Krattinger, S. G. (2019). Orthologous receptor kinases quantitatively affect the host status of barley to leaf rust fungi. Nature Plants, 5(11), 1129-1135. https://doi.org/10.1038/s41477-019-0545-2
Wang, Yajun ; Subedi, Sudeep ; de Vries, Harmen ; Doornenbal, Pieter ; Vels, Anton ; Hensel, Goetz ; Kumlehn, Jochen ; Johnston, Paul A. ; Qi, Xiaoquan ; Blilou, Ikram ; Niks, Rients E. ; Krattinger, Simon G. / Orthologous receptor kinases quantitatively affect the host status of barley to leaf rust fungi. In: Nature Plants. 2019 ; Vol. 5, No. 11. pp. 1129-1135.
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abstract = "Global food security depends on cereal crops with durable disease resistance. Most cereals are colonized by rust fungi, which are pathogens of major significance for global agriculture1. Cereal rusts display a high degree of host specificity and one rust species or forma specialis generally colonizes only one cereal host2. Exploiting the non-host status and transferring non-host resistance genes between cereal crop species has been proposed as a strategy for durable rust resistance breeding. The molecular determinants that define the host status to rusts, however, are largely unknown. Here, we show that orthologous genes at the Rphq2 locus for quantitative leaf rust resistance from cultivated barley3 and Rph22 from wild bulbous barley4 affect the host status to leaf rusts. Both genes encode lectin receptor-like kinases. We transformed Rphq2 and Rph22 into an experimental barley line that has been bred for susceptibility to non-adapted leaf rusts, which allowed us to quantify resistance responses against various leaf rust species. Rphq2 conferred a much stronger resistance to the leaf rust of wild bulbous barley than to the leaf rust adapted to cultivated barley, while for Rph22 the reverse was observed. We hypothesize that adapted leaf rust species mitigate perception by cognate host receptors by lowering ligand recognition. Our results provide an example of orthologous genes that connect the quantitative host with non-host resistance to cereal rusts. Such genes provide a basis to exploit non-host resistance in molecular breeding.",
author = "Yajun Wang and Sudeep Subedi and {de Vries}, Harmen and Pieter Doornenbal and Anton Vels and Goetz Hensel and Jochen Kumlehn and Johnston, {Paul A.} and Xiaoquan Qi and Ikram Blilou and Niks, {Rients E.} and Krattinger, {Simon G.}",
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Wang, Y, Subedi, S, de Vries, H, Doornenbal, P, Vels, A, Hensel, G, Kumlehn, J, Johnston, PA, Qi, X, Blilou, I, Niks, RE & Krattinger, SG 2019, 'Orthologous receptor kinases quantitatively affect the host status of barley to leaf rust fungi', Nature Plants, vol. 5, no. 11, pp. 1129-1135. https://doi.org/10.1038/s41477-019-0545-2

Orthologous receptor kinases quantitatively affect the host status of barley to leaf rust fungi. / Wang, Yajun; Subedi, Sudeep; de Vries, Harmen; Doornenbal, Pieter; Vels, Anton; Hensel, Goetz; Kumlehn, Jochen; Johnston, Paul A.; Qi, Xiaoquan; Blilou, Ikram; Niks, Rients E.; Krattinger, Simon G.

In: Nature Plants, Vol. 5, No. 11, 11.11.2019, p. 1129-1135.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Orthologous receptor kinases quantitatively affect the host status of barley to leaf rust fungi

AU - Wang, Yajun

AU - Subedi, Sudeep

AU - de Vries, Harmen

AU - Doornenbal, Pieter

AU - Vels, Anton

AU - Hensel, Goetz

AU - Kumlehn, Jochen

AU - Johnston, Paul A.

AU - Qi, Xiaoquan

AU - Blilou, Ikram

AU - Niks, Rients E.

AU - Krattinger, Simon G.

PY - 2019/11/11

Y1 - 2019/11/11

N2 - Global food security depends on cereal crops with durable disease resistance. Most cereals are colonized by rust fungi, which are pathogens of major significance for global agriculture1. Cereal rusts display a high degree of host specificity and one rust species or forma specialis generally colonizes only one cereal host2. Exploiting the non-host status and transferring non-host resistance genes between cereal crop species has been proposed as a strategy for durable rust resistance breeding. The molecular determinants that define the host status to rusts, however, are largely unknown. Here, we show that orthologous genes at the Rphq2 locus for quantitative leaf rust resistance from cultivated barley3 and Rph22 from wild bulbous barley4 affect the host status to leaf rusts. Both genes encode lectin receptor-like kinases. We transformed Rphq2 and Rph22 into an experimental barley line that has been bred for susceptibility to non-adapted leaf rusts, which allowed us to quantify resistance responses against various leaf rust species. Rphq2 conferred a much stronger resistance to the leaf rust of wild bulbous barley than to the leaf rust adapted to cultivated barley, while for Rph22 the reverse was observed. We hypothesize that adapted leaf rust species mitigate perception by cognate host receptors by lowering ligand recognition. Our results provide an example of orthologous genes that connect the quantitative host with non-host resistance to cereal rusts. Such genes provide a basis to exploit non-host resistance in molecular breeding.

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U2 - 10.1038/s41477-019-0545-2

DO - 10.1038/s41477-019-0545-2

M3 - Article

VL - 5

SP - 1129

EP - 1135

JO - Nature Plants

JF - Nature Plants

SN - 2055-026X

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ER -