Orthogonal Functionalization of Ferritin via Supramolecular Re-Assembly

Silvia J. Spa, Anton Bunschoten, Marcus T.M. Rood, R.J.B. Peters, Abraham J. Koster, F.W.B. Van Leeuwen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)


To investigate if the degree of functionalization of ferritin could be controlled using a supramolecular self-assembly process, two photophysical separable batches of ferritin were created by functionalizing ferritin capsids with either Cy3- or Cy5-dye (loading rate of about 50 %). After dis-assembly, Cy3-, Cy5- as well as non-functionalized ferritin subunits were mixed in variable ratios. Photophysical measurements revealed that the ratio in which the subunits were mixed was indeed indicative for the ratios in which the functionalized subunits were observed in the re-assembled capsids. During re-assembly, however, a slight preference for the inclusion of non-functionalized subunits was observed, indicating the reactivity decreased following functionalization. The iron biomineralization properties of ferritin were retained by the multi-functionalized capsids as FeII diffused rapidly inside making it visible by transmission electron microscopy (TEM). These combined data indicate that it is possible to functionalize ferritin in an orthogonal manner using the supramolecular interaction between ferritin subunits.

Original languageEnglish
Pages (from-to)4603-4610
JournalEuropean Journal of Inorganic Chemistry
Issue number27
Publication statusPublished - 2015


  • Ferritin
  • Insertion
  • Iron
  • Nanoparticles
  • Self-assembly
  • Supramolecular chemistry


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