Orientation of Heterorhabditis megidis to insect hosts and plant roots in a Y-tube olfactometer

F.C. Zoon, M.I.C. Boff, P.H. Smits

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    54 Citations (Scopus)


    The host-searching behaviour of Heterorhabditis megidis strain NLH-E 87.3 in the presence of insect hosts and plant roots, offered individually and in combination, was studied using a newly developed Y-tube olfactometer filled with sand. Within a period of 24 hours infective juveniles (IJs) were significantly attracted to living G, mellonella larvae and caused 100% larval mortality. Otiorhynchus sulcatus larvae, however, did not elicit host-oriented movement of IJs and no larval mortality was observed. Roots of strawberry plants induced a negative response in IJs. The combination of strawberry roots and O. sulcatus larvae, however, strongly attracted IJs leading to 37% host mortality. It was shown that this type of Y-tube choice arena is a useful tool in studying the searching behaviour of entomopathogenic nematodes in a semi-natural habitat
    Original languageEnglish
    Pages (from-to)329-337
    JournalEntomologia Experimentalis et Applicata
    Publication statusPublished - 2001

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