Oriental lily hybrids engineered to resist aphid attack

    Research output: Contribution to journalArticleAcademicpeer-review

    8 Citations (Scopus)

    Abstract

    Establishing in vitro bulb scale cultures of lily cultivars followed by callusing and regeneration after gene transfer was found to be not very successful in our hands, except for ‘Snow Queen’. Identifying a more generally applicable system to generate callus with the ability to regenerate and amenable to Agrobacterium-mediated gene transfer was the goal of the research described here. Callus was induced on style and filament explants of 26 cultivars of lily. The cultivars were chosen from the hybrid groups longiflorum, asiatics and orientals of the genus Lilium but also interspecific hybrids were represented. Most cultivars were diploids but some were of triploid level and one was tetraploid. In general once callus was induced, it could relatively easily be maintained and propagated for further use. Regeneration was observed on both callus types from all cultivars tested with an efficiency ranging from 40 to 100%. Gene transfer as demonstrated by positive reporter gene uidA activity was found in all cultivars tested. Transgenic plants could be obtained in the first series of transformations and the applicability of a marker-free system was proven in lily. The protocol can now be used for the introduction of genes aiming at conferring resistance to aphids.
    Original languageEnglish
    Pages (from-to)253-257
    JournalActa Horticulturae
    Volume2009
    Issue number836
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    Lilium
    Aphidoidea
    callus
    cultivars
    gene transfer
    triploidy
    Agrobacterium
    reporter genes
    snow
    bulbs
    tetraploidy
    transgenic plants
    explants
    diploidy
    genes

    Cite this

    @article{6181408a1735440baab0562732e2d57d,
    title = "Oriental lily hybrids engineered to resist aphid attack",
    abstract = "Establishing in vitro bulb scale cultures of lily cultivars followed by callusing and regeneration after gene transfer was found to be not very successful in our hands, except for ‘Snow Queen’. Identifying a more generally applicable system to generate callus with the ability to regenerate and amenable to Agrobacterium-mediated gene transfer was the goal of the research described here. Callus was induced on style and filament explants of 26 cultivars of lily. The cultivars were chosen from the hybrid groups longiflorum, asiatics and orientals of the genus Lilium but also interspecific hybrids were represented. Most cultivars were diploids but some were of triploid level and one was tetraploid. In general once callus was induced, it could relatively easily be maintained and propagated for further use. Regeneration was observed on both callus types from all cultivars tested with an efficiency ranging from 40 to 100{\%}. Gene transfer as demonstrated by positive reporter gene uidA activity was found in all cultivars tested. Transgenic plants could be obtained in the first series of transformations and the applicability of a marker-free system was proven in lily. The protocol can now be used for the introduction of genes aiming at conferring resistance to aphids.",
    author = "F.A. Krens and T.R. Menzel and L. Chang and D.C.T. Dees and {van Kronenburg}, B.C.E.",
    year = "2009",
    doi = "10.17660/ActaHortic.2009.836.36",
    language = "English",
    volume = "2009",
    pages = "253--257",
    journal = "Acta Horticulturae",
    issn = "0567-7572",
    publisher = "International Society for Horticultural Science",
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    }

    Oriental lily hybrids engineered to resist aphid attack. / Krens, F.A.; Menzel, T.R.; Chang, L.; Dees, D.C.T.; van Kronenburg, B.C.E.

    In: Acta Horticulturae, Vol. 2009, No. 836, 2009, p. 253-257.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Oriental lily hybrids engineered to resist aphid attack

    AU - Krens, F.A.

    AU - Menzel, T.R.

    AU - Chang, L.

    AU - Dees, D.C.T.

    AU - van Kronenburg, B.C.E.

    PY - 2009

    Y1 - 2009

    N2 - Establishing in vitro bulb scale cultures of lily cultivars followed by callusing and regeneration after gene transfer was found to be not very successful in our hands, except for ‘Snow Queen’. Identifying a more generally applicable system to generate callus with the ability to regenerate and amenable to Agrobacterium-mediated gene transfer was the goal of the research described here. Callus was induced on style and filament explants of 26 cultivars of lily. The cultivars were chosen from the hybrid groups longiflorum, asiatics and orientals of the genus Lilium but also interspecific hybrids were represented. Most cultivars were diploids but some were of triploid level and one was tetraploid. In general once callus was induced, it could relatively easily be maintained and propagated for further use. Regeneration was observed on both callus types from all cultivars tested with an efficiency ranging from 40 to 100%. Gene transfer as demonstrated by positive reporter gene uidA activity was found in all cultivars tested. Transgenic plants could be obtained in the first series of transformations and the applicability of a marker-free system was proven in lily. The protocol can now be used for the introduction of genes aiming at conferring resistance to aphids.

    AB - Establishing in vitro bulb scale cultures of lily cultivars followed by callusing and regeneration after gene transfer was found to be not very successful in our hands, except for ‘Snow Queen’. Identifying a more generally applicable system to generate callus with the ability to regenerate and amenable to Agrobacterium-mediated gene transfer was the goal of the research described here. Callus was induced on style and filament explants of 26 cultivars of lily. The cultivars were chosen from the hybrid groups longiflorum, asiatics and orientals of the genus Lilium but also interspecific hybrids were represented. Most cultivars were diploids but some were of triploid level and one was tetraploid. In general once callus was induced, it could relatively easily be maintained and propagated for further use. Regeneration was observed on both callus types from all cultivars tested with an efficiency ranging from 40 to 100%. Gene transfer as demonstrated by positive reporter gene uidA activity was found in all cultivars tested. Transgenic plants could be obtained in the first series of transformations and the applicability of a marker-free system was proven in lily. The protocol can now be used for the introduction of genes aiming at conferring resistance to aphids.

    U2 - 10.17660/ActaHortic.2009.836.36

    DO - 10.17660/ActaHortic.2009.836.36

    M3 - Article

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    SP - 253

    EP - 257

    JO - Acta Horticulturae

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