Organizer-Derived WOX5 Signal Maintains Root Columella Stem Cells through Chromatin-Mediated Repression of CDF4 Expression.

L. Pi, E. van der Graaff, C.I. Llavata Peris, D. Weijers, L. Henning, E. de Groot, T. Laux

Research output: Contribution to journalArticleAcademicpeer-review

99 Citations (Scopus)

Abstract

Stem cells in plants and animals are maintained pluripotent by signals from adjacent niche cells. In plants, WUSCHEL HOMEOBOX (WOX) transcription factors are central regulators of stem cell maintenance in different meristem types, yet their molecular mode of action has remained elusive. Here we show that in the Arabidopsis root meristem, the WOX5 protein moves from the root niche organizer, the quiescent center, into the columella stem cells, where it directly represses the transcription factor gene CDF4. This creates a gradient of CDF4 transcription, which promotes differentiation opposite to the WOX5 gradient, allowing stem cell daughter cells to exit the stem cell state. We further show that WOX5 represses CDF4 transcription by recruiting TPL/TPR co-repressors and the histone deacetylase HDA19, which consequently induces histone deacetylation at the CDF4 regulatory region. Our results show that chromatin-mediated repression of differentiation programs is a common strategy in plant and animal stem cell niches.
LanguageEnglish
Pages576-588
JournalDevelopmental Cell
Volume33
Issue number5
DOIs
Publication statusPublished - 2015

Fingerprint

Stem cells
Chromatin
Stem Cells
Meristem
Transcription
Transcription Factors
Plant Stems
Animals
Stem Cell Niche
Co-Repressor Proteins
Histone Deacetylases
Nucleic Acid Regulatory Sequences
Arabidopsis
Histones
Maintenance
Genes
Proteins

Keywords

  • histone deacetylase
  • arabidopsis-thaliana
  • transcriptional repression
  • gene-expression
  • wuschel
  • meristem
  • shoot
  • topless
  • protein
  • fate

Cite this

@article{21f72e2fb31f4a5bbbafa0949d0c1b7c,
title = "Organizer-Derived WOX5 Signal Maintains Root Columella Stem Cells through Chromatin-Mediated Repression of CDF4 Expression.",
abstract = "Stem cells in plants and animals are maintained pluripotent by signals from adjacent niche cells. In plants, WUSCHEL HOMEOBOX (WOX) transcription factors are central regulators of stem cell maintenance in different meristem types, yet their molecular mode of action has remained elusive. Here we show that in the Arabidopsis root meristem, the WOX5 protein moves from the root niche organizer, the quiescent center, into the columella stem cells, where it directly represses the transcription factor gene CDF4. This creates a gradient of CDF4 transcription, which promotes differentiation opposite to the WOX5 gradient, allowing stem cell daughter cells to exit the stem cell state. We further show that WOX5 represses CDF4 transcription by recruiting TPL/TPR co-repressors and the histone deacetylase HDA19, which consequently induces histone deacetylation at the CDF4 regulatory region. Our results show that chromatin-mediated repression of differentiation programs is a common strategy in plant and animal stem cell niches.",
keywords = "histone deacetylase, arabidopsis-thaliana, transcriptional repression, gene-expression, wuschel, meristem, shoot, topless, protein, fate",
author = "L. Pi and {van der Graaff}, E. and {Llavata Peris}, C.I. and D. Weijers and L. Henning and {de Groot}, E. and T. Laux",
year = "2015",
doi = "10.1016/j.devcel.2015.04.024",
language = "English",
volume = "33",
pages = "576--588",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Elsevier",
number = "5",

}

Organizer-Derived WOX5 Signal Maintains Root Columella Stem Cells through Chromatin-Mediated Repression of CDF4 Expression. / Pi, L.; van der Graaff, E.; Llavata Peris, C.I.; Weijers, D.; Henning, L.; de Groot, E.; Laux, T.

In: Developmental Cell, Vol. 33, No. 5, 2015, p. 576-588.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Organizer-Derived WOX5 Signal Maintains Root Columella Stem Cells through Chromatin-Mediated Repression of CDF4 Expression.

AU - Pi, L.

AU - van der Graaff, E.

AU - Llavata Peris, C.I.

AU - Weijers, D.

AU - Henning, L.

AU - de Groot, E.

AU - Laux, T.

PY - 2015

Y1 - 2015

N2 - Stem cells in plants and animals are maintained pluripotent by signals from adjacent niche cells. In plants, WUSCHEL HOMEOBOX (WOX) transcription factors are central regulators of stem cell maintenance in different meristem types, yet their molecular mode of action has remained elusive. Here we show that in the Arabidopsis root meristem, the WOX5 protein moves from the root niche organizer, the quiescent center, into the columella stem cells, where it directly represses the transcription factor gene CDF4. This creates a gradient of CDF4 transcription, which promotes differentiation opposite to the WOX5 gradient, allowing stem cell daughter cells to exit the stem cell state. We further show that WOX5 represses CDF4 transcription by recruiting TPL/TPR co-repressors and the histone deacetylase HDA19, which consequently induces histone deacetylation at the CDF4 regulatory region. Our results show that chromatin-mediated repression of differentiation programs is a common strategy in plant and animal stem cell niches.

AB - Stem cells in plants and animals are maintained pluripotent by signals from adjacent niche cells. In plants, WUSCHEL HOMEOBOX (WOX) transcription factors are central regulators of stem cell maintenance in different meristem types, yet their molecular mode of action has remained elusive. Here we show that in the Arabidopsis root meristem, the WOX5 protein moves from the root niche organizer, the quiescent center, into the columella stem cells, where it directly represses the transcription factor gene CDF4. This creates a gradient of CDF4 transcription, which promotes differentiation opposite to the WOX5 gradient, allowing stem cell daughter cells to exit the stem cell state. We further show that WOX5 represses CDF4 transcription by recruiting TPL/TPR co-repressors and the histone deacetylase HDA19, which consequently induces histone deacetylation at the CDF4 regulatory region. Our results show that chromatin-mediated repression of differentiation programs is a common strategy in plant and animal stem cell niches.

KW - histone deacetylase

KW - arabidopsis-thaliana

KW - transcriptional repression

KW - gene-expression

KW - wuschel

KW - meristem

KW - shoot

KW - topless

KW - protein

KW - fate

U2 - 10.1016/j.devcel.2015.04.024

DO - 10.1016/j.devcel.2015.04.024

M3 - Article

VL - 33

SP - 576

EP - 588

JO - Developmental Cell

T2 - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 5

ER -