Oral cholera vaccination promotes homing of IgA+ memory B cells to the large intestine and the respiratory tract

M. van Splunter, E. van Hoffen, E.G. Floris-Vollenbroek, H. Timmerman, E.L. van de Bos, B. Meijer, L.H. Ulfman, B. Witteman, J.M. Wells, S. Brugman, H.F.J. Savelkoul, R.J.J. van Neerven*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Oral cholera vaccination is used to induce immune responses in the intestines to protect against cholera infection. However, oral vaccination may also affect immune responses in other mucosal tissues. To study this, tissue-specific homing potential and kinetics of B-cell responses were characterized after oral cholera vaccination. Healthy adult volunteers received two doses of Dukoral® and blood, saliva, nasal wash, and fecal samples were collected over time to detect vaccine-specific antibodies. Additionally, homing potential of lymphocytes to small intestine, colon, airways, skin, and periphery was measured by expression of Integrin β1 and β7, CCR9, CCR10, CCR7, and CLA. After vaccination, antibody responses to cholera toxin B (CTB) and Dukoral® were detected in serum and nasal wash. CTB-specific memory B cells in peripheral blood and tissue homing profiles of memory B cells peaked at day 18. IgA+ memory B cells expressed markers that enable homing to the airways and colon, while IgA− memory B cells primarily expressed small-intestine-homing markers. These data show that oral cholera vaccination has a differential effect on immune responses in various mucosal sites, including the respiratory tract.
Original languageEnglish
Pages (from-to)1254-1264
JournalMucosal Immunology
Volume11
Issue number4
Early online date21 Feb 2018
DOIs
Publication statusPublished - 2018

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Cholera
Large Intestine
Respiratory System
Immunoglobulin A
Vaccination
B-Lymphocytes
Cholera Toxin
Nose
Small Intestine
Colon
Saliva
Integrins
Intestines
Antibody Formation
Healthy Volunteers
Mucous Membrane
Vaccines
Lymphocytes
Skin
Antibodies

Cite this

van Splunter, M., van Hoffen, E., Floris-Vollenbroek, E. G., Timmerman, H., van de Bos, E. L., Meijer, B., ... van Neerven, R. J. J. (2018). Oral cholera vaccination promotes homing of IgA+ memory B cells to the large intestine and the respiratory tract. Mucosal Immunology, 11(4), 1254-1264. https://doi.org/10.1038/s41385-018-0006-7
van Splunter, M. ; van Hoffen, E. ; Floris-Vollenbroek, E.G. ; Timmerman, H. ; van de Bos, E.L. ; Meijer, B. ; Ulfman, L.H. ; Witteman, B. ; Wells, J.M. ; Brugman, S. ; Savelkoul, H.F.J. ; van Neerven, R.J.J. / Oral cholera vaccination promotes homing of IgA+ memory B cells to the large intestine and the respiratory tract. In: Mucosal Immunology. 2018 ; Vol. 11, No. 4. pp. 1254-1264.
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abstract = "Oral cholera vaccination is used to induce immune responses in the intestines to protect against cholera infection. However, oral vaccination may also affect immune responses in other mucosal tissues. To study this, tissue-specific homing potential and kinetics of B-cell responses were characterized after oral cholera vaccination. Healthy adult volunteers received two doses of Dukoral{\circledR} and blood, saliva, nasal wash, and fecal samples were collected over time to detect vaccine-specific antibodies. Additionally, homing potential of lymphocytes to small intestine, colon, airways, skin, and periphery was measured by expression of Integrin β1 and β7, CCR9, CCR10, CCR7, and CLA. After vaccination, antibody responses to cholera toxin B (CTB) and Dukoral{\circledR} were detected in serum and nasal wash. CTB-specific memory B cells in peripheral blood and tissue homing profiles of memory B cells peaked at day 18. IgA+ memory B cells expressed markers that enable homing to the airways and colon, while IgA− memory B cells primarily expressed small-intestine-homing markers. These data show that oral cholera vaccination has a differential effect on immune responses in various mucosal sites, including the respiratory tract.",
author = "{van Splunter}, M. and {van Hoffen}, E. and E.G. Floris-Vollenbroek and H. Timmerman and {van de Bos}, E.L. and B. Meijer and L.H. Ulfman and B. Witteman and J.M. Wells and S. Brugman and H.F.J. Savelkoul and {van Neerven}, R.J.J.",
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Oral cholera vaccination promotes homing of IgA+ memory B cells to the large intestine and the respiratory tract. / van Splunter, M.; van Hoffen, E.; Floris-Vollenbroek, E.G.; Timmerman, H.; van de Bos, E.L.; Meijer, B.; Ulfman, L.H.; Witteman, B.; Wells, J.M.; Brugman, S.; Savelkoul, H.F.J.; van Neerven, R.J.J.

In: Mucosal Immunology, Vol. 11, No. 4, 2018, p. 1254-1264.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - van Splunter, M.

AU - van Hoffen, E.

AU - Floris-Vollenbroek, E.G.

AU - Timmerman, H.

AU - van de Bos, E.L.

AU - Meijer, B.

AU - Ulfman, L.H.

AU - Witteman, B.

AU - Wells, J.M.

AU - Brugman, S.

AU - Savelkoul, H.F.J.

AU - van Neerven, R.J.J.

PY - 2018

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N2 - Oral cholera vaccination is used to induce immune responses in the intestines to protect against cholera infection. However, oral vaccination may also affect immune responses in other mucosal tissues. To study this, tissue-specific homing potential and kinetics of B-cell responses were characterized after oral cholera vaccination. Healthy adult volunteers received two doses of Dukoral® and blood, saliva, nasal wash, and fecal samples were collected over time to detect vaccine-specific antibodies. Additionally, homing potential of lymphocytes to small intestine, colon, airways, skin, and periphery was measured by expression of Integrin β1 and β7, CCR9, CCR10, CCR7, and CLA. After vaccination, antibody responses to cholera toxin B (CTB) and Dukoral® were detected in serum and nasal wash. CTB-specific memory B cells in peripheral blood and tissue homing profiles of memory B cells peaked at day 18. IgA+ memory B cells expressed markers that enable homing to the airways and colon, while IgA− memory B cells primarily expressed small-intestine-homing markers. These data show that oral cholera vaccination has a differential effect on immune responses in various mucosal sites, including the respiratory tract.

AB - Oral cholera vaccination is used to induce immune responses in the intestines to protect against cholera infection. However, oral vaccination may also affect immune responses in other mucosal tissues. To study this, tissue-specific homing potential and kinetics of B-cell responses were characterized after oral cholera vaccination. Healthy adult volunteers received two doses of Dukoral® and blood, saliva, nasal wash, and fecal samples were collected over time to detect vaccine-specific antibodies. Additionally, homing potential of lymphocytes to small intestine, colon, airways, skin, and periphery was measured by expression of Integrin β1 and β7, CCR9, CCR10, CCR7, and CLA. After vaccination, antibody responses to cholera toxin B (CTB) and Dukoral® were detected in serum and nasal wash. CTB-specific memory B cells in peripheral blood and tissue homing profiles of memory B cells peaked at day 18. IgA+ memory B cells expressed markers that enable homing to the airways and colon, while IgA− memory B cells primarily expressed small-intestine-homing markers. These data show that oral cholera vaccination has a differential effect on immune responses in various mucosal sites, including the respiratory tract.

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van Splunter M, van Hoffen E, Floris-Vollenbroek EG, Timmerman H, van de Bos EL, Meijer B et al. Oral cholera vaccination promotes homing of IgA+ memory B cells to the large intestine and the respiratory tract. Mucosal Immunology. 2018;11(4):1254-1264. https://doi.org/10.1038/s41385-018-0006-7