Optimization of Production and Downstream Processing of the Almond ß-Glucosidase-Mediated Glucosylation of Glycerol

B.M. de Roode, S.W.P.G. Peters, M.C.R. Franssen, A. van der Padt, Æ. de Groot, R.M. Boom

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

This article describes the synthesis of glyceryl glucoside from glycerol and glucose with almond β-glucosidase as the catalyst. A yield of 54ø0.45 mmol/g) was obtained. The influence of the enzyme stability, the water concentration, and the water activity on the glucoside yield were determined. A molar fraction-based equilibrium constant of 2.4 ± 0.6 was found, with which the glucoside yield could be calculated for all possible combinations of initial substrate and water fractions in the reaction mixture. A model was used to optimize the glucoside yield while minimizing one of the substrate concentrations at equilibrium. This straightforward model gives a good prediction of the measured glucoside yield, according to a parity plot.
Original languageEnglish
Pages (from-to)568-572
JournalBiotechnology and Bioengineering
Volume72
Issue number5
DOIs
Publication statusPublished - 2001

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