This article describes the synthesis of glyceryl glucoside from glycerol and glucose with almond β-glucosidase as the catalyst. A yield of 54ø0.45 mmol/g) was obtained. The influence of the enzyme stability, the water concentration, and the water activity on the glucoside yield were determined. A molar fraction-based equilibrium constant of 2.4 ± 0.6 was found, with which the glucoside yield could be calculated for all possible combinations of initial substrate and water fractions in the reaction mixture. A model was used to optimize the glucoside yield while minimizing one of the substrate concentrations at equilibrium. This straightforward model gives a good prediction of the measured glucoside yield, according to a parity plot.
|Journal||Biotechnology and Bioengineering|
|Publication status||Published - 2001|