Abstract
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of the four major enterolignan precursors [secoisolariciresinol, matairesinol, lariciresinol, and pinoresinol] in foods. The method consists of alkaline methanolic extraction, followed by enzymatic hydrolysis using Helix pomatia (H. pomatia) -glucuronidase/sulfatase. H. pomatia was selected from several enzymes based on its ability to hydrolyze isolated lignan glucosides. After ether extraction samples were analyzed and quantified against secoisolariciresinol-d8 and matairesinol-d6. The method was optimized using model products: broccoli, bread, flaxseed, and tea. The yield of methanolic extraction increased up to 81°when it was combined with alkaline hydrolysis. Detection limits were 4-10 g/(100 g dry weight) for solid foods and 0.2-0.4 g/(100 mL) for beverages. Within- and between-run coefficients of variation were 6-21 and 6-33°respectively. Recovery of lignans added to model products was satisfactory (73-123) except for matairesinol added to bread (51-55)
Original language | English |
---|---|
Pages (from-to) | 4643-4651 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 52 |
Issue number | 15 |
DOIs | |
Publication status | Published - 2004 |
Keywords
- hormone-binding globulin
- electrode array detection
- breast-cancer risk
- serum concentrations
- flax seed
- urinary-excretion
- enterolactone
- phytoestrogens
- metabolism
- isoflavonoids