Optimization of a liquid chromatography-tandem mass spectrometry method for quantification of the plant lignans secoisolariciresinol, matairesinol, lariciresinol and pinoresinol in foods

I.E.J. Milder, I.C.W. Arts, D.P. Venema, J.J.P. Lasaroms, K. Wähälä, P.C.H. Hollman

Research output: Contribution to journalArticleAcademicpeer-review

99 Citations (Scopus)

Abstract

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of the four major enterolignan precursors [secoisolariciresinol, matairesinol, lariciresinol, and pinoresinol] in foods. The method consists of alkaline methanolic extraction, followed by enzymatic hydrolysis using Helix pomatia (H. pomatia) -glucuronidase/sulfatase. H. pomatia was selected from several enzymes based on its ability to hydrolyze isolated lignan glucosides. After ether extraction samples were analyzed and quantified against secoisolariciresinol-d8 and matairesinol-d6. The method was optimized using model products: broccoli, bread, flaxseed, and tea. The yield of methanolic extraction increased up to 81°when it was combined with alkaline hydrolysis. Detection limits were 4-10 g/(100 g dry weight) for solid foods and 0.2-0.4 g/(100 mL) for beverages. Within- and between-run coefficients of variation were 6-21 and 6-33°respectively. Recovery of lignans added to model products was satisfactory (73-123) except for matairesinol added to bread (51-55)
Original languageEnglish
Pages (from-to)4643-4651
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number15
DOIs
Publication statusPublished - 2004

Keywords

  • hormone-binding globulin
  • electrode array detection
  • breast-cancer risk
  • serum concentrations
  • flax seed
  • urinary-excretion
  • enterolactone
  • phytoestrogens
  • metabolism
  • isoflavonoids

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