Optimising single cell activity assessment of Lactobacillus plantarum by fluorescent in situ hybridisation as affected by growth

M.C. de Vries, E.E. Vaughan, M. Kleerebezem, W.M. de Vos

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

Fluorescent in situ hybridisation (FISH) with a 16S ribosomal RNA (rRNA)-targeted oligonucleotide probe, Eub338, could be used to estimate the in situ activity of Lactobacillus plantarum WCFS1 in exponentially growing cells. However, L. plantarum is capable of growth to very high cell densities, and the properties of the L. plantarum cell envelope prevented effective entry of the fluorescent oligonucleotide probe into the cells at later stages of growth at high cell densities. Total rRNA measurements of cells isolated at different growth stages showed maximal amounts of RNA (8.77 +/- 0.8 fg) per cell at the early stationary phase and confirmed the effectiveness of FISH for accurate activity measurement in exponentially growing cells. (C) 2004 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)109-115
JournalJournal of Microbiological Methods
Volume59
Issue number1
DOIs
Publication statusPublished - 2004

Keywords

  • ribosomal-rna
  • salmonella-typhimurium
  • messenger-rna
  • protein

Fingerprint Dive into the research topics of 'Optimising single cell activity assessment of Lactobacillus plantarum by fluorescent in situ hybridisation as affected by growth'. Together they form a unique fingerprint.

Cite this