Optimal conditions for enhanced β-mannanase production by recombinant Aspergillus sojae

Bengu Ozturk; Deniz Cekmecelioglu; Zumrut Begum Ogel

doi:10.1016/j.molcatb.2010.02.009

Optimal conditions for enhanced β-mannanase production by recombinant Aspergillus sojae

Bengu Ozturk^*, Deniz Cekmecelioglu, Zumrut Begum Ogel

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

35 Citations (Scopus)

Abstract

Optimization of the growth conditions for maximum β-mannanase production in shake flasks by using recombinant Aspergillus sojae ATCC11906 (AsT1) was carried out by Box-Behnken design of response surface methodology. The highest β-mannanase activity on the fourth day of cultivation at 30 °C was obtained as 363 U/ml in the optimized medium consisting of 7% sugar beet molasses, 0.43% NH4NO3, 0.1% K2HPO4 and 0.05% MgSO4 (by weight per volume) at 207 rpm. On the sixth day of cultivation under the optimized conditions, the highest β-mannanase activity was achieved as 482 U/ml which is 1.4-fold of 352 U/ml activity found on glucose medium previously.

Original language	English
Pages (from-to)	135-139
Number of pages	5
Journal	Journal of Molecular Catalysis B: Enzymatic
Volume	64
Issue number	3-4
DOIs	https://doi.org/10.1016/j.molcatb.2010.02.009
Publication status	Published - Jul 2010
Externally published	Yes

Keywords

Box-Behnken
Endo-β-1,4-mannanase
Recombinant Aspergillus sojae
Response surface methodology

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10.1016/j.molcatb.2010.02.009

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title = "Optimal conditions for enhanced β-mannanase production by recombinant Aspergillus sojae",

abstract = "Optimization of the growth conditions for maximum β-mannanase production in shake flasks by using recombinant Aspergillus sojae ATCC11906 (AsT1) was carried out by Box-Behnken design of response surface methodology. The highest β-mannanase activity on the fourth day of cultivation at 30 °C was obtained as 363 U/ml in the optimized medium consisting of 7% sugar beet molasses, 0.43% NH4NO3, 0.1% K2HPO4 and 0.05% MgSO4 (by weight per volume) at 207 rpm. On the sixth day of cultivation under the optimized conditions, the highest β-mannanase activity was achieved as 482 U/ml which is 1.4-fold of 352 U/ml activity found on glucose medium previously.",

keywords = "Box-Behnken, Endo-β-1,4-mannanase, Recombinant Aspergillus sojae, Response surface methodology",

author = "Bengu Ozturk and Deniz Cekmecelioglu and Ogel, {Zumrut Begum}",

year = "2010",

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doi = "10.1016/j.molcatb.2010.02.009",

language = "English",

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TY - JOUR

T1 - Optimal conditions for enhanced β-mannanase production by recombinant Aspergillus sojae

AU - Ozturk, Bengu

AU - Cekmecelioglu, Deniz

AU - Ogel, Zumrut Begum

PY - 2010/7

Y1 - 2010/7

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AB - Optimization of the growth conditions for maximum β-mannanase production in shake flasks by using recombinant Aspergillus sojae ATCC11906 (AsT1) was carried out by Box-Behnken design of response surface methodology. The highest β-mannanase activity on the fourth day of cultivation at 30 °C was obtained as 363 U/ml in the optimized medium consisting of 7% sugar beet molasses, 0.43% NH4NO3, 0.1% K2HPO4 and 0.05% MgSO4 (by weight per volume) at 207 rpm. On the sixth day of cultivation under the optimized conditions, the highest β-mannanase activity was achieved as 482 U/ml which is 1.4-fold of 352 U/ml activity found on glucose medium previously.

KW - Box-Behnken

KW - Endo-β-1,4-mannanase

KW - Recombinant Aspergillus sojae

KW - Response surface methodology

U2 - 10.1016/j.molcatb.2010.02.009

DO - 10.1016/j.molcatb.2010.02.009

M3 - Article

AN - SCOPUS:77952077256

SN - 1381-1177

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JO - Journal of Molecular Catalysis B: Enzymatic

JF - Journal of Molecular Catalysis B: Enzymatic

IS - 3-4

ER -

Optimal conditions for enhanced β-mannanase production by recombinant Aspergillus sojae

Abstract

Keywords

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